Abstract：
OBJECTIVES:This study evaluated the level and mechanism of apoptosis in human gingival fibroblasts (HGF) and murine fibroblasts (NIH/3T3) treated with a titanium tetrafluoride (TiF4) varnish compared those treated with a sodium fluoride (NaF) varnish. METHODS:Cells were treated with a TiF4, NaF (both 2.45%F) or placebo varnish for 6 h and were then examined using the TUNEL method. The activities of caspase-3, -8 and -9 were assessed. cDNA for Bax, Bad, Bcl-2 and Fas-L was amplified by quantitative PCR. Bax, Bcl-2 and Fas-L were further detected by western blot analysis. RESULTS:Both fluorides similarly increased the percentage of apoptosis, while they failed to activate caspases. The Bax/Bcl-2 gene expression ratio was not altered by either fluoride treatment regardless of the type of cell. NaF varnish increased the amplification of the Fas-L gene in NIH/3T3 and HGF cells, while treatment with the TiF4 varnish resulted in a lower Bad/Bcl-2 expression ratio compared to that of the control for NIH/3T3 cells, but not for HGF cells. No effect of the fluorides was detected in the protein analysis. CONCLUSIONS:NaF and TiF4, at the studied conditions, similarly induce a low level of apoptosis, with consequent modest activation of the Bcl-2 and Fas-l-dependent signalling pathways. Generally, HGF cells are more susceptible to the fluoride effect than NIH/3T3 cells.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Aranda Salomão PM,de Oliveira FA,Dos Santos DMS,Domezi JP,Dionísio TJ,de Oliveira RC,Magalhães ACdoi
10.1016/j.archoralbio.2018.10.039subject
Has Abstractpub_date
2019-01-01 00:00:00pages
245-252eissn
0003-9969issn
1879-1506pii
S0003-9969(18)30521-1journal_volume
97pub_type
杂志文章abstract：OBJECTIVE:As shown in the quantitative suspension test adding lactoperoxidase to a thiocyanate (SCN(-)) hydrogen peroxide (H(2)O(2)) combination over the physiological saliva level has significant positive antimicrobial effects to a level of totally killing Streptococcus mutans, Streptococcus sanguinis, and Candida alb...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.04.016
更新日期：2011-12-01 00:00:00
abstract：:Destrin, an actin-binding protein, is partly phosphorylated at Ser-2 (numbering on the matured form) in the resting rat parotid gland, and beta-adrenergic or cholinergic stimulation of this gland induces its dephosphorylation. In this study, we searched for the protein kinase involved in phosphorylation of destrin. We...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00129-8
更新日期：2003-09-01 00:00:00
abstract：:Growth in body length, skeletal lengths, incisor lengths and molar root lengths were retarded in dwarf mice. The root lengths of third molars were more severely affected than those of other molars. The crown lengths at the enamel-cementum junction of upper and lower molars of most dwarf mice were significantly shorter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90135-9
更新日期：1985-01-01 00:00:00
abstract：OBJECTIVE:This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone. DESIGN:DPSCs were isolated by two digestion methods (collagenase/dispase or collagenase alone) from human third molars. Immunophenotypic featu...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.05.008
更新日期：2018-09-01 00:00:00
abstract：:Fluorescently labelled latex microspheres (0.01, 0.1 and 1.0 micron dia.) were used to establish whether oral epithelial cells could exhibit an endocytic function. Oral mucosa biopsies were incubated in organ culture at 37 degrees C for 20 h with one of the three sizes of fluorescent microspheres in the medium. Tissue...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00027-8
更新日期：1999-06-01 00:00:00
abstract：:Cementum of teeth previously analysed for fluoride was re-examined in order to determine whether or not periodontal disease had affected the thickness of the tissue. In the periodontally diseased teeth the cementum was thinner than normal in the middle region of the root. The apical cementum, however, was significantl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90132-r
更新日期：1992-08-01 00:00:00
abstract：:Although the inactivation or removal of motoneurones and muscles are known to affect development and growth of the craniofacial skeleton and masticatory muscles, the effect of these on functional activity patterns is not clear. The goal of this study was to evaluate the immediate responses of masticatory muscle activi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90135-9
更新日期：1993-04-01 00:00:00
