Abstract:
OBJECTIVE:Macrophages are involved in tissue inflammation and repair through cytokine secretion. However, the contribution of macrophages to healing and osteogenesis after tooth extraction remains unclear. Therefore, we investigated the distribution of osteoblastic cells and macrophages in the early healing process after tooth extraction. METHODS:The maxillary first molars of 6-week-old male mice were extracted. The maxilla was collected 1, 3, and 7 days after extraction. The states of socket healing, localization of osteoblastic markers, and macrophage infiltration were sequentially observed by micro-CT imaging and immunohistochemistry. RESULTS:On day 3 after tooth extraction, α-smooth muscle actin (SMA)-positive cells, osteoprogenitor cells at fracture healing, were observed in the socket. Several α-SMA-positive cells also expressed Runx2, the early osteoblast differentiation marker. The infiltration of F4/80-positive, mature macrophages and CD206-positive, M2-like macrophages was noted in the socket. However, CD169-positive macrophages (Osteomac), which are involved in fracture healing, were not detected in the socket. F4/80-positive and CD206-positive macrophages also showed the localization of transforming growth factor-β (TGF-β), which promotes osteoprogenitor cell proliferation and early differentiation. Phosphorylated Smad3, a downstream mediator of the signal activity of TGF-β, was detected in α-SMA-positive cells. On day 7, the extracted socket contained a large amount of new bone. Tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts were detected on bone surfaces. CONCLUSION:Our data indicate that M2-like macrophages regulate the proliferation and differentiation of α-SMA-positive cells by secreting TGF-β at the early stage of socket healing, and also suggest the importance of macrophages in healing and bone formation after tooth extraction.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Horibe K,Hara M,Nakamura Hdoi
10.1016/j.archoralbio.2021.105042subject
Has Abstractpub_date
2021-01-08 00:00:00pages
105042eissn
0003-9969issn
1879-1506pii
S0003-9969(21)00005-4journal_volume
123pub_type
杂志文章abstract::Physiological evidence is presented for the existence of commissural fibres that cross the midsagittal plane in the medulla of the rat at the level of the trigeminal motor nucleus (Mo5). These fibres, which have their origin in the Mo5, terminated in the contralateral Mo5. Small inhibitory postsynaptic potentials were...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90170-q
更新日期:1993-12-01 00:00:00
abstract::An outward flow of fluid through exposed dentine was demonstrated in anaesthetized cats. The flow was measured by observing the movement of the fat droplets of dilute milk in a glass capillary (i.d. 30 microns) with a microscope. The capillary was sealed to the dentine with a plastic cap. The resting flow rate through...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90087-o
更新日期:1992-03-01 00:00:00
abstract:OBJECTIVE:Intraperitoneal injection of a sialogogue, pilocarpine, at high concentrations induces salivation via peripheral pathways and thirst sensation via central pathways. In this study, we report that the effects of another sialagogue, cevimeline, on salivation and water intake in conscious rats differ from those o...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2006.09.002
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:Previous evaluations of Psidium cattleianum leaf extract were not done in conditions similar to the oral environment. The aim of this study was to evaluate the effect of P. cattleianum leaf extract on enamel demineralisation, extracellular polysaccharide formation, and the microbial composition of dental biof...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.02.009
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVE:Amelogenesis imperfecta (AI) is a group of clinically and genetically heterogeneous inherited conditions, causing alterations in the structure of enamel and chemical composition of enamel matrix during development. The objective of this study was to compare the clinical, radiographic, histological and immunoh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.06.016
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:This study was conducted to identify and characterize dental follicle stem cells (DFSCs) by analyzing expression of embryonic, mesenchymal and neural stem cells surface markers. Design Dental follicle cells (DFCs) were evaluated by immunocytochemistry using embryonic stem cells markers (OCT4 and SOX2), mesenc...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.10.003
更新日期:2017-01-01 00:00:00
abstract::The surface zone of human deciduous and permanent enamel was examined following sectioning, polishing and etching. Three main types of surface were where prisms reached the surface, where the prisms were not discernible and those formed by a complex of both types. The aprismatic layers were measured and the percentage...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90147-9
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to clarify the effects of zinc chloride (ZnCl2) exposure on the induction of heme oxygenase-1 (HO-1) expression and its regulatory mechanisms in MDPC-23 mouse odontoblast-like cells. METHODS:MDPC-23 cells were incubated with ZnCl2, and the levels of HO-1 protein, phosphorylated forms...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.09.002
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVES:The present study aimed firstly, to investigate whether pulpal circulatory changes elicited by innocuous tooth cooling and foot heating can be monitored with transmitted-light plethysmography (TLP), which detects pulpal blood volume changes, and secondly, to assess the effect of autonomic nervous control on ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104881
更新日期:2020-11-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.06.010
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Interspecies interactions of oral streptococci involve the production and excretion of antimicrobial compounds to compete successfully during colonization. Bacteriocin production by Streptococcus mutans and hydrogen peroxide (H2O2) production by Streptococcus sanguinis have been demonstrated as crucial for th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.03.012
更新日期:2010-05-01 00:00:00
abstract::The classical hydrodynamic theory implicated fluid movement as a transducing mechanism in the production of dental sensitivity. This theory assumes that sensitive dentine must be permeable. Various measurements of dentine permeability are discussed, including: (1) factors that influence diffusive permeation across den...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/0003-9969(94)90191-0
更新日期:1994-01-01 00:00:00
abstract::Gland size, amylase activity, total proteins and DNA concentrations were measured in parotid gland during chronic heat exposure (34 degrees C). Subcellular fractionation was performed. Exposure to heat resulted in a decrease in gland size and in DNA concentration; amylase activity/100 mg tissue and amylase specific ac...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90059-6
