IL-25 promotes Th2-type reactions and correlates with disease severity in the pathogenesis of oral lichen planus.


OBJECTIVE:The aim of the present study was to investigate the correlation between IL-25 expression and disease severity, and the potential immunoregulatory role of IL-25 expression in oral lichen planus (OLP). MATERIALS AND METHODS:The oral mucosal tissue samples obtained from OLP patients and healthy controls (HCs) were analyzed for IL-25 expression by real-time quantitative PCR (qPCR) and immunohistochemistry. Recombinant IL-25 was used to stimulate OLP patient-derived CD4 + T cells, and then IL-4 secretion and mRNA expression were evaluated by ELISA and qPCR, respectively. The efficiency of the siRNA-mediated knockdown of IL-25R expression in oral keratinocytes was determined by qPCR and Western blotting. Human oral keratinocyte cells were cultured with the recombinant human cytokines IL-25, IL-17 A and IL-17 F. The production of associated cytokines by keratinocytes was determined by qPCR. Statistical analyses of quantitative data were performed using SPSS software. RESULTS:The IL-25 and IL-4 mRNA levels were elevated and correlated significantly with each other in specific OLP subtype lesions compared to HCs, while the numbers of IL-25 positive cells were also increased in local OLP lesions as compared to HCs. In vitro culture with recombinant IL-25 could significantly promote CD4 + T cells from both subtypes of OLP to produce IL-4 mRNA and remarkably elevate supernatant IL-4 levels in reticular OLP CD4 + T cell cultures, which may be attributed to the elevated expression of IL-25R in local OLP lesions. Statistical analyses demonstrated that the simultaneously increased levels of IL-4, CXCL8 and CCL20 in keratinocytes were induced by IL-25 but not IL-17 A or IL-17 F. Decreasing IL-25R subunit expression by siRNA-mediated knockdown significantly blocked the expression of all cytokine-produced inflammatory mediators in oral keratinocytes. CONCLUSIONS:In OLP lesions, IL-25 can function to mediate the Th2 response in specific disease subtypes, which may be an important cause of OLP disease chronicity and persistent inflammation.


Arch Oral Biol


Archives of oral biology


Wang H,Jiang Y,Wang H,Luo Z,Wang Y,Guan X




Has Abstract


2019-02-01 00:00:00












  • Comparative immunohistochemical expression of RANK, RANKL and OPG in radicular and dentigerous cysts.

    abstract:OBJECTIVE:Receptor activator of nuclear factor-κB (RANK), RANK ligand (RANKL) and osteoprotegerin (OPG) are members of the superfamily of ligands and receptors of tumour necrosis factor family involved in bone metabolism. The formation, differentiation and activity of osteoclasts are regulated by these proteins. To cla...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: de Moraes M,de Lucena HF,de Azevedo PR,Queiroz LM,Costa Ade L

    更新日期:2011-11-01 00:00:00

  • Plasminogen activator activity in the subunits of mouse submandibular gland nerve-growth factor and epidermal growth factor.

    abstract::The 7S nerve-growth factor (7S NGF), high molecular weight epidermal growth factor (HMW-EGF) and their subunits were isolated from mouse submandibular gland. Plasminogen activator activity was found in the 7S NGF and HMW-EGF, as well as in the gamma subunit and EGF-binding protein. Later activities were two- or three-...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Hiramatsu M,Hatakeyama K,Kumegawa M,Minami N

    更新日期:1982-01-01 00:00:00

  • Porphyromonas gingivalis-induced alveolar bone loss is accelerated in the stroke-prone spontaneously hypertensive rat.

    abstract::Porphyromonas gingivalis (P. gingivalis) is one of the prominent periodontal pathogens and is the most important bacteria involved in the onset and exacerbation of periodontitis. P. gingivalis is an anaerobic, Gram-negative coccobacillus that plays a role in the progression of periodontal disease by promoting alveolar...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Tokutomi F,Wada-Takahashi S,Sugiyama S,Toyama T,Sato T,Hamada N,Tsukinoki K,Takahashi SS,Lee MC

    更新日期:2015-06-01 00:00:00

  • Fluid flow through cat dentine in vivo.

    abstract::An outward flow of fluid through exposed dentine was demonstrated in anaesthetized cats. The flow was measured by observing the movement of the fat droplets of dilute milk in a glass capillary (i.d. 30 microns) with a microscope. The capillary was sealed to the dentine with a plastic cap. The resting flow rate through...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Vongsavan N,Matthews B

