Expression of phosphodiesterase 3 in rat submandibular gland cell lines.

Abstract:

:A recent preliminary (unpublished) study showed that phosphodiesterase (PDE) 3A and 3B are expressed in rat submandibular glands. Here, PDE3 activity was detected in homogenates of rat submandibular gland acinar epithelial (SMIE) cells, but not rat A5 (epithelial duct) cells. Most of the PDE3 activity in SMIE cells was recovered in the particulate fraction. Only PDE3B mRNA was detected by reverse transcription-polymerase chain reaction in RNA from SMIE cells. The nucleotide sequence of the fragment was identical to the sequence of rat PDE3B. The PDE3 specific inhibitor, OPC3689 (10 and 50 microM), inhibited the growth of SMIE cells (19 and 63%), but not A5 cells. As the submandibular gland contains many types of cells, these results indicate that PDE3B may regulate a cAMP pool that is important in submandibular gland acinar epithelial cell function.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Murata T,Sugatani T,Manganiello VC,Shimizu K,Tagawa T

doi

10.1016/s0003-9969(00)00119-9

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

453-7

issue

5

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(00)00119-9

journal_volume

46

pub_type

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