Abstract：
OBJECTIVE:Gingival fibroblasts (GFs) can participate in the immune response and play an immune-regulatory role. This study was to evaluate whether GFs can be differently activated and polarized into distinct functional subtypes in response to T helper type 1 (Th1), or Th2 stimuli. DESIGN:Human GFs were stimulated by the Th1 cytokine, IFN-γ, and lipopolysaccharide (LPS), separately or in combination (Th1 stimuli), or by Th2 cytokines, IL-4 and IL-13, separately or in combination (Th2 stimuli). mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, IL-12), anti-inflammatory cytokines (IL-4, IL-10, IL-13), chemokines ((C-X-C motif chemokine (CXCL)10, CXCL11, C-C motif ligand 20), molecules involved in antigen presentation ((human leukocyte antigen (HLA)-ABC, HLA-DP, CD74, CD40)), LPS-recognizing components (Toll-like receptor 4, CD14), collagen type 1 (COL1), matrix metalloproteinase (MMP)-1, and inducible nitric oxide synthase (iNOS) and the protein levels of IL-6, CXCL11, CD14, and COL1 accumulated in supernatants were analyzed using real-time PCR and ELISA, respectively. The cell surface levels of the molecules involved in antigen presentation were detected by flow cytometry. RESULTS:Th1 stimuli strongly upregulated the expression of diverse cytokines, chemokines, molecules involved in antigen presentation, LPS-recognizing components, MMP-1, and iNOS, whereas Th1 stimuli inhibited the expression of COLI. In contrast, Th2 stimuli strongly upregulated the levels of COL1 and IL-4, while the expression levels of the other factors were minimally affected or even inhibited. CONCLUSION:These results show that GFs can be polarized into functionally distinct subtypes, immune-activating but tissue-destructive GF1 or tissue-reparative GF2, in response to Th1 and Th2 stimuli, respectively.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Jang JY,Baek KJ,Choi Y,Ji Sdoi
10.1016/j.archoralbio.2019.04.019subject
Has Abstractpub_date
2019-06-01 00:00:00pages
171-178eissn
0003-9969issn
1879-1506pii
S0003-9969(19)30051-2journal_volume
102pub_type
杂志文章abstract：:The distribution of substance P (SP)-containing synaptic terminals in the hypoglossal nucleus (XII) of adult rats was examined by retrograde peroxidase labelling and immunocytochemistry. From the location of peroxidase injections into the tongue and of labelled neurones in the ventral lamina of XII, motor neurones tha...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00040-0
更新日期：1999-08-01 00:00:00
abstract：:A series of unstimulated and stimulated saliva samples from healthy volunteers was tested for EGF by radioimmunoassay. Salivary EGF, like other salivary constituents, was secreted in a diurnal pattern. On stimulation the rate of EGF secretion doubled but the concentration fell by 70% because of dilution by salivary fl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90032-6
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:Despite the targeted and incidental exposure of the oral cavity to probiotic bacteria, relatively little information is available concerning their bacteriological effects or ecological fate in this environment. We have investigated the effects of an oral probiotic bacterium, Lactobacillus reuteri on salivary ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.04.004
更新日期：2011-11-01 00:00:00
abstract：:Recent reports characterizing the physiological properties of normal human labial acini are reviewed with particular emphasis on mechanisms related to fluid secretion. In contrast to the salivary glands of several experimental animals, human labial acinar cells do not appear to have a1-adrenergic receptors, substance ...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/s0003-9969(99)00044-8
更新日期：1999-05-01 00:00:00
abstract：OBJECTIVES:Chronic injury in tongue causes the variety of reactions in the oral cavity, frequently leading to its functional and structural disintegrity including inflammation and sensory dysfunction, but its detailed profiles were not elucidated yet. One of the chronically injured tongue such as tongue piercing, as a ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.12.008
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVES:Knowledge of the various possible morphologies and courses of the inferior alveolar nerve and artery is important for successful and safe surgical procedures in the mandibular region. The purpose of this study was to verify the histologic features and fascicular arrangement of the inferior alveolar nerve and...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.09.007
更新日期：2015-12-01 00:00:00