    更新日期:1992-03-01 00:00:00

  • Interleukin-2 production and bone-resorption activity in vitro by unstimulated lymphocytes extracted from chronically-inflamed human periodontal tissues.

    abstract::Lymphocytes isolated from chronically-inflamed tissues were assessed for their ability to produce lymphokines without further stimulation in vitro. Cells were extracted from tissue obtained from 42 patients undergoing periodontal surgery. Cultures were set up in triplicate and supernatants collected after 48 h were as...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Seymour GJ,Cole KL,Powell RN,Lewins E,Cripps AW,Clancy RL

    更新日期:1985-01-01 00:00:00

  • Adsorption of human salivary mucins to hydroxyapatite.

    abstract::The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that M...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Tabak LA,Levine MJ,Jain NK,Bryan AR,Cohen RE,Monte LD,Zawacki S,Nancollas GH,Slomiany A,Slomiany BL

    更新日期:1985-01-01 00:00:00

  • Gingival crevicular fluid interleukin-36β (-1F8), interleukin-36γ (-1F9) and interleukin-33 (-1F11) levels in different periodontal disease.

    abstract:BACKGROUND:Periodontal inflammation is driven by the coordinated action of a number of factors, including the IL-1 family. Our study aimed to examine the levels of interleukin (IL)-36β, IL-36γ and IL-33 levels in gingival crevicular fluid (GCF) from patients with different periodontal diseases. MATERIALS AND METHODS:A...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Kurşunlu SF,Oztürk VÖ,Han B,Atmaca H,Emingil G

    更新日期:2015-01-01 00:00:00

  • Relationship of vitamin D with calbindin D9k and D28k expression in ameloblasts.

    abstract:OBJECTIVE:Calbindin D9k (CB9k) and D28k (CB28k) are intracellular soluble calcium-binding proteins, whose expressions are considered to be regulated by vitamin D. However, the amount of CB28k expression in the kidneys of vitamin D receptor-null mice was reported to be similar to that in wild type mice, suggesting no de...

    journal_title:Archives of oral biology

    pub_type: 杂志文章,评审


    authors: Onishi T,Shintani S,Wakisaka S,Ooshima T

    更新日期:2008-02-01 00:00:00

  • Relationship of masseter electromyographic activity to mandible position in the freely-moving rat.

    abstract::Simultaneous records of masseter-EMG activity and position of the mandible were made on 4 Wistar rats eating four food types: standard food pellets (large pellets), 45 mg precision food pellets (small pellets), bread and pudding. Masseter activity was recorded with chronic bipolar silver EMG electrodes placed within t...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Thomas NR,Peyton SC

    更新日期:1983-01-01 00:00:00

  • The effect of temperature on the contraction characteristics of jaw muscles in the cat.

    abstract::The effect of muscle temperature on the speed of contraction and on the strength of contraction of the cat jaw muscles was studied. Muscles contracted faster when their temperatures were increased. The tetanic and twitch tension decreased significantly as the animal was heated from 30 to 40 degrees C. ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Türker KS,Mackenna BR

    更新日期:1984-01-01 00:00:00

  • Protein synthesis and amylase messenger RNA content in rat parotid salivary glands after total or partial stimulation with isoproterenol.

    abstract::The rate of synthesis of secretory proteins increases significantly in rat parotid glands after stimulated discharge of stored proteins. How any difference in the amount of secretory protein discharge affects the rate of subsequent protein synthesis, and whether this post-secretory synthesis is regulated at the level ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Kim SK,Jones TP,Cuzzort LM

    更新日期:1989-01-01 00:00:00

  • Methionine enkephalin-like, substance P-like, and beta-endorphin-like immunoreactivity in human parotid saliva.

    abstract::These three neuropeptides were measured at daily baseline values by radioimmunoassay. Stimulated parotid saliva was collected from 31 subjects using a modified Carlson-Crittenden device affixed over Stenson's duct. Methionine enkephalin-like immunoreactivity ranged from 6.6 to 11.7 fmol/ml, with a mean of 9.3 fmol/ml....

