Abstract：
OBJECTIVE:The purpose of this study was to clarify the interaction of vascular endothelial growth factors (VEGFs)-C and -D with cell surface foetal liver kinase-1 (Flk-1) and fms-like tyrosine kinase-4 (Flt-4) receptors in the induction and activity of osteoclasts in cultured human peripheral blood mononuclear cells (PBMCs). DESIGN:PBMCs were cultured on chamber slides or on ivory discs for 2 or 3 weeks in the presence of macrophage-colony stimulating factor (M-CSF), VEGF-A, -C or -D, or placental growth factor (PlGF) with or without receptor activator of nuclear factor kappa-B ligand (RANKL). The number of osteoclasts in each group was counted and the area of ivory resorption was measured. In addition, osteoclast differentiation was further analysed under the same conditions, but with the addition of specific neutralizing antibodies against Flk-1 and Flt-4. RESULTS:RANKL was essential for the induction of osteoclasts in PBMCs. However, significant differences were found in the number of osteoclasts induced by VEGF-A, -C, -D or M-CSF with RANKL compared with control groups lacking or containing RANKL. Blocking of either Flk-1 or Flt-4 resulted in a reduction in the enhancement of osteoclast differentiation in PBMCs by VEGF-C or -D with RANKL. The osteoclasts induced by VEGF-A, -C, -D or M-CSF with RANKL formed significantly larger resorption lacunae than those formed by osteoclasts induced by RANKL alone. CONCLUSIONS:This study showed that VEGF-C and -D play a role in the induction of osteoclast differentiation through both Flk-1 and Flt-4 receptors and influence the area of the ivory resorption in PBMCs.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Motokawa M,Tsuka N,Kaku M,Kawata T,Fujita T,Ohtani J,Matsuda Y,Terao A,Tanne Kdoi
10.1016/j.archoralbio.2012.06.010subject
Has Abstractpub_date
2013-01-01 00:00:00pages
35-41issue
1eissn
0003-9969issn
1879-1506pii
S0003-9969(12)00217-8journal_volume
58pub_type
杂志文章abstract：UNLABELLED:Growth hormone (GH) stimulates mandibular growth but its effect on the mandibular condylar cartilage is not well understood. OBJECTIVE:This study was designed to understand the influence of GH on mitotic activity and on chondrocytes maturation. The effect of GH on cartilage thickness was also determined. D...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.02.004
更新日期：2004-07-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of sitagliptin, a dipeptidyl peptidase 4 inhibitor used to treat type II diabetes, on bone tissue and on implant osseointegration in diabetic rats. DESIGN:Thirty-two male rats were divided into four groups: 1) Diabetic animals (GD); 2) Diabetic animals that received sitagliptin (GD...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.04.018
更新日期：2019-06-01 00:00:00
abstract：:A genomic library of Bacteroides gingivalis W83 chromosomal DNA was constructed in the Escherichia coli lambda (lambda) vector EMBL 4. Three recombinant lambda phages expressing a cloned protease were identified in the library. All three lambda phages contained cloned overlapping DNA fragments from the same region of ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90137-y
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:to evaluate the association between XPD and XRCC3 polymorphisms and oral squamous cell carcinoma (OSCC). DESIGN:the sample consisted of 54 cases of OSCC and 40 cases of inflammatory fibrous hyperplasia (IFH). Genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.12.004
更新日期：2016-04-01 00:00:00
abstract：:Apatite crystal growth rate and morphology in mineralized tissues are considered to be controlled by surface interaction with extracellular matrix proteins. During enamel maturation where protein is finally removed from crystal surfaces to permit massive crystal growth, pH oscillates between approximately 5.8 and appr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.11.017
更新日期：2005-02-01 00:00:00
abstract：:Gland size, amylase activity, total proteins and DNA concentrations were measured in parotid gland during chronic heat exposure (34 degrees C). Subcellular fractionation was performed. Exposure to heat resulted in a decrease in gland size and in DNA concentration; amylase activity/100 mg tissue and amylase specific ac...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90059-6
更新日期：1983-01-01 00:00:00
abstract：:The aim of this study was to investigate the potential effects of vitamin K1 supplementation on skeletal changes related to fluoride in growing rats. Forty male Wistar rats aged 4 weeks were assigned at random into three groups: high-dose fluoride (125 ppm) group; high-dose fluoride + vitamin K1 (0.2 mg/(g day)) group...
