Development of an orofacial model of acute inflammation in the rat.

Abstract:

:An appropriate model was created by the paraperiosteal injection of mustard oil (20% allyl isothiocyanate dissolved in mineral oil) into the periarticular temporomandibular tissue of anaesthetized rats. Inflammation was assessed by the plasma extravasation of Evans' blue dye bound to plasma protein. This was confirmed visually and compared spectrophotometrically with the contralateral untreated control site (p less than 0.0005). A time-course study of the effect of mustard oil on Evans' blue extravasation revealed a gradually increasing effect that was maximal at 30 min after administration, with no further increase at 60 min. A dose-response study showed that giving 30 microliters of 20% mustard oil produced the maximal effect, with no further increase from 50 microliters. To confirm induction of inflammation, polymorphonuclear neutrophil infiltration was assessed morphometrically and found to increase in the treated tissue compared with the contralateral untreated control (p less than 0.001).

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Haas DA,Nakanishi O,MacMillan RE,Jordan RC,Hu JW

doi

10.1016/0003-9969(92)90026-5

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

417-22

issue

5

eissn

0003-9969

issn

1879-1506

pii

0003-9969(92)90026-5

journal_volume

37

pub_type

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