Abstract：
:This study examined the expression of MMP-2 and MMP-9 in normal and human malignant oral keratinocytes. The expression of pro-MMP-2 and pro-MMP-9 was heterogeneous in the malignant cell lines. Normal oral keratinocytes expressed less pro-MMP-2 and more pro-MMP-9 than their malignant counterparts. Cells that expressed high levels of both MMP-2 and MMP-9 showed the greatest degree of invasion through Matrigel in vitro compared to cells with either low or variable levels of these enzymes; normal keratinocytes were non-invasive in these conditions. The degree to which the cells invaded through Matrigel was similar to their motility in the absence of Matrigel and was not influenced by the activation of the pro-enzymes or the inhibition of enzyme activity using a chemical inhibitor of gelatinases. Cells were transplanted orthotopically to athymic mice and demonstrated a variable capacity not only to form tumours at the site of inoculation but, also, to metastasise; normal oral keratinocytes were non-tumorigenic. There was no correlation between the expression of either MMP-2 or MMP-9 and the tumorigenic/metastatic phenotype. The results emphasise the limitations of correlating in vitro and in vivo assays of tumour cell behaviour and suggest that invasion/motility in vitro may be a distinct phenotype from tumorigenicity/metastasis in vivo.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Robinson CM,Stone AM,Shields JD,Huntley S,Paterson IC,Prime SSdoi
10.1016/s0003-9969(03)00172-9subject
Has Abstractpub_date
2003-11-01 00:00:00pages
779-86issue
11eissn
0003-9969issn
1879-1506pii
S0003996903001729journal_volume
48pub_type
杂志文章abstract：OBJECTIVE:To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. DESIGN:Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.09.005
更新日期：2012-04-01 00:00:00
abstract：:The presence of human STRO-1(+)/CD146(+) periodontal ligament (PDL) cells has been reported, but obtaining a large amount of these cells is difficult. The purpose of this study was to evaluate the percentages of STRO-1(+)/CD146(+) cells in PDL cells and determine the effects of FGF-2 on the proliferation and multiline...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.12.003
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:The present study was designed to examine how oestrogen regulates proliferation, osteoblastic differentiation, collagen synthesis and periostin gene expression in primary human periodontal ligament (hPDL) cells. DESIGN:The short interfering RNA (siRNA) technique was used to inhibit oestrogen receptor beta (E...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.11.001
更新日期：2011-05-01 00:00:00
abstract：:Previous studies using potassium ion-selective microelectrodes have demonstrated that potassium concentrations in dental plaque fluid obtained by centrifugation are identical to whole plaque values determined immediately after collection. Such procedures were now used to examine the variations in potassium concentrati...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90030-1
更新日期：1988-01-01 00:00:00
abstract：:Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00160-7
更新日期：2003-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to assess the frequency of variations in tooth number, shape and position in the dental arch to determine if there are parts of the dental arch that show a higher prevalence of tooth variability. DESIGN:198 randomly selected Caucasian subjects (93 females, 105 males) aged between 10...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.11.009
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVES:This study evaluated the level and mechanism of apoptosis in human gingival fibroblasts (HGF) and murine fibroblasts (NIH/3T3) treated with a titanium tetrafluoride (TiF4) varnish compared those treated with a sodium fluoride (NaF) varnish. METHODS:Cells were treated with a TiF4, NaF (both 2.45%F) or placeb...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.10.039
更新日期：2019-01-01 00:00:00
abstract：:Four-five months after the induction of diabetes, salivary tissues of male Wistar rats were preserved by glutaraldehyde fixation or rapid freezing in dry-ice cooled hexane. Fixed tissues were either processed and embedded for light and electron microscopy, or frozen and, together with unfixed tissues, sectioned and st...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90021-x
更新日期：1986-01-01 00:00:00
abstract：IMPORTANCE:Notch proteins are cell surface transmembrane spanning receptors which mediate critically important cellular functions through direct cell-cell contact. Interactions between Notch receptors and their ligands regulate cell fate decisions such differentiation, proliferation and apoptosis in numerous tissues. W...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.04.003
更新日期：2014-07-01 00:00:00
abstract：:Periodontal bone loss was induced in mice of different genotypes by oral inoculation with Actinomyces viscosus. Relatively high bone loss occurred in the mutant X-linked immune deficiency, implicating the humoral immune system. Maximum bone loss was always associated with inoculations of 1.6 x 10(7) colony forming uni...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90013-1
更新日期：1988-01-01 00:00:00
abstract：:Neurophysiological exploration of the trigeminal sensory complex was done on 42 cats under ketamine anaesthesia, paying special attention to units receiving a periodontal input. Among 492 cells recorded in the trigeminal sensory complex, 73 responded to mechanical stimulation of the periodontium and were precisely loc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90138-3
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVE:The present study evaluated the nociceptive response induced by dentin hypersensitivity after dental erosion in rats that were exhibited to unpredictable chronic mild stress (UCMS)-induced depressive-like behavior. DESIGN:Adult male rats were subjected to UCMS (depression [D] group) or not (no depression [ND...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.005
更新日期：2017-08-01 00:00:00
abstract：:The application of detergent solutions to dissociated molar dental papillae of mice permitted precise observation of the extracellular fibrillar material in the odontoblast layer and the predentine. Two types of fibrils were identified: cross-banded fibrils of variable diameter (25-200 nm) and aperiodic microfibrils o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90092-1
更新日期：1988-01-01 00:00:00
