Effect of an increase in occlusal vertical dimension on the rate of cyclic actin-myosin interaction in guinea-pig masseter muscle.

Abstract:

:To study the effects of increased occlusal vertical dimension on these kinetics, the actin-filament sliding velocity on masseter myosins in an in vitro motility assay and the ATPase activity of masseter myosins from normal (control) and bite-opened (5.6 mm increase in the vertical dimension for 1 week) guinea-pigs were measured. In control myosin preparations, the average value (mean +/- SD, n = 5) for the actin-filament sliding velocity at 25 degrees C was 4.0 +/- 0.3 microns/sec. In bite-opened myosin preparations (n = 5), it was 3.4 +/- 0.3 microns/sec, a significant (p < 0.01) decrease. Myosin ATPase activity was also decreased significantly (p < 0.01) from 1.0 +/- 0.1 to 0.7 +/- 0.1 mumol Pi mg per min (mean +/- SD, n = 5) after the bite opening. These results strongly suggest that in guinea-pigs an increase in occlusal vertical dimension for 1 week decreases the turnover rate of actin-myosin interaction in the masseter through changes in the myosin isozyme. These changes may result in a slowing of the rate of detachment of myosin cross-bridges from actin filaments.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Kawasaki K,Saeki Y,Ohnuki Y

doi

10.1016/s0003-9969(97)00038-1

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

505-12

issue

7

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(97)00038-1

journal_volume

42

pub_type

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