Abstract:
OBJECTIVE:Preservation of structural and biochemical properties of the root dentin matrix is crucial to favor healing and regenerative periodontal processes. Aim of this study was to evaluate the biochemical characteristics of collagen and chondroitin sulphate of root dentin surfaces exposed by periodontal disease after acid conditioning by means of an immunohistochemical technique. DESIGN:Human teeth scheduled for extraction due to periodontal reason were submitted to: (A) scaling and root planning; (B) ultrasonic instrumentation; (C) no instrumentation. Teeth were then exposed to: (1) 10% citric acid; (2) 17% EDTA; (3) no etching. A double immunolabeling technique was performed to identify type-I collagen and proteoglycans and analyzed under FEI-SEM. RESULTS:Use of 10% citric acid revealed intense labeling for collagen fibrils and proteoglycans; lower labeling was found after EDTA conditioning. Unetched specimens showed residual smear layer on the dentin surface resulting in no evident surface labeling. CONCLUSIONS:This study supports the hypothesis that manual or ultrasonic instrumentation alone is not able to expose the sound dentin matrix, whereas a subsequent acidic conditioning exposes collagen fibrils and associated proteoglycans. The immunohistochemical technique revealed that despite their acidity, both citric acid and EDTA were able to preserve the structural and biochemical properties of the exposed dentin matrix.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Ruggeri A Jr,Prati C,Mazzoni A,Nucci C,Di Lenarda R,Mazzotti G,Breschi Ldoi
10.1016/j.archoralbio.2006.07.001subject
Has Abstractpub_date
2007-01-01 00:00:00pages
1-8issue
1eissn
0003-9969issn
1879-1506pii
S0003-9969(06)00205-6journal_volume
52pub_type
杂志文章abstract::Herpes simplex virus type 1 (HSV1) can be rapidly identified in saliva from patients with acute herpetic gingivostomatitis, by in vitro amplification using the polymerase chain reaction and specific primers. Amplification of DNA results in a product of 110 bp length corresponding to the region 1381-1490 bp of the HSV1...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90113-m
更新日期:1992-10-01 00:00:00
abstract:OBJECTIVE:A loss of functional salivary glands often occurs after radiotherapy for head and neck tumour, and causes many problems in oral health. Recently, the use of salispheres, which consist of salivary gland stem cells (SGSCs), has been suggested as therapy for these problems. However, an insufficient number of cel...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.03.003
更新日期:2015-06-01 00:00:00
abstract::The proteolytic activity of N-benzoyl-D,L-arginine-p-nitroanilide hydrochloride, a trypsin-like protease, was weak, whereas strong glucose-6-phosphate dehydrogenase activity was found in all subjects. The enzyme activities of males and females were similar. ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90124-5
更新日期:1987-01-01 00:00:00
abstract::Periodontal bone loss was induced in mice of different genotypes by oral inoculation with Actinomyces viscosus. Relatively high bone loss occurred in the mutant X-linked immune deficiency, implicating the humoral immune system. Maximum bone loss was always associated with inoculations of 1.6 x 10(7) colony forming uni...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90013-1
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:To compare the tissue reactions to implanted collagen scaffolds in the palate and the skin of rats. DESIGN:Crosslinked collagen scaffolds were implanted submucoperiosteally in the palate, and subcutaneously on the skull and on the back of 25 rats and evaluated after up to 16 weeks. On H&E-stained sections, t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.11.003
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVE:Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.05.011
更新日期:2017-09-01 00:00:00
abstract::The fragmentation of foods (breakage function) was measured in five humans on "bagged' single particles of 28 foods from three food groups. The change in the square root of the specific surface of the particles (the specific surface being the area of particle silhouettes, measured by image analysis, divided by origina...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00102-1
更新日期:1997-01-01 00:00:00
abstract::Apatite crystal growth rate and morphology in mineralized tissues are considered to be controlled by surface interaction with extracellular matrix proteins. During enamel maturation where protein is finally removed from crystal surfaces to permit massive crystal growth, pH oscillates between approximately 5.8 and appr...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.11.017
更新日期:2005-02-01 00:00:00
abstract::Subdividing the cheek, palate and ventral tongue into seven anatomically identifiable regions enabled us to assess relationships that were previously obscured by the great heterogeneity of rat oral epithelium. Capillaries were visualized by an alkaline phosphatase azo dye method in oral mucosa of four 3-month old male...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90058-9
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:To clarify whether over-eruption of the mandibular third molar can disturb chewing movement. METHODS:Eighteen patients with a unilateral mandibular over-erupted third molar confirmed by both study cast observation and T-scan occlusal detection were selected from a sample of patients with complaints of tempor...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.06.012
更新日期:2017-10-01 00:00:00
abstract::Various fruit juices with relatively low pH are known to have erosive effects on human tooth enamel in a reasonably short time. Honey, also with a relatively low pH, could do the same, but scanning electron microscopy showed no erosion of enamel by honey over a period of 30 min, neither did Knoop microhardness tests s...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90110-4
更新日期:1994-02-01 00:00:00
abstract::Natural immunity to synthetic peptides (SP) derived from the sequences of a 3800 Mr Streptococcus mutans antigen was found in human subjects. Significant serum IgG antibodies were detected both to the native streptococcal antigen and to the SP17, containing essentially residues 1-15. A series of short peptides with de...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90129-x
更新日期:1990-01-01 00:00:00
abstract::Age-related changes in the content of non-reducible crosslink amino acids, pyridinoline and histidinoalanine, in rat mandibular bone were studied. The pyridinoline content markedly increased up to 12 months of age and thereafter slightly increased. The histidinoalanine content was low until 3 months of age, but therea...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90075-5