abstract：:After single, oral doses, 8 h profiles of fluoride (F) concentrations in plasma were determined in healthy human volunteers. Bioavailability of F from bone-meal, calcium and CaF2 tablets was evaluated in relation to that of NaF. Tablets were administered either whole or as a finely-ground powder, either to fasting sub...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90074-4
更新日期：1987-01-01 00:00:00
abstract：:Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00051-9
更新日期：1996-08-01 00:00:00
abstract：INTRODUCTION:Angiogenesis is important in pulp-dentin formation. Among the regulatory factors, long noncoding RNA (LncRNA) is a class of functional RNA molecules that are not translated into protein and involved in regulating multiple physiological processes. The different expression of LncRNA and its target gene in de...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104691
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:The function of apoptosis and its regulation in odontoblasts remain unclear. In this study, we characterize the possible role of transforming growth factor (TGF)-beta 1 in the induction of apoptosis and the molecular mechanisms that mediate TGF-beta1-induced apoptosis in odontoblasts. METHODS:Annexin V/propi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.03.004
更新日期：2005-11-01 00:00:00
abstract：:To test the hypothesis that human tooth eruption requires a critical time period during which no force is applied to the erupting tooth, the eruption of a maxillary second premolar in the prefunctional stage was recorded during the afternoon or evening hours in eight children, using an optical device based on the prin...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00011-4
更新日期：1999-05-01 00:00:00
abstract：:Autoantibodies directed against nucleic acid and protein complexes present in cell nuclei characterize autoimmune diseases and are employed in diagnosis. The mechanisms by which these autoantigens escape immunological tolerance are largely unknown, but a number of recent observations suggest that modified self-protein...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00025-0
更新日期：2002-06-01 00:00:00
abstract：:A variable capacitance displacement transducer, previously employed for continuously monitoring movements of the rabbit mandibular incisor, was modified for use with the ferret mandibular canine. Comparing this tooth of limited growth with the tooth of continuous growth, similarities were seen in the reactions to extr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90177-2
更新日期：1983-01-01 00:00:00
abstract：:This study sought to determine if the diet-induced changes in submandibular salivary glands can be reversed. Two groups of male weanling Sprague-Dawley rats were fed purified diets containing 9% butter + 1% corn oil (group I, control) or 9% ethyl ester concentrate of n-3 fatty acids + 1% corn oil (group II, experiment...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90209-5
更新日期：1993-05-01 00:00:00
abstract：:The abrasiveness of food is a key determinant in the rate of physiological attrition (dental wear) in humans. With increasing food processing through time, the rate of physiological dental wear in human teeth has decreased markedly. Many consider such wear to be beneficial to oral health and that insufficient wear may...
journal_title：Archives of oral biology
pub_type： 历史文章,杂志文章
doi：10.1016/s0003-9969(99)00095-3
更新日期：1999-11-01 00:00:00
abstract：OBJECTIVE:To understand bone regeneration process after tooth extraction could be a clue to develop a new strategy for alveolar bone reconstruction. Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. In this study, we investigated the spat...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00153-5
更新日期：2003-10-01 00:00:00
abstract：OBJECTIVE:Root grooves are considered a risk factor for periodontal disease. The purpose of this study was to measure the length of the root of maxillary lateral incisors (MLI), as well as the width and depth of the concavities of their proximal surfaces, and also to determine the prevalence of palato-gingival grooves....
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.02.008
更新日期：2006-08-01 00:00:00
abstract：:Nicotine, a major component in tobacco smoke, stimulates the synthesis of prostaglandin E(2). We investigated the involvement of the transcription nuclear factor kappa B (NFkappaB) in the nicotine-induced expression of cyclooxygenase-2 (COX-2), a key enzyme for prostaglandin synthesis, in human gingival fibroblasts. N...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.11.006
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVE:This controlled split-mouth study aimed to estimate the effect of caries and related treatment on concentrations of interleukin (IL)-2, interferon (IFN)-γ, IL-12, IL-17A, IL-13, IL-10, IL-6, IL-5, IL-4, IL-22, tumor necrosis factor (TNF)-α, and IL1-β in gingival crevicular fluid (GCF) of caries affected teeth...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.104620
更新日期：2020-02-01 00:00:00
abstract：OBJECTIVE:The aim of the present study is to investigate the involvement of immunocompetent cells, pro-inflammatory cytokines and HSP, to evaluate a change of periodontal ligament during the initial stage of orthodontic tooth movement. DESIGN:In the present study, we investigated the distributional density of immunoco...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.11.010
更新日期：2011-05-01 00:00:00
abstract：:Small amalgam fillings were placed in maxillary and mandibular second molar and canine teeth for cine-radiographic analysis. The rosette strain gauges were bonded bilaterally to mandibular cortical bone below the second or third molars. The monkeys were placed in a restraining chair that did not restrict normal head, ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90139-1
更新日期：1986-01-01 00:00:00
abstract：:The fortuitous observation of salivary microliths in ferret was pursued in the present investigation. Major salivary glands obtained post-mortem from mature ferrets of either sex were examined with the use of histology and light microscopical histochemistry for calcium, protein, amino acids, mucosubstances and hydroly...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.07.009
更新日期：2006-03-01 00:00:00
abstract：OBJECTIVE:To assess a panel of risk factors associated to oral candidosis (OC) onset and its chronic maintenance by means of fuzzy logic (FL) approach and statistical traditional methodology (STM); to investigate their casual relationship within a multifactorial framework. DESIGN:Case-control study. SETTING:One hospi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.11.009
更新日期：2008-04-01 00:00:00