abstract：:Small GTPase Rab27 has been implicated in the regulation of different types of membrane trafficking, including melanosome transport and various regulated secretion events. We have previously shown that Rab27 and its effectors, Slac2-c/MyRIP and Slp4-a/granuphilin-a, are involved in the control of isoproterenol (IPR)-i...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.12.007
更新日期：2009-04-01 00:00:00
abstract：:Evidence was sought for urea-induced pH gradients in dental plaque microcosm biofilms cultured from the mixed salivary bacteria in a multi plaque 'artificial mouth'. Application of 500 mmol/l urea for short periods (6 min) to 5-8 mm maximum-thickness plaques induced intraplaque pH gradients of up to 0.7 pH units with ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90147-3
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the association between polymorphisms in DLX1, DLX2, MMP13, TIMP1 and TIMP2 genes with dental fluorosis (DF) phenotype. DESIGN:Four hundred and eighty one subjects (108 with DF and 373 DF free) from 6 to 18 years of age were recruited. This population lived in Rio de Janeiro, a city with fluorida...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.01.009
更新日期：2017-04-01 00:00:00
abstract：:The forces that develop in the human temporomandibular joint during function have never been directly measured, yet many patients exhibit excessive localized wear and tear of the joint, suggesting that at times these forces exceed tissue tolerance. The purpose here was to gain insight into vibration transmission betwe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00068-1
更新日期：2001-11-01 00:00:00
abstract：AIM:To compare the craniocervical angles and distances between temporomandibular dysfunction (TMD) and free TMD subjects. CASUISTIC AND METHODS:The sample consisted of young adults, of both genders, with age ranging between 18 and 30 years. TMD diagnosis was based on the clinical criteria of the Research Diagnostic Cr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.06.002
更新日期：2010-09-01 00:00:00
abstract：OBJECTIVES:The aim of the study was to investigate differences in the shape of the palatal rugae in two populations of India and develop discriminant function to identify the populations based on rugae shape. METHODS:Thirty plaster casts from each population group, equally distributed between the sexes and belonging t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.04.006
更新日期：2007-10-01 00:00:00
abstract：:The influence of indigenous Streptococcus mutans on naturally-occurring levels of IgA antibodies was studied in 42 lactating females. Breast milk, parotid and whole-saliva samples were collected and analysed by the ELISA method for IgA antibodies, reacting with antigens from Strep. mutans. All salivas and breast milk ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90149-x
更新日期：1987-01-01 00:00:00
abstract：:Whether the motor programme executed by the digastric muscles during a forceful bite is modified according to a subject's expectation that the resistance between the teeth will change was investigated. There were two experimental conditions: (1) tracking a ramp (drawn on an oscilloscope screen) by biting (isometricall...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90195-r
更新日期：1993-02-01 00:00:00
abstract：:Electromyographic recordings (EMG) obtained from intramuscular electrodes inserted in the lateral pterygoid muscle (lat. pt.) of 29 juvenile and adolescent monkeys were assessed in relation to the recorded activity of three jaw elevating muscles, temporalis, masseter and medial pterygoid, and a jaw muscle depressor, t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90087-5
更新日期：1982-01-01 00:00:00
abstract：:The peroxidase activity in human whole saliva is due to salivary peroxidase and, in some cases, myeloperoxidase; it is usually determined by spectrophotometric methods based on the rate of oxidation of chromogen substrates. Thiocyanate ion, a normal component of saliva, interferes with these kinetic assays by competin...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90095-6
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVE:To identify the genetic risk markers of aggressive periodontitis (AgP), researchers focus on genetic components that regulate the immune response. Therefore the purpose of this study was to investigate genetic impact of monocyte chemoattractant protein (MCP)-1-2518A/G and CC chemokine receptor 2 (CCR2) -190G/...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.016
更新日期：2017-08-01 00:00:00
abstract：:Human epithelial cells isolated from adult gingival and infant palatal biopsies were cultured using 3T3 feeder cells. The colony-forming efficiency was about 0.8 per cent with cholera toxin and epidermal growth factor (EGF). The cell yield of cultures from infant palates depended on the concentration of cholera toxin ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90113-9