journal_title：Archives of oral biology
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1016/j.archoralbio.2009.02.001
更新日期：2009-06-01 00:00:00
abstract：:The scattering phase functions for HeNe-laser light of dentine sections 10-20 micron(s) thick were measured. The functions perpendicular to the tubules had first-order maxima at angles of 4 degrees for bovine dentine and 5 degrees for human dentine; those parallel to the tubules showed no first-order maxima. Several c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90098-f
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:Gingival fibroblasts (GFs) can participate in the immune response and play an immune-regulatory role. This study was to evaluate whether GFs can be differently activated and polarized into distinct functional subtypes in response to T helper type 1 (Th1), or Th2 stimuli. DESIGN:Human GFs were stimulated by t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.04.019
更新日期：2019-06-01 00:00:00
abstract：:Ureaplasmas were detected in the saliva of 3.5 per cent (12/342) of subjects. A total of 60 isolated strains did not revert to bacterial forms on repeated subculture in antibacterial agent-free media, failed to grow in serum-free media, were inhibited by digitonin and hydrolysed urea but not glucose or arginine. Twent...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90188-7
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVES:The antibacterial activity of Coffea canephora extract was evaluated in vitro against Streptococcus mutans and Streptococcus sobrinus. The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and time-kill assays. The effect of the extract on dental demineralisation was also inves...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.12.001
更新日期：2011-06-01 00:00:00
abstract：OBJECTIVES:To assess the association between the promoter region of serotonin transporter (5-HTTLPR) gene polymorphism and recurrent aphthous stomatitis (RAS) occurrence. DESIGNS:We performed the meta-analysis to determine the potential association between 5-HTTLPR polymorphism and RAS. We retrieved revelant studies f...
journal_title：Archives of oral biology
pub_type： 杂志文章,meta分析
doi：10.1016/j.archoralbio.2019.104555
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. DESIGN:Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.09.005
更新日期：2012-04-01 00:00:00
abstract：:Thirty-nine different microorganisms commonly found in supragingival plaque and salivary sediment were screened for their ability to raise the pH by producing base from arginine, lysylarginine and urea. Only Actinomyces naeslundii and Staphylococcus epidermidis showed significant pH-rise activity with all three compou...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90045-9
更新日期：1989-01-01 00:00:00
abstract：:This paper reports the development of a protocol allowing hybridization and detection of DNA fixed to nylon colony lifts from up to three species of bacteria simultaneously. Half ml samples of serial dilutions of pure cooked-meat broth (CMB) cultures of Capnocytophaga ochracea, Actinobacillus actinomycetemcomitans and...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90105-u
更新日期：1993-11-01 00:00:00
abstract：OBJECTIVE:Chondrocyte apoptosis is a pathological manifestation of osteoarthritis. The goal of this report was to explore the role of nitric oxide in chondrocyte apoptosis in osteoarthritic mandibular condylar cartilage. DESIGN:This study used our reported experimental unilateral anterior crossbite in vivo rat model a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.03.006
更新日期：2019-05-01 00:00:00
abstract：:Inhibitory jaw reflexes are believed to be important for protecting the teeth and temporo-mandibular structures from damage during sudden or forceful biting or mastication. Accordingly, alterations in these reflexes are sometimes implicated in aetiologies proposed for oro-facial pain syndromes, although the associatio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.02.012
更新日期：2011-09-01 00:00:00
abstract：:A task-force to resolve 26 pending forensic caseworks was carried out. We tested four different protocols to extract DNA from molar and pre-molar teeth from 26 cadavers with post-mortem intervals from 2 months to 12 years. We compared the amount of DNA and DNA profiles with the time elapsed between death and laborator...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.08.014
更新日期：2012-11-01 00:00:00
abstract：:Prednisolone (2 mg/kg) was injected daily for 14 days. Collagen and protein biosynthesis were measured in isolated molar and incisor pulps by the incorporation of [14C]-proline into protein and collagen in vitro. Collagen solubility, free proline content, prolyl hydroxylase activity, collagenolytic activity and DNA an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90153-x