abstract：OBJECTIVE:The hypothesis of this study was that feeding glucose instead of sucrose in the cariogenic Diet 2000 to rats weaned at age 18 days would result in greater light/dark differences in feeding activity and secretion and storage of parotid salivary enzymes. DESIGN OF STUDY:Diet 2000 and a stock commercial diet (c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.11.013
更新日期：2015-03-01 00:00:00
abstract：:Previous studies using potassium ion-selective microelectrodes have demonstrated that potassium concentrations in dental plaque fluid obtained by centrifugation are identical to whole plaque values determined immediately after collection. Such procedures were now used to examine the variations in potassium concentrati...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90030-1
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVES:The objectives are 1) to calculate the position of highly accentuated lines in dental enamel of a group of individuals from Shahr-i-Sokhta, a thriving urban centre in Bronze Age South West Asia; 2) to identify peak frequencies of physiologically stressful periods during early childhood of these individuals; ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104969
更新日期：2020-11-05 00:00:00
abstract：:Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00160-7
更新日期：2003-01-01 00:00:00
abstract：:The net surface charge of various oral streptococci were assessed by aqueous two-phase partitioning in a dextran-polyethylene glycol system. Great variability was found among individual strains within all species tested. Type 1 strains of Streptococcus sanguis serotypes which have been found to be more adherent, expos...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(81)90114-x
更新日期：1981-01-01 00:00:00
abstract：:Notch receptors are involved in cell fate decisions through the process of lateral inhibition or inductive signalling. Jagged2 belongs to the family of transmembrane proteins that serve as the ligands for Notch receptors. We have analysed the expression of the Jagged2 gene in developing mouse teeth. Jagged2 expression...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.10.006
更新日期：2005-02-01 00:00:00
abstract：:An outward flow of fluid through exposed dentine was demonstrated in anaesthetized cats. The flow was measured by observing the movement of the fat droplets of dilute milk in a glass capillary (i.d. 30 microns) with a microscope. The capillary was sealed to the dentine with a plastic cap. The resting flow rate through...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90087-o
更新日期：1992-03-01 00:00:00
abstract：:The presence of human STRO-1(+)/CD146(+) periodontal ligament (PDL) cells has been reported, but obtaining a large amount of these cells is difficult. The purpose of this study was to evaluate the percentages of STRO-1(+)/CD146(+) cells in PDL cells and determine the effects of FGF-2 on the proliferation and multiline...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.12.003
更新日期：2012-06-01 00:00:00
abstract：:Gland size, amylase activity, total proteins and DNA concentrations were measured in parotid gland during chronic heat exposure (34 degrees C). Subcellular fractionation was performed. Exposure to heat resulted in a decrease in gland size and in DNA concentration; amylase activity/100 mg tissue and amylase specific ac...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90059-6
更新日期：1983-01-01 00:00:00
abstract：:Non-invasive magnetic stimulation of the brain was earlier used to reveal the corticomotor representation of the human masseter muscle but it is unclear how motor tasks affect this map. An experimental approach incorporating transcranial magnetic stimulation of verified locations on the scalp, surface electromyography...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00051-0
更新日期：2000-09-01 00:00:00
abstract：:An appropriate model was created by the paraperiosteal injection of mustard oil (20% allyl isothiocyanate dissolved in mineral oil) into the periarticular temporomandibular tissue of anaesthetized rats. Inflammation was assessed by the plasma extravasation of Evans' blue dye bound to plasma protein. This was confirmed...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90026-5
更新日期：1992-01-01 00:00:00
abstract：:The incorporation of sodium [35S]-sulphate into the buccal and palatal minor glands of the albino rat was studied in vivo and in vitro. The biosynthesis of 35S-labelled macromolecules possessing the characteristics of sulphated glycoproteins was evident in both. Uptake of [35S]-sulphate into the palatal tissue in vivo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90156-0
更新日期：1984-01-01 00:00:00
