Abstract:
:Normal keratinocytes from epidermis and from buccal mucosa underwent dissimilar stages of differentiation in the same culture medium and responded differently to changes in the composition of the medium. Manifestations of these variations were examined in terms of the expression at the mRNA level (as measured by reverse transcriptase-polymerase chain reaction) of three regulatory genes (cdc2, c-myc, and p53) and five that encode structural proteins (keratins K5, K10 and K13, involucrin, and filaggrin), in three growth-medium formulations. The culture conditions enhanced or retarded maturation; the observed alterations in gene expression correlated with these changes. Except for the proliferation genes, the non-keratinizing buccal mucosa generally responded more weakly than the orthokeratotic epidermis to culture-medium supplementation favouring differentiation. Gene expression in cultured keratinocytes reflected their ability to differentiate in vivo; genes were expressed even when the corresponding protein was not seen in vitro. Although keratin K10 is not prevalent in the buccal mucosa nor keratin K13 in the epidermis, the genes for both were found to be expressed in both tissues.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Brysk MM,Arany I,Brysk H,Chen SH,Calhoun KH,Tyring SKdoi
10.1016/0003-9969(95)00046-rsubject
Has Abstractpub_date
1995-09-01 00:00:00pages
855-62issue
9eissn
0003-9969issn
1879-1506pii
0003-9969(95)00046-Rjournal_volume
40pub_type
杂志文章abstract::The fragmentation of foods (breakage function) was measured in five humans on "bagged' single particles of 28 foods from three food groups. The change in the square root of the specific surface of the particles (the specific surface being the area of particle silhouettes, measured by image analysis, divided by origina...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00102-1
更新日期:1997-01-01 00:00:00
abstract::Parotid salivation was stimulated by infusion of bethanechol chloride into anaesthetized brushtail possums to ascertain maximal flow rates, salivary composition and dietary adaptations of salivary function. Secretion rates for one gland ranged from 5.3 +/- 0.16 to 84.3 +/- 3.20 microliters/min (2.4 +/- 0.07 to 37.8 +/...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90127-9
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVE:This study reports the expression of proinflammatory cytokines in temporomandibular joint (TMJ) of patients affected with temporomandibular osteoarthritis (OA). DESIGN:In twelve OA of the TMJ (OA-TMJ) affected patients and in six healthy volunteer subjects studied as control, the expression of IL1beta (inter...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2008.04.004
更新日期:2008-10-01 00:00:00
abstract::The response to stimulation of the parasympathetic innervation to parotid or submandibular glands of reserpinized rats was altered from that of untreated rats. Thus, acute reserpinization, like other types of sympathectomy, resulted in increase in volume of parasympathetically-evoked parotid or submandibular saliva wh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90040-2
更新日期:1984-01-01 00:00:00
abstract::The effects of ligation on body weight, protease activity and hair kynurenine content were observed. The body weights of parotid gland(PT)-ligated and of combined submandibular (SM)-, sublingual (SL)- and PT-ligated rats were much less than those of sham-operated controls. However, the weights of SM,SL-ligated rats we...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90137-9
更新日期:1988-01-01 00:00:00
abstract::Mandibular first-molar tooth germs were dissected from 16-day-embryo and new-born CD1 mice. By incubation in collagenase they were separated into enamel organ, papilla and follicle. Dental pulp was obtained from mandibular first molars of 3-, 7- and 10-day-old mice. Various combinations of epithelial and mesenchymal t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90022-7
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:To evaluate in vitro the effectiveness of different calcium-silicate (CS) cements specifically designed for dentine permeability reduction. DESIGN:Fifty dentine discs were prepared from caries free human molars extracted for surgical reasons. The treatment consisted in applying two different experimental CS ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.02.024
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVE:This study aimed to examine and compare the anti-caries effects of citrus lemon oil (CLO) and limonene in rats. METHODS:The minimal inhibitory concentrations of CLO and limonene were measured using the disk diffusion method. The rats were infected with Streptococcus sobrinus and assigned into four groups: (1...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104851
更新日期:2020-10-01 00:00:00
abstract::Masticatory muscle electromyograms (EMGs) were recorded while patients with bulbar myasthenia gravis chewed artificial food and compared with those of patients with ocular myasthenia gravis, patients in clinical remission who had previously suffered from bulbar myasthenia gravis and healthy individuals. Masticatory pe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00014-6
更新日期:2002-05-01 00:00:00
abstract::Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00160-7
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:Orofacial clefts result from failures of developing embryonic facial and palatal processes to either completely merge or fuse. Normal development of the facial primordia requires remodelling of the extracellular matrix, which is mediated in part by the matrix metalloproteinases (MMPs). MMPs can be considered ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.04.005
更新日期:2007-10-01 00:00:00
abstract::Soluble reduced lysozyme was extensively digested by a trypsin-like protease purified from the culture supernatant of the bacterium. The digestion peptides were separated and purified by reversed-phase high-performance liquid chromatography, and were subjected to amino acid analysis. The fragments were identified by t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90149-0
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. DESIGN:Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.09.005
更新日期:2012-04-01 00:00:00
abstract:OBJECTIVE:Increasing attention has been given to the potential protective roles of specific antioxidant nutrients found in fruits and vegetables. However, there are relatively few reports on the cancer chemopreventive effects of lycopene or tomato carotenoids in animal models. Therefore, the chemopreventive efficacy of...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.12.007