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVES:This study evaluated the erosive tooth wear promoted by commercial whitening toothpastes on eroded dentin in vitro. DESIGN:Ninety bovine roots were embedded, polished and subjected to the baseline profile analysis. The samples were protected in 2/3 of the dentin surface and were randomly assigned to 6 group...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.104580
更新日期：2020-01-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of experimentally created disordered occlusion on the mandibular condylar cartilage in terms of histological morphology and expression of MMP-3, MMP-9, TIMP-1 and aggrecan. MATERIALS AND METHODS:Eighty 8-week-old Sprague-Dawley rats were randomly divided into two experimental (Exp)...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.07.013
更新日期：2010-11-01 00:00:00
abstract：:Two mechanisms that contribute to tooth eruption are proposed from analysis of the human jaws as elastic structures which are deformed intermittently under functional occlusal loads. One possible source of eruptive force is the transient pressure gradient established within each jaw as it is deformed. The second sourc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90093-8
更新日期：1987-01-01 00:00:00
abstract：:The release studied by incubating tissue slices in an enriched, oxygenated medium in the presence and absence of secretagogues indicated that slices from 1-day-old rats released K+ in response to carbamylcholine, but not in response to epinephrine. The response to carbamylcholine was dose-related, with a 12.6 +/- 1.3 ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90100-5
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVES:The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. DESIGN:We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal days 1-100 by ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.09.007
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVE:The present study was designed to investigate (1) whether the non-human primate would be an appropriate animal model for the study of spontaneous periodontitis and its association with metabolic syndrome (MetS), and (2) whether microRNAs (miRNAs) play roles in the co-development of metabolic disorders and per...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.01.004
更新日期：2014-04-01 00:00:00
abstract：:A large earthquake (8-9 on the Richter scale) and a series of aftershocks took place on 2 March 1985 in Santiago, Chile. The characteristics of over 22,000 births registered in three public hospitals in the same year were reviewed. A significant increase in the rate of facial clefts was found; 2.01 per 1000 births in ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)00146-3
更新日期：1995-01-01 00:00:00
abstract：:In the complex neurovascular control of the orofacial area, the only vasoconstrictor action is mediated by the sympathetic nervous system; however, its functional role is still unclear as little evidence exists of vasoconstrictor responses to physiological stimuli in both animal and human models. Aim of the present st...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.01.003
更新日期：2009-04-01 00:00:00
abstract：:Samples of dentine from normal deciduous and permanent teeth were demineralized with EDTA and cleaved with CNBr. The resulting peptides were separated by means of electrophoresis on polyacrylamide slab gels, stained with Coomassie blue and examined by transmitted light. Final identification was performed by means of a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90124-9
更新日期：1984-01-01 00:00:00
abstract：:Osteoclastogenesis is the major cellular event responsible for bone loss and is triggered by inflammation. Acai-berry has proven anti-inflammatory effects. However, there is a lack of evidence for its effects on osteoclastogenesis. Thus, the aim of this study was to determine whether acai-berry extract (ABE) could inh...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.03.016
更新日期：2016-08-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Epithelial-mesenchymal transition (EMT) has been proved to occur in drug-induced gingival overgrowth. However, the specific pathogenic mechanism remains uncertain. The aim of this study is to examine the expression of EMT markers in cyclosporine A (CsA)-induced gingival overgrowth in rat models...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.024
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of a fluoride toothpaste containing nano-sized sodium trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. DESIGN:This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.09.019
更新日期：2018-12-01 00:00:00