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Pikula DL,Harris EF,Desiderio DM,Fridland GH,Lovelace JL

    更新日期:1992-09-01 00:00:00

  • Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.

    abstract:OBJECTIVE:The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN:The essential oils were characterized (GC-...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Marinković J,Ćulafić DM,Nikolić B,Đukanović S,Marković T,Tasić G,Ćirić A,Marković D

    更新日期:2020-09-01 00:00:00

  • Zinc and strontium analyses by energy dispersive X-ray fluorescence in human permanent teeth.

    abstract::The zinc and strontium content of enamel and dentine from humans aged 9-64 yr was analysed, after systematic sampling, by X-ray fluorescence. Relatively high levels of zinc were found in both hard tissues but there was no significant increase with age. Zinc content decreased significantly from outer to inner enamel an...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Frank RM,Sargentini-Maier ML,Turlot JC,Leroy MJ

    更新日期:1989-01-01 00:00:00

  • Green tea: a promising natural product in oral health.

    abstract::Green tea is a leading beverage in the Far East for thousands of years; it is regarded for a long time as a health product. Green tea is important source of polyphenol antioxidants. Polyphenols including epigallocatechin 3 gallate (EGCG) constitute the most interesting components in green tea leaves. Green tea has the...

    journal_title:Archives of oral biology

    pub_type: 杂志文章,评审


    authors: Narotzki B,Reznick AZ,Aizenbud D,Levy Y

    更新日期:2012-05-01 00:00:00

  • A comparative electron microscopic analysis of mechanoreceptors in the hard palate of the mouse (Mus musculus; Rodentia) and the musk shrew (Suncus murinus; Insectivora).

    abstract::These mechanoreceptors were studied with particular regard to their type and the distribution. In both species they were concentrated in the crests of transverse palatine rugae. In the mouse, Meissner corpuscles, glomerular corpuscles, and Merkel cell nerve endings were seen in every palatine ruga, though the first an...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Tachibana T,Fujiwara N,Sato H,Nawa T

    更新日期:1990-01-01 00:00:00

  • Expression of bone sialoprotein mRNA by cells lining the mouse tooth root during cementogenesis.

    abstract::Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: MacNeil RL,Berry J,Strayhorn C,Somerman MJ

    更新日期:1996-08-01 00:00:00

  • Immunofluorescent localization of fibronectin in human oral mucosa.

    abstract::Fibronectin (FN) with an immunohistochemical fluorescence technique using anti-human FN and anti-rat FN sera was localized in the basement membrane of lip, buccal mucosa, palate, tongue, around ectopic sebaceous glands and around acini and ducts of labial salivary glands though in different amounts. In the mucosal lam...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Linde A,Jonsson R

    更新日期:1982-01-01 00:00:00

  • Nicotine stimulates the expression of cyclooxygenase-2 mRNA via NFkappaB activation in human gingival fibroblasts.

    abstract::Nicotine, a major component in tobacco smoke, stimulates the synthesis of prostaglandin E(2). We investigated the involvement of the transcription nuclear factor kappa B (NFkappaB) in the nicotine-induced expression of cyclooxygenase-2 (COX-2), a key enzyme for prostaglandin synthesis, in human gingival fibroblasts. N...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Nakao S,Ogata Y,Sugiya H

    更新日期:2009-03-01 00:00:00

  • Studies of the chronological course of third molars eruption in a northern Chinese population.

    abstract::Dental age estimation is of great importance for individual identification in forensic medicine and many other fields of study. Among them, tooth eruption is a parameter developmental morphology that can be determined by clinical examinations or by dental X-rays. The purpose of present research is to study the chronol...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Guo YC,Yan CX,Lin XW,Zhou H,Pan F,Wei L,Tang Z,Liang F,Chen T

    更新日期:2014-09-01 00:00:00

  • Differences in the palatal rugae shape in two populations of India.

    abstract:OBJECTIVES:The aim of the study was to investigate differences in the shape of the palatal rugae in two populations of India and develop discriminant function to identify the populations based on rugae shape. METHODS:Thirty plaster casts from each population group, equally distributed between the sexes and belonging t...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Nayak P,Acharya AB,Padmini AT,Kaveri H

    更新日期:2007-10-01 00:00:00

  • Determination of NLRP3 (rs4612666) and IL-1B (rs1143634) genetic polymorphisms in periodontally diseased and healthy subjects.

