Low molecular-weight proteins in human gingival crevicular fluid.

Abstract:

:Samples of gingival crevicular fluid (GCF) were collected in 30 volunteers with inflamed gingiva, using either capillary tubes (cGCF) or Durapore strips (sGCF). They were examined, together with samples of serum from the same patients, by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and/or by two-dimensional electrophoresis, followed by silver staining. The results confirmed that the distribution of the major proteins in GCF is similar to that found in serum. However, an 8.5 kDa protein was found in gingival fluid but not in serum. The low molecular-weight protein appears to decrease with time of fluid sampling with both techniques, and does not originate either from blood or from the cellular fraction of GCF. Two-dimensional electrophoretic analysis suggested that it may consist of several polypeptides.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Estreicher A,Broggiato A,Duroux P,Andersen E,Cimasoni G

doi

10.1016/s0003-9969(96)00076-3

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

733-8

issue

8-9

eissn

0003-9969

issn

1879-1506

journal_volume

41

pub_type

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