Abstract：
:Natural immunity to synthetic peptides (SP) derived from the sequences of a 3800 Mr Streptococcus mutans antigen was found in human subjects. Significant serum IgG antibodies were detected both to the native streptococcal antigen and to the SP17, containing essentially residues 1-15. A series of short peptides with deletions at the amino- and carboxy-termini were then tested to identify the B-cell epitopes. Residues 8-13 and 1-6 bound significant serum IgG antibodies but only the former consistently inhibited human antibodies, suggesting that residues 8-13 constitute a major B-cell epitope. The human CD4 subset of T-cells was then examined and this showed a significant uptake of [3H]-thymidine when stimulated with both the native streptococcal antigen and the SP17. The series of short peptides was then used to stimulate CD4 cells, in order to determine the T-cell epitope. The synthetic peptide with residues 6-15 was the shortest peptide that stimulated significant [3H]-thymidine uptake and this peptide was designated as a T-cell epitope. The immunogenicity and antigenicity of SP17 was also investigated in macaques. Immunization of monkeys with the free SP17 failed to elicit serum antibodies or T-cell responses. However, immunization with SP17 linked to tetanus toxoid as a carrier elicited serum antibodies and proliferative responses of lymphocytes, not only to the synthetic peptide but also to the native streptococcal antigen. As in the human studies a B-cell epitope was found in residues 8-13, whereas an overlapping T-cell epitope was located in residues 7-15.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Lehner T,Walker P,Smerdon R,Childerstone A,Bergmeier LA,Haron Jdoi
10.1016/0003-9969(90)90129-xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
39S-45Seissn
0003-9969issn
1879-1506journal_volume
35 Supplpub_type
杂志文章abstract：:Specific, well-characterized antisera to the extracellular matrix glycoprotein tenascin were used. Immunoreactivity was detected in association with dentinal tubules; it was particularly prominent in the tooth crown, and was stronger within matrix than within predentine. In contrast, there was no anti-fibronectin stai...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90080-e
更新日期：1991-01-01 00:00:00
abstract：:Two mechanisms that contribute to tooth eruption are proposed from analysis of the human jaws as elastic structures which are deformed intermittently under functional occlusal loads. One possible source of eruptive force is the transient pressure gradient established within each jaw as it is deformed. The second sourc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90093-8
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:Previous evaluations of Psidium cattleianum leaf extract were not done in conditions similar to the oral environment. The aim of this study was to evaluate the effect of P. cattleianum leaf extract on enamel demineralisation, extracellular polysaccharide formation, and the microbial composition of dental biof...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.02.009
更新日期：2012-08-01 00:00:00
abstract：BACKGROUND:Interferon gamma (IFN-γ) is one of the key regulatory cytokines that has a significant effect on immune responses. It may be important in the chronic inflammatory diseases such as periodontitis in which increased IFN-γ levels were found. The aim of this study was to analyze +874A/T polymorphism in the IFN-γ ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.09.005
更新日期：2011-02-01 00:00:00
abstract：:The purpose was to examine the effect of brief intrusive forces on human pulpal blood flow (PBF). Laser Doppler flowmetric measurements were made from 17 vital upper left central incisors of 17 participants who had clinically healthy tooth crowns and periodontal tissues. Brief intrusive forces (0.5,1,5 N; duration 20 ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00049-8
更新日期：2001-09-01 00:00:00
abstract：OBJECTIVES:The aim of the present study was to identify the anticancer effects and the mechanisms of action of shikonin and its analogue isobutyrylshikonin in oral squamous carcinoma cells. DESIGNS:The cytotoxic effects of isobutyrylshikonin and shikonin in Ca9-22 and SCC-25 cells were analyzed using 3-(4,5-dimethylth...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104774
更新日期：2020-08-01 00:00:00
abstract：:In the majority of studies on masticatory performance, the degree of food breakdown has been determined by sieving the food with one or more sieves. The aim of the present study was to compare the results obtained with a single and a multiple sieve method, as these methods have never been compared. We analysed the che...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2003.08.007
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:Validation of a flow cytometry-based method for the determination of major leucocyte subsets [polymorphonuclear (PMN) cells, monocytes, T cells and B cells] in paraffin-stimulated whole human saliva. DESIGN:Salivary leucocyte subsets were determined by four-colour flow cytometry in eight healthy volunteers o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.10.015
更新日期：2012-05-01 00:00:00
abstract：:Macrophage inflammatory protein-3alpha (MIP-3alpha or CCL20) is an intriguing molecule in cancer immunotherapy, but MIP-3alpha expression and signalling are not well understood in oral cancer cells. We investigated CCL20 expression and signal transduction by treating immortalized human oral keratinocyte (IHOK) and ora...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.01.015
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to clarify the interaction of vascular endothelial growth factors (VEGFs)-C and -D with cell surface foetal liver kinase-1 (Flk-1) and fms-like tyrosine kinase-4 (Flt-4) receptors in the induction and activity of osteoclasts in cultured human peripheral blood mononuclear cells (P...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.06.010
更新日期：2013-01-01 00:00:00
abstract：:One dentine specimen was prepared from each of 90 bovine incisors. The samples were then evenly distributed among nine groups (A-I) and submitted to 10 alternating de- and re-mineralization cycles, including abrasion by tooth brushing. Each cycle started with a demineralization using the erosive soft drink Sprite Ligh...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00039-5
更新日期：2001-09-01 00:00:00
abstract：:Thirty-nine different microorganisms commonly found in supragingival plaque and salivary sediment were screened for their ability to raise the pH by producing base from arginine, lysylarginine and urea. Only Actinomyces naeslundii and Staphylococcus epidermidis showed significant pH-rise activity with all three compou...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90045-9