    abstract:OBJECTIVE:The focus of the current study was to identify if a possible association between NLRP3 (rs4612666) and IL-1B (rs1143634) single-nucleotide polymorphisms (SNPs) may be implicated in the etiopathogenesis of chronic periodontitis (CP) in a Colombian population. DESIGN:One hundred and twenty-four CP subjects and...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Isaza-Guzmán DM,Hernández-Viana M,Bonilla-León DM,Hurtado-Cadavid MC,Tobón-Arroyave SI

    更新日期:2016-05-01 00:00:00

  • Measurement of root surface area of permanent teeth in a Chinese population.

    abstract:OBJECTIVES:The aim of this study was to investigate the relation between the remaining area of periodontal attachment and the attachment levels for each type of permanent teeth in a Chinese population by using micro-computed tomography (micro-CT) scans. DESIGN:A total of 440 extracted permanent teeth (including each t...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Gu Y,Zhu Q,Tang Y,Zhang Y,Feng X

    更新日期:2017-09-01 00:00:00

  • The relationship between microbial factors and gingival crevicular fluid glycosaminoglycans in human adult periodontitis.

    abstract::Counts of cultivable Porphyromonas gingivalis, assays of microbial proteases and the concentration in gingival crevicular fluid of proteoglycan metabolites were investigated at periodontitis and gingivitis sites in 16 patients with chronic adult periodontitis before and after treatment. Two periodontitis sites and two...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Smith AJ,Wade W,Addy M,Embery G

    更新日期:1997-01-01 00:00:00

  • Effect of gels containing chlorhexidine or epigallocatechin-3-gallate on the protein composition of the acquired enamel pellicle.

    abstract:OBJECTIVE:This study evaluated changes in protein profile of the acquired enamel pellicle (AEP) formed in vivo, after application of gels containing chlorhexidine or EGCG and further challenge with citric acid. DESIGN:AEP was formed in 9 volunteers for 2h and then treated with one of the following gels: placebo, 400μM...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: de Souza-E-Silva CM,da Silva Ventura TM,de Pau L,la Silva Cassiano,de Lima Leite A,Buzalaf MAR

    更新日期:2017-10-01 00:00:00

  • Oxidative stress indices in COPD--Broncho-alveolar lavage and salivary analysis.

    abstract:OBJECTIVE:It has been suggested that oxidative stress plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD), though this role has yet to be fully elucidated. The purpose of this study was to further evaluate this role as concomitantly expressed in the saliva and broncho-alveolar lavage (BAL/'...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Yigla M,Berkovich Y,Nagler RM

    更新日期:2007-01-01 00:00:00

  • Fluid shifts across human dentine in vitro in response to hydrodynamic stimuli.

    abstract::Most authorities agree that the hydrodynamic theory of dentine sensitivity best explains the stimulus-response relations of most painful stimuli. However, as the usual hydrodynamic stimuli are so different, it has been impossible to compare them. The equivalency of hydrodynamic stimuli can be evaluated from measuremen...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Pashley DH,Matthews WG,Zhang Y,Johnson M

    更新日期:1996-11-01 00:00:00

  • The detection of platelet-activating factor in inflamed human gingival tissue.

    abstract::Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF) is a biologically active lipid, like the prostaglandins, which mediates allergic and inflammatory reactions. Aggregation of washed rabbit platelets was induced by a lipid prepared from inflamed gingiva. The mobility of the active lipid wa...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Noguchi K,Morita I,Murota S

    更新日期:1989-01-01 00:00:00

  • Ultrastructure of granular materials in rat incisor enamel organ at early maturation stage.

    abstract::With scanning electron microscopy, globules were seen in inter-cellular spaces between ameloblasts with ruffled borders and papillary cells at early maturation stage. Numerous globules were located on longitudinal folds between parallel ridges on the sides of ameloblasts, but only a few in the papillary layer. In prev...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Yamamoto K,Matsuo S,Nishimoto T,Wakisaka S,Ichikawa H,Nakata T,Akai M

    更新日期:1984-01-01 00:00:00

  • Fluid and protein flux across the pulpodentine complex of the dog in vivo.

    abstract::Dentinal fluid volume and protein concentrations were measured from cavities prepared in the dentine of dog molars. Fluid was collected under spontaneous conditions, during the application of negative pressures, and during a recovery period. The data allowed the calculation of dentine permeability as hydraulic conduct...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Maita E,Simpson MD,Tao L,Pashley DH

    更新日期:1991-01-01 00:00:00