abstract：:A mandibular kinesiograph was used to monitor the movement of the lower incisors of young dentate adults reading a standard text. An interface to a microcomputer which recorded and analysed these movements. As the tracings accumulated on the computer monitor, a characteristic pattern was produced. The dimensions of th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90068-9
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:To determine whether chewing side preference is related to handedness and lateral asymmetry of occlusal characteristics, muscular force and temporomandibular disorders (TMD) in a dentate population. DESIGN:One hundred and seventeen dentate adults participated in this cross-sectional study. Static and dynamic...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.09.006
更新日期：2009-02-01 00:00:00
abstract：OBJECTIVE:This study aimed to examine and compare the anti-caries effects of citrus lemon oil (CLO) and limonene in rats. METHODS:The minimal inhibitory concentrations of CLO and limonene were measured using the disk diffusion method. The rats were infected with Streptococcus sobrinus and assigned into four groups: (1...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104851
更新日期：2020-10-01 00:00:00
abstract：IMPORTANCE:Notch proteins are cell surface transmembrane spanning receptors which mediate critically important cellular functions through direct cell-cell contact. Interactions between Notch receptors and their ligands regulate cell fate decisions such differentiation, proliferation and apoptosis in numerous tissues. W...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.04.003
更新日期：2014-07-01 00:00:00
abstract：:Glycosaminoglycans specifically regulate the amount of calcium released from bone cultures; the mechanisms responsible for this regulation are not known. Media from glycosaminoglycan-stimulated bone organ cultures were analysed to determine (1) if specific calcium-releasing substances were selectively produced, and (2...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90081-k
更新日期：1990-01-01 00:00:00
abstract：:Twenty-eight right-handed, young adults participated in a sensory testing experiment to evaluate spatial resolution at 10 positionally matched sites on the right- and left-hand sides of the face. An adaptive psychophysical (i.e. tracking) procedure was used to estimate the threshold spatial separation for perceiving t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)00202-m
更新日期：1995-06-01 00:00:00
abstract：OBJECTIVE:We previously proposed the hamster cheek pouch model of oral cancer for BNCT studies. We herein present the biodistribution of a non-toxic boron compound, GB-10 (Na(2)(10)B10H10), in this model to assess its potential for BNCT or BNCT enhanced Fast Neutron Therapy. MATERIALS AND METHODS:We evaluated the upta...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2003.10.003
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVES:The influence of carotid artery occlusion (10, 30 and 60 min) on regulatory mechanisms implicated in the vasorelaxant responses of isolated glandular branch of rabbit facial artery to acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) was examined. DESIGN:In organ bath studies with arterial rin...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.03.006
更新日期：2010-05-01 00:00:00
abstract：:The surface zone of human deciduous and permanent enamel was examined following sectioning, polishing and etching. Three main types of surface were where prisms reached the surface, where the prisms were not discernible and those formed by a complex of both types. The aprismatic layers were measured and the percentage...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90147-9
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:A loss of functional salivary glands often occurs after radiotherapy for head and neck tumour, and causes many problems in oral health. Recently, the use of salispheres, which consist of salivary gland stem cells (SGSCs), has been suggested as therapy for these problems. However, an insufficient number of cel...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.03.003
更新日期：2015-06-01 00:00:00
abstract：OBJECTIVE:The DNA oxidation byproduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a well-known biomarker used to evaluate oxidative stress. We previously reported that the generation of reactive oxygen species (ROS) is increased in cultured gingival fibroblasts (GF) from patients with Down syndrome (DS). Thus, the aim of t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.03.017
更新日期：2013-09-01 00:00:00