更新日期:2011-07-01 00:00:00
abstract::An immunofluorescence study of the expression of keratin 14 (K14) during the formation of filiform papillae was performed and the progress of keratinization of the epithelium of the rat tongue was monitored on semi-ultrathin sections by laser-scanning microscopy. Differential interference contrast (DIC) images were al...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(03)00118-3
更新日期:2003-08-01 00:00:00
abstract::The 10,000 g supernatant of wax-stimulated whole saliva was fractionated by gel filtration and its components were tested along with amino acids, small peptides and urea for their ability to stimulate this oxygen uptake, and for their effects on pH. Fractions containing the larger components, the proteins and large pe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90176-b
更新日期:1990-01-01 00:00:00
abstract::External apical root resorption (EARR) induced by orthodontic treatment and chronic periodontitis (CP) are complex phenotypes dependent on the interaction of multiple genetic and non-genetic risk factors. Apart from different environmental triggers, these phenotypes are caused by antagonistic biological mechanisms inv...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2018.08.006
更新日期:2018-11-01 00:00:00
abstract::Iron-deficiency anaemia was induced in hamsters by feeding a low-iron diet coupled with weekly bleeding. Respiration, assessed by measuring oxygen uptake, was significantly reduced (p less than 0.002) in cheek-pouch epithelia from iron-deficient animals. To relate data on oxygen consumption to cell numbers, the cell d...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90150-5
更新日期:1985-01-01 00:00:00
abstract::Dialysed extracts from dental plaque and a number of species of oral streptococci and actinomycetes have been investigated for their effects on cell growth, [3H]-thymidine uptake and 51Cr release from HeLa cell cultures. Eight of the 15 extracts inhibited cell growth and all but one inhibited [3H]-thymidine incorporat...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90178-9
更新日期:1982-01-01 00:00:00
abstract::Smears of buccal mucosa, dorsal surface of the tongue and floor of mouth were taken from 10 patients with histologically confirmed oral lichen planus and 12 healthy age- and sex-matched controls. In buccal smears, no significant differences in cytoplasmic and nuclear areas were observed between lesional, adjacent non-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00106-9
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteoblast secretion of IL-6 plays an important role in the regulation of bone metabolism. Angiotensin II (Ang II) has been shown to regulate the expression of potent inflammatory factors, including MCP-1 and IL-6, by stimulating en...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.09.016
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:Solobacterium moorei is a Gram positive bacterium that has been specifically associated with halitosis. The aim of this study was to characterize volatile sulfur compound (VSC) production by S. moorei. METHODS:S. moorei was either grown or incubated in the presence of various supplements prior to determining...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.09.011
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:The complex movement of the temporomandibular joint (TMJ) disc during mastication is controlled in large part by the disc's attachments to the surrounding tissues. This study seeks to address the lack of available quantitative data characterizing the extracellular matrix composition of the discal attachments ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.10.004
更新日期:2012-06-01 00:00:00
abstract::This study examined the expression of MMP-2 and MMP-9 in normal and human malignant oral keratinocytes. The expression of pro-MMP-2 and pro-MMP-9 was heterogeneous in the malignant cell lines. Normal oral keratinocytes expressed less pro-MMP-2 and more pro-MMP-9 than their malignant counterparts. Cells that expressed ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(03)00172-9
更新日期:2003-11-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to determine the influence of masticatory function, dental caries and socioeconomic status on the body mass index (BMI) of preschool children. METHODS:A cross-sectional study was conducted with a sample of 285 children aged three to five years allocated to three groups based o...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.032
更新日期:2017-09-01 00:00:00
abstract::Nerve fibres showing CGRP-immunoreactivity were exclusively distributed around blood vessels in the periodontal ligament of normal teeth. The CGRP-positive nerves as well as the vessels were more dense during tooth movement and reached a maximum density and intensity of immunoreaction after 3 days. After that, the imm...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90023-n
更新日期:1991-01-01 00:00:00
abstract::The structure of human dentine affected by familial hypophosphataemic rickets was investigated by polarized light and electron microscopy. Within the calcospherites both radially-orientated and collagen-orientated crystals were present, in approximately equal numbers. This was so, but less distinctly, in the calcosphe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90030-4
更新日期:1983-01-01 00:00:00
abstract::It is not known whether the long-term use of prosthetic or orthodontic appliances, which may be associated with fungal infections, affects Langerhans cells (LC) in the underlying mucosa. The number and distribution of LC was studied in rat palatal epithelium over a 7-day period after insertion of cover plates. Smears ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90112-x
更新日期:1989-01-01 00:00:00
abstract::Four-five months after the induction of diabetes, salivary tissues of male Wistar rats were preserved by glutaraldehyde fixation or rapid freezing in dry-ice cooled hexane. Fixed tissues were either processed and embedded for light and electron microscopy, or frozen and, together with unfixed tissues, sectioned and st...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90021-x
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVE:Respiratory epithelial cells are the first natural barrier against bacteria and viruses; hence, the interactions among epithelial cells, bacteria, and viruses are associated with disease occurrence and development. The effect of co-infection by P. gingivalis and influenza A virus (IAV) on respiratory epitheli...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.04.003
更新日期:2018-11-01 00:00:00