Purification and characterization of Porphyromonas gingivalis outer membrane antigens.

Abstract:

:Porphyromonas gingivalis is strongly associated with periodontal disease. Significant titres of specific IgG antibodies to P. gingivalis can be found in healthy individuals and those with periodontitis. In this study, 22 outer membrane antigens ranging from 15.5 to 107.6 kDa were recognized by sera from persons with periodontitis and controls. Serum from individuals with periodontitis showed a significantly higher IgG response to a 31.4-kDa antigen (p < 0.05); serum from those with gingivitis demonstrated a significantly higher response to a 15.5-kDa antigen (p < 0.05). The response to the 15.5-kDa antigen might represent a protective immune response while that to the 31.4-kDa could serve as a marker for disease susceptibility. These two antigens were purified to homogeneity and their N-terminal amino acid sequences determined. The sequences did not correspond to any previously described P. gingivalis antigens. The role of these two antigens in the pathogenesis of periodontal disease remains to be determined.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Polak B,Peck MA,Dyer JK,Bird PS,Reinhardt RA,Seymour GJ

doi

10.1016/0003-9969(95)00063-u

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

905-12

issue

10

eissn

0003-9969

issn

1879-1506

pii

000399699500063U

journal_volume

40

pub_type

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