更新日期:1983-01-01 00:00:00
abstract::Normal function of the lateral pterygoid muscle is not well understood. The principal aim of this study was to determine whether there is a progressive decrease in lateral pterygoid activity as the condyle moves posteriorly and superiorly during the return phase of a contralateral or protrusive jaw movement, as would ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00011-x
更新日期:2000-06-01 00:00:00
abstract::This report concerns changes brought about in the submandibular salivary gland during different stages of the oestrous cycle in the concentrations of total lipids, cholesterol and protein, together with an assessment of the activities of cyclic AMP-specific phosphodiesterase, ATPase, succinate dehydrogenase and an est...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(96)00011-8
更新日期:1996-02-01 00:00:00
abstract::Fifty 4- to 6-week-old male random-bred golden hamsters were injected intraperitoneally with a weight-related dose (12.5 mg/kg body weight) of N-methyl-N-nitrosourea (NMU) three times a week for 4 weeks. Groups of seven animals were killed 10, 16 and 22 weeks after the first injection. The palatal gingiva from six ani...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90149-x
更新日期:1994-06-01 00:00:00
abstract:OBJECTIVE:To clarify the fluoride/mineral kinetics in an oral biofilm following concurrent application of fluoride and other mineral ions released from experimental toothpaste containing S-PRG filler using depth-specific analysis. STUDY DESIGN:Twenty subjects wore in situ plaque-generating devices, comprised of a pair...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104777
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:When eating solids, stage II transport (St2Tr) propels triturated food into the pharynx for bolus formation and storage before swallowing. Although the existence of St2Tr is acknowledged, the reason for its existence remains unclear. Understanding it may facilitate development of food appropriate for individu...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.05.013
更新日期:2017-09-01 00:00:00
abstract::A phenotypic study had shown that gingival B cells respond differently to two periodontopathic bacteria, Porphyromonas gingivalis and Fusobacterium nucleatum. Further investigation now shows a reduction in the percentage of Ki-67 + T cells in cultures of gingival and peripheral blood mononuclear cells stimulated with ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90139-y
更新日期:1992-01-01 00:00:00
abstract::Human salivary alpha-amylase participates in the initial digestion of starch and may be involved in the colonization of viridans streptococci in the mouth. To elucidate the role of histidine residues located near the starch-binding site on the streptococcal-binding activity, the wild type and three histidine mutants, ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00112-5
更新日期:1999-02-01 00:00:00
abstract::The change in expression of MUC1 from health to disease forms the basis of its use as a potential disease marker. Previous attempts at isolating MUC1 from normal, healthy human oral mucosa have, however, drawn conflicting conclusions as to its presence. Furthermore, when MUC1 was detected in the oral glycocalyx, it wa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00010-3
更新日期:2001-06-01 00:00:00
abstract::The purpose was to examine the effect of brief intrusive forces on human pulpal blood flow (PBF). Laser Doppler flowmetric measurements were made from 17 vital upper left central incisors of 17 participants who had clinically healthy tooth crowns and periodontal tissues. Brief intrusive forces (0.5,1,5 N; duration 20 ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00049-8
更新日期:2001-09-01 00:00:00
abstract:OBJECTIVE:Tooth eruption is a multifactorial process in which bone tissue plays a prevailing role. In this study we evaluated the bone overlying the developing tooth germ and the degree of tooth eruption of the first mandibular molar in pups born to mothers subjected to constant light during pregnancy. DESIGN:Pregnant...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.08.003
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To test the hypothesis that experimental occlusal interferences increase the nerve growth factor (NGF) levels in periodontal tissues and cause an up-regulation of preprotachykinin-A (PPTA) mRNA. BACKGROUND:NGF is related to hyperalgesia and inflammation. PPTA mRNA, a primer of substance P, is a possible fact...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.08.007
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Dental pulp repair is a common process triggered by microbial and mechanical challenges. Matricellular modulators, such as periostin, are key for extracellular matrix stability and tissue healing. In the scope of the dental pulp, periostin expression has been reported during development and act...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.02.008
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVE:This in vitro study was undertaken to assess the effects of Galla chinensis extract on inhibition of enamel caries-like demineralization and to elucidate the role of the organic matrix of enamel in this process. DESIGN:Either regular or enamel disposed of its organic matrix both of bovine origin were exposed...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2009.06.007
更新日期:2009-09-01 00:00:00
abstract::Normal keratinocytes from epidermis and from buccal mucosa underwent dissimilar stages of differentiation in the same culture medium and responded differently to changes in the composition of the medium. Manifestations of these variations were examined in terms of the expression at the mRNA level (as measured by rever...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00046-r
更新日期:1995-09-01 00:00:00
abstract:OBJECTIVES:This study evaluated the level and mechanism of apoptosis in human gingival fibroblasts (HGF) and murine fibroblasts (NIH/3T3) treated with a titanium tetrafluoride (TiF4) varnish compared those treated with a sodium fluoride (NaF) varnish. METHODS:Cells were treated with a TiF4, NaF (both 2.45%F) or placeb...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.10.039
更新日期:2019-01-01 00:00:00
abstract::Growth in body length, skeletal lengths, incisor lengths and molar root lengths were retarded in dwarf mice. The root lengths of third molars were more severely affected than those of other molars. The crown lengths at the enamel-cementum junction of upper and lower molars of most dwarf mice were significantly shorter...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90135-9
更新日期:1985-01-01 00:00:00
abstract::Destrin, an actin-binding protein, is partly phosphorylated at Ser-2 (numbering on the matured form) in the resting rat parotid gland, and beta-adrenergic or cholinergic stimulation of this gland induces its dephosphorylation. In this study, we searched for the protein kinase involved in phosphorylation of destrin. We...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(03)00129-8
更新日期:2003-09-01 00:00:00