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVES:brain-derived neurotrophic factor (BDNF), which is produced in rat submandibular gland, is one of the most abundant neurotrophins in the central nervous system. It is generally accepted that occlusal disharmony causes stress. The purpose of the present study was to investigate whether occlusal disharmony-ind...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.09.001
更新日期：2011-01-01 00:00:00
abstract：:The aim was to examine the nature of enamel dissolution in aqueous suspensions with a high solid/solution ratio and in a CO2-rich atmosphere. Before experimentation, a water-saturated mixture of 95% N2-5% CO2 was passed through the acid solutions for 24 hr. Samples of 2 g of powdered enamel were suspended in 7 ml of e...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00043-5
更新日期：1997-09-01 00:00:00
abstract：OBJECTIVE:To study the potential chemotherapeutic effects of Diosgenin, zoledronic acid and Epigallocatechin-3-gallate on oral squamous cell cancer (OSCC). MATERIALS AND METHODS:Cell viability, migration, apoptosis and cell cycle evaluation assays were performed in order to assess the effects of different doses of Dio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.05.023
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:Interspecies interactions of oral streptococci involve the production and excretion of antimicrobial compounds to compete successfully during colonization. Bacteriocin production by Streptococcus mutans and hydrogen peroxide (H2O2) production by Streptococcus sanguinis have been demonstrated as crucial for th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.03.012
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:This study reports the expression of proinflammatory cytokines in temporomandibular joint (TMJ) of patients affected with temporomandibular osteoarthritis (OA). DESIGN:In twelve OA of the TMJ (OA-TMJ) affected patients and in six healthy volunteer subjects studied as control, the expression of IL1beta (inter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.04.004
更新日期：2008-10-01 00:00:00
abstract：:The lesion of microhypoplasia of an unerupted tooth was composed of small, irregularly distributed crystals, similar to those of immature and hypomineralized enamel. The lesion appeared to be a defect of crystal growth and maturation, probably involving a partial and momentary failure of the operating cycle of an amel...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90055-4
更新日期：1984-01-01 00:00:00
abstract：:Perfusion with a variety of commonly-used fixatives was used. Quality of cell preservation and occurrence of stippled material between the mineralization front and the secretory ameloblasts were compared in normal animals and in ones injected with fluoride. There was no relationship between the formation of stippled m...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90172-9
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:It was aimed to investigate the in vivo effects of local injection of sclerostin protein on orthodontic tooth movement. DESIGN:A total of 48 rats underwent orthodontic mesialization of the maxillary first molars on both sides. Local injection was given at the compression side in the alveolar bone on both max...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.12.011
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:The study aimed at evaluating in vitro the effect of caffeine on expression of cystatin SN, a potential marker of sensitivity to bitterness in humans. METHODS:Differentiation of human submandibular gland (HSG) cells was induced by culturing cells on Matrigel. Caffeine cytotoxicity was assessed over 3 days by...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.06.005
更新日期：2013-10-01 00:00:00
abstract：:The aetiology of oral premalignant lesions is unknown. Oxidative stress is associated with inflammation and cancerogenesis. The aim of our study was to compare salivary markers of oxidative and carbonyl stress in patients with oral premalignant lesions and age-matched healthy controls. Unstimulated saliva samples were...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.09.003
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:Recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. The purpose of the p...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.01.010
更新日期：2009-04-01 00:00:00
abstract：:Salivary gland intralobular ducts are responsible for the modification of the electrolyte composition of the primary fluid secreted by the acini. However, the intracellular messengers that regulate this and other intralobular duct cell processes have not been fully characterized. To investigate the possibility that cA...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00028-3
更新日期：1996-07-01 00:00:00
abstract：OBJECTIVE:This study evaluated changes in protein profile of the acquired enamel pellicle (AEP) formed in vivo, after application of gels containing chlorhexidine or EGCG and further challenge with citric acid. DESIGN:AEP was formed in 9 volunteers for 2h and then treated with one of the following gels: placebo, 400μM...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.05.024
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. METHODS:S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. U...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.01.004
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:While Aggregatibacter actinomycetemcomitans (Aa) is highly associated with localised aggressive periodontitis (LAP) many Aa-carriers do not develop LAP. This study was designed to determine whether specific salivary factors could distinguish between subjects who have Aa initially and remain healthy (H/AA) as ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.10.009
更新日期：2013-06-01 00:00:00
abstract：:The 10,000 g supernatant of wax-stimulated whole saliva was fractionated by gel filtration and its components were tested along with amino acids, small peptides and urea for their ability to stimulate this oxygen uptake, and for their effects on pH. Fractions containing the larger components, the proteins and large pe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90176-b
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Dental pulp repair is a common process triggered by microbial and mechanical challenges. Matricellular modulators, such as periostin, are key for extracellular matrix stability and tissue healing. In the scope of the dental pulp, periostin expression has been reported during development and act...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.02.008
更新日期：2015-05-01 00:00:00
abstract：:The functioning lower jaw is a prerequisite of the ongoing secondary chondrogenesis in the mammalian mandibular condyle. Does the articular function also initiate secondary chondrogenesis in the mandible? The angular process of the fetal mammalian mandible possesses a large secondary cartilage without any apparent art...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90154-e
更新日期：1993-01-01 00:00:00
