当前位置： SCI文献检索 > ARCHIVES OF ORAL BIOLOGY期刊下所有文献 > Enhancement by conditioned medium of stretched calvarial bone cells of the osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures.

Enhancement by conditioned medium of stretched calvarial bone cells of the osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures.

Abstract：

:To investigate whether mechanical deformation of osteoblasts exerts an influence upon parathyroid hormone (PTH)-induced bone resorption, the effect of medium conditioned by a statistically stretched, osteoblast-enriched, bone cell population on PTH-induced osteoclast-like cell formation in mouse bone marrow cultures was examined. The conditioned medium of stretched cultures stimulated bone marrow cells to differentiate into tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells in the presence of 10(-8) M PTH(1-34). The stimulatory effect of the conditioned medium was significant when the bone cells were stretched at 2 mm deflection in the 90% subconfluent state. Conditioned medium from cultures in the 50% subconfluent state failed to enhance osteoclast-like cell formation. PTH-induced, TRAP-positive multinuclear cells were decreased in number by the addition of stretch-conditioned medium in the postconfluent state. When 10(-6) M indomethacin was added to bone cell cultures during stretching, the resulting conditioned medium suppressed PTH-induced, TRAP-positive multinuclear cell formation. However, even if 10(-8) M prostaglandin E2 was added to the stretched bone cell cultures along with the indomethacin, the resulting conditioned medium did not reverse the suppression of the PTH-induced, osteoclast-like cell formation. These findings suggest that bone resorption in response to continuous mechanical deformation is regulated by cells of the osteoblast lineage such as preosteoblasts, osteoblasts, bone lining cells and osteocytes in vivo, and that prostaglandins, but not prostaglandins E2, are involved in the stretch-enhanced, osteoclast-like cell formation.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Soma S,Matsumoto S,Takano-Yamamoto T

doi

10.1016/S0003-9969(97)00001-0

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

205-11

issue

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(97)00001-0

journal_volume

pub_type

杂志文章

在线工具

Enhancement by conditioned medium of stretched calvarial bone cells of the osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures.