Cloning and expression of a Porphyromonas (Bacteroides) gingivalis protease gene in Escherichia coli.

Abstract:

:A genomic library of Bacteroides gingivalis W83 chromosomal DNA was constructed in the Escherichia coli lambda (lambda) vector EMBL 4. Three recombinant lambda phages expressing a cloned protease were identified in the library. All three lambda phages contained cloned overlapping DNA fragments from the same region of the chromosome and encoded the same cloned protease. The cloned protease was expressed poorly using its own promoter in E. coli.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Arnott MA,Rigg G,Shah H,Williams D,Wallace A,Roberts IS

doi

10.1016/0003-9969(90)90137-y

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

97S-99S

eissn

0003-9969

issn

1879-1506

journal_volume

35 Suppl

pub_type

杂志文章
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  • New animal model of extrinsic dental erosion-Erosive effect on the mouse molar teeth.

    abstract:OBJECTIVE:Consumption of acidic food and drinks is considered as important risk factor for development of dental erosion. There are several in vitro and in situ studies focusing on the risk indicators and preventive treatment, however, the need for a standardized animal model has been emphasised for many years. The aim...

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  • Expression of Wnt5a in tooth germs and the related signal transduction analysis.

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    更新日期:2010-02-01 00:00:00

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    更新日期:1989-01-01 00:00:00

  • The effect of honey on human tooth enamel in vitro observed by electron microscopy and microhardness measurements.

    abstract::Various fruit juices with relatively low pH are known to have erosive effects on human tooth enamel in a reasonably short time. Honey, also with a relatively low pH, could do the same, but scanning electron microscopy showed no erosion of enamel by honey over a period of 30 min, neither did Knoop microhardness tests s...

    journal_title:Archives of oral biology

    pub_type: 杂志文章

    doi:10.1016/0003-9969(94)90110-4

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  • Delayed dental development in children with isolated cleft lip and palate.

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    pub_type: 杂志文章

    doi:10.1016/j.archoralbio.2006.08.002

    authors: Yigla M,Berkovich Y,Nagler RM

    更新日期:2007-01-01 00:00:00

  • Gene expression of markers associated with proliferation and differentiation in human keratinocytes cultured from epidermis and from buccal mucosa.

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    更新日期:1995-09-01 00:00:00

  • The surface structure of the epithelium of the hamster cheek pouch.

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    pub_type: 杂志文章

    doi:10.1016/0003-9969(82)90185-6

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    更新日期:1982-01-01 00:00:00

  • Protease activity in the mouse dental follicle during tooth eruption.

    abstract::Eruption results in the movement of the tooth from an intraosseous to a functional position in the mouth. This physiological movement occurs through a path of least resistance and is mediated by the dental follicle. In this study the presence of a 80-kDa proteinase of the gelatinase family in the dental follicle of ma...

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    pub_type: 杂志文章

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    authors: Shroff B,Norris K,Pileggi R

    更新日期:1995-04-01 00:00:00

  • Immunohistochemical localization of components of the insulin-like growth factor system in human permanent teeth.

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  • Partial pulp resection increases unimpeded eruption rates of the rat incisor.

    abstract::Thirty rats were divided into 3 equal groups. Following 9 days of induced unimpeded eruption, a single partial pulp resection was performed on the repeatedly-shortened lower left incisor of one group. A total of 3 resections, each repeated after 48 h, was performed on the animals in a second group. The lower left inci...

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    pub_type: 杂志文章

    doi:10.1016/0003-9969(83)90127-9

    authors: Pitaru S,Oelgisser D,Weinreb MM

    更新日期:1983-01-01 00:00:00

  • Orientation of occlusal contacts in the chimpanzee, Pan troglodytes verus, deduced from scanning electron microscopic analysis of dental microwear patterns.

    abstract::Analysis of dental microwear may be used to detect species differences in diet and to secure information about occlusal relationships and biomechanics of the jaw. Microscratch-orientation data evaluated with reference to several hypotheses about functional, wear-related, sex and age influences on occlusal contacts ind...

    journal_title:Archives of oral biology

    pub_type: 杂志文章

    doi:10.1016/0003-9969(84)90007-4

    authors: Gordon KD

    更新日期:1984-01-01 00:00:00

  • Phenotype and behaviour of dental pulp cells during expansion culture.

    abstract:OBJECTIVE:Primary pulp cell cultures are frequently used to study cellular responses, odontogenic potential and stem cell responses. Their isolation and expansion via a range of technical approaches are widely reported. The purpose of this study was to investigate the influence of isolation approach and extended expans...

    journal_title:Archives of oral biology

    pub_type: 杂志文章

    doi:10.1016/j.archoralbio.2009.06.008

    authors: Patel M,Smith AJ,Sloan AJ,Smith G,Cooper PR

    更新日期:2009-10-01 00:00:00

  • In vitro evaluation of different remineralization periods in improving the resistance of previously eroded bovine dentine against tooth-brushing abrasion.

    abstract::One dentine specimen was prepared from each of 90 bovine incisors. The samples were then evenly distributed among nine groups (A-I) and submitted to 10 alternating de- and re-mineralization cycles, including abrasion by tooth brushing. Each cycle started with a demineralization using the erosive soft drink Sprite Ligh...

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    更新日期:2001-09-01 00:00:00

  • Relationship of masseter electromyographic activity to mandible position in the freely-moving rat.

    abstract::Simultaneous records of masseter-EMG activity and position of the mandible were made on 4 Wistar rats eating four food types: standard food pellets (large pellets), 45 mg precision food pellets (small pellets), bread and pudding. Masseter activity was recorded with chronic bipolar silver EMG electrodes placed within t...

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    pub_type: 杂志文章

    doi:10.1016/0003-9969(83)90060-2

    authors: Thomas NR,Peyton SC

    更新日期:1983-01-01 00:00:00

  • The immunohistochemical localization of signal-transduction pathway components Jak1, Jak2, Jak3, Tyk2 and STAT-1 during early enamel and dentine formation in rat molars.

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    pub_type: 杂志文章

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  • Fluoride distribution and histological structure of human cementum.

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    更新日期:1988-01-01 00:00:00

  • Optimization of poly (lactic-co-glycolic acid)-bioactive glass composite scaffold for bone tissue engineering using stem cells from human exfoliated deciduous teeth.

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  • Transplantation of enzyme-treated teeth in the monkey Macaca fascicularis.

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    doi:10.1016/0003-9969(82)90128-5

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    更新日期:1982-01-01 00:00:00

  • Secondary ion mass spectrometry and X-ray microanalysis of hypomineralized enamel in human permanent first molars.

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  • Dimensions of the temporal glenoid fossa and tooth wear in prehistoric human skeletons.

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