更新日期:1985-01-01 00:00:00
abstract:OBJECTIVE:Dental follicle cells (DFCs) are the genuine precursors of alveolar osteoblasts. Previous studies suggested that collagen I supports the osteogenic differentiation of DFCs. This study investigated the effect of collagen I on the osteogenic differentiation of human DFCs. MATERIALS AND METHODS:We modified the ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.07.013
更新日期:2014-12-01 00:00:00
abstract::The 7S nerve-growth factor (7S NGF), high molecular weight epidermal growth factor (HMW-EGF) and their subunits were isolated from mouse submandibular gland. Plasminogen activator activity was found in the 7S NGF and HMW-EGF, as well as in the gamma subunit and EGF-binding protein. Later activities were two- or three-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90094-2
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:To investigate the functional mechanism of microRNA-182 (miR-182) in head and neck squamous cell carcinoma (HNSCC). DESIGN:HNSCC and normal samples were used to check both p53 and miR-182 expression. Two cancer cell lines with overexpression of miR-182 were used to check cell proliferation and migration. In ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.09.012
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVES:Reconstruct childhood diet using teeth collected in Edmonton in a collaborative effort between the Departments of Dentistry and Anthropology at the University of Alberta. METHODS:Deciduous teeth needing extraction were collected from 33 children for stable isotope ratio analysis of diet. Tooth dentin was mi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.07.014
更新日期:2014-11-01 00:00:00
abstract::In the majority of studies on masticatory performance, the degree of food breakdown has been determined by sieving the food with one or more sieves. The aim of the present study was to compare the results obtained with a single and a multiple sieve method, as these methods have never been compared. We analysed the che...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2003.08.007
更新日期:2004-03-01 00:00:00
abstract::Internal derangement (ID) in the temporomandibular joint (TMJ) comprises a group of clinical problems with relatively high prevalence. However, the temporal changes in gene expression of condylar cartilage during continuous ID remain unclear. The aim of the current study is to investigate by microarray analysis, the d...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.11.006
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:Recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. The purpose of the p...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2009.01.010
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. DESIGN:We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal days 1-100 by ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.09.007
更新日期:2006-05-01 00:00:00
abstract::The accessory innervation of the mandibular dentition was examined by use of the retrograde transportation of horseradish peroxidase or wheat-germ agglutinin-horseradish peroxidase. Nerves within the cervical plexus which contained transported horseradish peroxidase included the great auricular and transverse cervical...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90129-a
更新日期:1993-07-01 00:00:00
abstract::The purpose of this study is to investigate the radioprotective effect of vitamin E as a natural product. Vitamin E protects the salivary glands dysfunction that is induced by ionizing radiation. It was analysed with radioisotope scintigraphy and then salivary gland to background counts ratio was calculated. Histopath...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.06.003
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVES:The influence of carotid artery occlusion (10, 30 and 60 min) on regulatory mechanisms implicated in the vasorelaxant responses of isolated glandular branch of rabbit facial artery to acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) was examined. DESIGN:In organ bath studies with arterial rin...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.03.006
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE:The biofilm of Streptococcus mutans is associated with induction of dental caries. Also, they produce glucan as an extracellular polysaccharide through glucosyltransferases and help the formation of cariogenic biofilm. β-caryophyllene has been used for therapeutic agent in traditional medicine and has antimic...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.01.009
更新日期:2018-04-01 00:00:00
abstract::Autoantibodies directed against nucleic acid and protein complexes present in cell nuclei characterize autoimmune diseases and are employed in diagnosis. The mechanisms by which these autoantigens escape immunological tolerance are largely unknown, but a number of recent observations suggest that modified self-protein...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00025-0
更新日期:2002-06-01 00:00:00
abstract::To test the hypothesis that human tooth eruption requires a critical time period during which no force is applied to the erupting tooth, the eruption of a maxillary second premolar in the prefunctional stage was recorded during the afternoon or evening hours in eight children, using an optical device based on the prin...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00011-4
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVE:Oral lichen planus (OLP) presents with large numbers of T lymphocytes accumulating beneath the epithelium of the oral mucosa; however, its aetiology remains obscure. A potential role for an emerging novel T cell subset, Th9, in OLP has recently been suggested but remains to be clarified. The current aim was t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.003
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:Interleukin-1 (IL-1) is a proinflammatory cytokine that is highly elevated in response to bacterial biofilms and is a potential risk factor for periodontal diseases. IL-1 gene polymorphisms have been associated with the IL-1 level. The aim of this study was to investigate if IL-1 gene cluster polymorphisms ar...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.10.001
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:Interspecies interactions of oral streptococci involve the production and excretion of antimicrobial compounds to compete successfully during colonization. Bacteriocin production by Streptococcus mutans and hydrogen peroxide (H2O2) production by Streptococcus sanguinis have been demonstrated as crucial for th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.03.012
更新日期:2010-05-01 00:00:00