Abstract：
:This paper reports the development of a protocol allowing hybridization and detection of DNA fixed to nylon colony lifts from up to three species of bacteria simultaneously. Half ml samples of serial dilutions of pure cooked-meat broth (CMB) cultures of Capnocytophaga ochracea, Actinobacillus actinomycetemcomitans and Prevotella intermedia were grown on trypticase soy blood agar (TSBA) plates for 7 days in an anaerobic chamber. From the same CMBs a further set of dilutions was completed that contained all three species. Samples from these dilutions produced mixed-growth TSBA plates following anaerobic incubation for 7 days. After incubation, colony counts on pure-growth TSBA plates were enumerated by colony counter. Colony counts of C. ochracea, A. actinomycetemcomitans and P. intermedia on mixed-growth TSBA plates were enumerated by nylon colony lift, simultaneous hybridization with non-isotopic whole chromosomal DNA probes and alkaline phosphatase substrates generating three colours. The results indicate that the protocol correctly identified and differentiated between the three species on mixed-growth TSBA plates. The proportions of each species and mean total colony count expected by counting pure plates were in agreement with the proportions of each species and total colony counts enumerated by DNA probes on mixed growth plates. The development of simultaneous hybridization and multicolour detection will result in improved data recovery from dental plaque samples, in addition to reducing the cost and labour required in current colony-lift protocols, without affecting the specificity or sensitivity of the probes used.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Cross DL,Ellender JA,Smith GLdoi
10.1016/0003-9969(93)90105-usubject
Has Abstractpub_date
1993-11-01 00:00:00pages
931-5issue
11eissn
0003-9969issn
1879-1506pii
0003-9969(93)90105-Ujournal_volume
38pub_type
杂志文章abstract：BACKGROUND:Variances in fibroblasts' α2β1 integrin intensity may lead to altered adhesion to type I collagen and consequently to suppression of phagocytosis which may be one of the mechanisms for drug induced gingival overgrowth. The present study aimed to evaluate the genotype and allele frequencies of α2 integrin +80...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.12.003
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:This critical review summarizes the current knowledge of the structural and functional characteristics of periodontal mechanoreceptors, and understands their role in the signal pathways and functional motor control. METHOD:A systematic review of the literature was conducted. Original articles were searched thr...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2017.02.010
更新日期：2017-06-01 00:00:00
abstract：:The scattering phase functions for HeNe-laser light of dentine sections 10-20 micron(s) thick were measured. The functions perpendicular to the tubules had first-order maxima at angles of 4 degrees for bovine dentine and 5 degrees for human dentine; those parallel to the tubules showed no first-order maxima. Several c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90098-f
更新日期：1991-01-01 00:00:00
abstract：:To investigate whether mechanical deformation of osteoblasts exerts an influence upon parathyroid hormone (PTH)-induced bone resorption, the effect of medium conditioned by a statistically stretched, osteoblast-enriched, bone cell population on PTH-induced osteoclast-like cell formation in mouse bone marrow cultures w...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/S0003-9969(97)00001-0
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to investigate the expression of pannexin3 (Panx3) in human odontoblast-like cells (hOBs) and its hemichannel function in mediating ATP release. METHODS:RT-PCR and immunofluorescence analysis were used to detect the expression of pannexins (Panxs) in human dental pulp tissue and cult...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.07.005
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:The influence of low-level fluoride (F) concentrations and lesion characteristics on the remineralisation of sub-surface root caries was investigated in vitro. DESIGN:Experimentally produced dentinal carious lesions were exposed to artificial saliva in the presence of 0.00ppm, 0.48ppm, 2.49ppm or 4.91ppm F (...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.08.001
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:This research investigates the ability of live and heat-killed (HK) Lactic Acid Bacteria (LAB) to interfere with Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 during biofilm formation. DESIGN:Eight Lactobacillus spp. and two oral colonizers, pathogenic Streptococcus mutans and resident ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.004
更新日期：2017-06-01 00:00:00
abstract：:Apoptosis in regressive primary (deciduous) dental primordia was examined in the embryos of Suncus murinus, which is a monophyodont. The primary tooth germs of S. murinus are temporarily formed and disappear during the embryonic period before they are calcified. Most primary tooth germs reach the bell stage and degene...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00007-3
更新日期：2001-07-01 00:00:00
abstract：:Chronic snuff dipping has been associated with oral cancer in man and experimental animals. Here, the effects of a water-extract of snuff on the in-vitro development of human lymphokine-activated killer (LAK) activity were examined. The snuff extract inhibited both LAK cytotoxicity and DNA synthesis in a dose-dependen...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90064-7
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:The existence of extra-pineal melatonin has been observed in various tissues. No prior studies of melatonin in human oral mucosal tissue under the condition of chronic inflammation have been reported. The aim of this study was to investigate the presence of melatonin in oral mucosal tissue of patients with or...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.007
更新日期：2017-06-01 00:00:00
abstract：:Small, quasi-rhythmical tremor of the jaw occurs at rest and during voluntary movements. In peripheral limbs, tremor consists of a component due to mechanical resonance properties of the system, and a neurogenic component mediated by a central pacemaker or neural loops. The present study attempted to determine if thes...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00091-5
更新日期：1998-01-01 00:00:00
abstract：OBJECTIVE:To investigate the potential effects of IFN-03A5 on the responsiveness of human gingival fibroblasts to bacterial challenge. DESIGN:mRNA and protein expression of CD14, TLR2 and TLR4 in human gingival fibroblasts was detected by quantitative polymerase chain reaction (Q-PCR) and flow cytometry. The effect of...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.10.005
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to investigate the relation between the remaining area of periodontal attachment and the attachment levels for each type of permanent teeth in a Chinese population by using micro-computed tomography (micro-CT) scans. DESIGN:A total of 440 extracted permanent teeth (including each t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.015