更新日期：1983-01-01 00:00:00
abstract：:An appropriate model was created by the paraperiosteal injection of mustard oil (20% allyl isothiocyanate dissolved in mineral oil) into the periarticular temporomandibular tissue of anaesthetized rats. Inflammation was assessed by the plasma extravasation of Evans' blue dye bound to plasma protein. This was confirmed...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90026-5
更新日期：1992-01-01 00:00:00
abstract：OBJECTIVES:This study aimed to evaluate and compare the early biological effects of allogeneic bone marrow-derived mesenchymal stem cells (BMSCs) versus salmon calcitonin (SC) on healing of surgically induced mandibular bone defects in osteoporotic rats. METHODS:Sixty-one female albino rats were included in this study...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104934
更新日期：2020-12-01 00:00:00
abstract：:The rate of synthesis of secretory proteins increases significantly in rat parotid glands after stimulated discharge of stored proteins. How any difference in the amount of secretory protein discharge affects the rate of subsequent protein synthesis, and whether this post-secretory synthesis is regulated at the level ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90147-7
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:To compare the densities of CD1a + immature and CD83+ mature dendritic cells, and inflammatory infiltrate cells between smokers and non-smokers with oral leukoplakia. Parameters associated with malignant transformation were also evaluated. DESIGN:21 smokers and 23 non-smokers diagnosed with oral leukoplakia ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.07.008
更新日期：2018-11-01 00:00:00
abstract：:Green tea is a leading beverage in the Far East for thousands of years; it is regarded for a long time as a health product. Green tea is important source of polyphenol antioxidants. Polyphenols including epigallocatechin 3 gallate (EGCG) constitute the most interesting components in green tea leaves. Green tea has the...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2011.11.017
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:The loss of teeth not only causes damage to oral function but also is associated with cognitive impairment. Previous studies have reported that chewing can increase CBF, and CBF plays an important role in cognitive function. Whether the loss of teeth can lead to cognitive impairment by reducing CBF is unclear...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.05.004
更新日期：2019-06-01 00:00:00
abstract：:Pulp tissues isolated from rats treated with either colchicine (0.5, 0.75 or 1.0 mg/kg) or beta-lumicolchicine (1.0 mg/kg) for 24 h were incubated in Tyrode buffer. Prostaglandin (PG) I2 and thromboxane (TX) A2 released into the medium were determined by radioimmunoassay as their stable metabolites, 6-keto-PGF1 alpha ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90063-5
更新日期：1988-01-01 00:00:00
abstract：:It is known that pulpal fluid movement through dentinal tubules causes dentinal hypersensitivity and that pain can be reduced by decreasing the fluid flow. The aim of this study was to evaluate dentinal permeability and morphology after a single exposure to experimental phytocomplex substances containing oxalates. The...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.02.010
更新日期：2006-08-01 00:00:00
abstract：OBJECTIVE:Wnt5a is generally considered a non-canonical Wnt family member and plays an important role in the development of several tissues through regulation of cell fate, proliferation, migration, polarity and death. This study investigates its expression mode in human tooth development and the involved cell signal t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.12.002
更新日期：2010-02-01 00:00:00
abstract：:Herpes simplex virus type 1 (HSV1) can be rapidly identified in saliva from patients with acute herpetic gingivostomatitis, by in vitro amplification using the polymerase chain reaction and specific primers. Amplification of DNA results in a product of 110 bp length corresponding to the region 1381-1490 bp of the HSV1...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90113-m
更新日期：1992-10-01 00:00:00
abstract：:The expression of anchorage independence in malignant oral epithelial cells retrieved from colonies formed in agarose and tumours formed in athymic mice was examined. The original epithelial cell lines were derived from lingual and palatal squamous cell carcinomas induced in rats by the carcinogen 4-nitroquinoline N-o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90143-x
更新日期：1989-01-01 00:00:00