更新日期：2017-09-01 00:00:00
abstract：:In a previous study, it was shown that tooth germs of neonatal homozygous parathyroid hormone-related protein (PTHrP)-knockout mice are penetrated or compressed by the surrounding alveolar bone, suggesting an important role for PTHrP in the formation and activation of osteoclasts around growing tooth germs. In order t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00026-2
更新日期：2002-09-01 00:00:00
abstract：:Dental follicle has been implicated as the origin of alveolar bone, cementum and periodontal ligament, but there is no direct evidence of their cellular lineage. The present pilot study was designed to characterize the phenotype of cultured cells obtained from the dental follicle of neonatal mouse molars. Developing m...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00033-3
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVES:The aim of the present study was to investigate whether clinical (severe tooth loss) and subjective (chewing difficulties) indicators of oral health outcomes are associated with socioeconomic position and to explore the age-sex differences in the magnitude of the social gradient in Korea using data from the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.07.021
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:This study aimed to identify candidate genes as potential biomarkers in nasopharyngeal carcinoma (NPC) by bioinformatical analysis. METHODS:Three microarray datasets: GSE32906, GSE15170, GSE53819 were download from public database and analyzed to identify the differentially expressed genes (DEGs) between NPC...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.07.003
更新日期：2019-10-01 00:00:00
abstract：OBJECTIVE: Considering the higher rate of oral cancer, and reduction in salivary antioxidants in smokers as indicated in previous studies, antioxidant- containing nutrients such as green tea, seem to be beneficial in counteracting against oxidative stress in this group. This study assessed the salivary total antioxidan...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.002
更新日期：2017-06-01 00:00:00
abstract：:Unitary discharges of periodontal mechanosensitive (PM) fibres were recorded from cat inferior alveolar nerve. More than half (66%) of the PM units were single-tooth ones sensitive to only one tooth. The majority (78%) responded sustainedly to stimulation of the mandibular canine tooth. The multitooth units responded ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90036-1
更新日期：1994-01-01 00:00:00
abstract：OBJECTIVE:Increasing attention has been given to the potential protective roles of specific antioxidant nutrients found in fruits and vegetables. However, there are relatively few reports on the cancer chemopreventive effects of lycopene or tomato carotenoids in animal models. Therefore, the chemopreventive efficacy of...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.12.007
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:Replacement teeth in reptiles and mammals develop from a successional dental lamina. In monophyodont (single generation) species such as the mouse, no successional lamina develops. We have selected a reptilian monophyodont species - the Veiled Chameleon (Chamaeleo calyptratus) - to investigate whether this is...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.10.019
更新日期：2013-02-01 00:00:00
abstract：:Many studies have shown that various myosin isoforms are involved in muscle contraction. A search for specific antibodies directed against the myosin heavy chain (MHC) resulted in the identification of at least two main classes, referred to as MHC type I and type II. In this study, immunohistology and gel electrophore...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00092-3
更新日期：2000-12-01 00:00:00
abstract：OBJECTIVES:Tobacco use is one of the most important public health problems worldwide. It is also linked to impairment of normal immunologic surveillance and defence mechanism of polymorphonuclear leukocytes. Tobacco smoke and its components have been seen to affect the phagocytic ability and viability of polymorphonucl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.09.018
更新日期：2015-02-01 00:00:00
abstract：:Degradation of phenylalanine and leucine by resting cells of Eubacterium brachy ATCC 33089 were studied under strict anaerobic conditions. The effects of metronidazole and air on the metabolism were also studied. The organism principally produced phenylpropionate and isocaproate from phenylalanine and leucine, respect...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90080-9
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND:Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteoblast secretion of IL-6 plays an important role in the regulation of bone metabolism. Angiotensin II (Ang II) has been shown to regulate the expression of potent inflammatory factors, including MCP-1 and IL-6, by stimulating en...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.09.016
更新日期：2011-03-01 00:00:00
abstract：:The inability of fibrocartilage, specifically the temporomandibular joint (TMJ) disc, to regenerate and remodel following injury presents a unique problem for clinicians. Tissue engineering then offers a potential regenerative therapy. In vitro testing provides a valuable screening tool for potential tissue engineered...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2017.07.013
更新日期：2017-11-01 00:00:00
abstract：OBJECTIVES:A resin infiltrant was employed for the treatment of active white spot lesions due to its ability to penetrate into the enamel pores and prevent the progression of the lesion. However, limited information is available about its mechanical effect on different artificial enamel lesions as well as on its resist...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.07.011
更新日期：2018-11-01 00:00:00
abstract：:Transmission electron microscopy showed that when either dentinal phosphoprotein or calcium-treated phosphoprotein or phosvitin were introduced during type I collagen fibrillogenesis the fibrils formed were significantly wider and the cross-banding was more distinct than in the absence of phosphoprotein. The collagen ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90068-w
更新日期：1993-09-01 00:00:00
abstract：OBJECTIVES:brain-derived neurotrophic factor (BDNF), which is produced in rat submandibular gland, is one of the most abundant neurotrophins in the central nervous system. It is generally accepted that occlusal disharmony causes stress. The purpose of the present study was to investigate whether occlusal disharmony-ind...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.09.001
更新日期：2011-01-01 00:00:00
abstract：:Both Sjögren's syndrome and therapeutic irradiation for head and neck cancer lead to irreversible damage of the parenchyma of the salivary glands. This report describes an attempt to grow miniature pig (minipig) parotid gland cells on artificial films and tubular scaffolds with the ultimate intention of developing bio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.10.001
更新日期：2006-05-01 00:00:00
