Abstract:
:Autoantibodies directed against nucleic acid and protein complexes present in cell nuclei characterize autoimmune diseases and are employed in diagnosis. The mechanisms by which these autoantigens escape immunological tolerance are largely unknown, but a number of recent observations suggest that modified self-protein generated during apoptosis my play an important part in the development of autoimmunity. To investigate the possibility that autoantibodies in patients with Sjögren's syndrome are induced by apoptosis and presented on the surface of the cell, the internal distribution of autoantigens in apoptotic human salivary gland cells was studied in vitro. Salivary gland cells were treated with tumour necrosis factor-alpha, an apoptosis inducer. At increasing times after induction, cells were homogenized and cytoplasmic, cell surface membrane and nuclear compartments were fractionated using a sucrose density-gradient system. Autoantigens alpha-fodrin, SS-A (Ro), SS-B (La), and the enzyme poly(ADP-ribose) polymerase, were detected by conventional immunofluorescence and confirmed by Western immunoblotting. At increasing times after apoptosis, nuclear proteins SS-A (Ro) and SS-B (La), but not poly(ADP-ribose) polymerase were relocated from the cell nucleus to the cell surface membrane. Fodrin, a cytoplasmic protein, was also translocated to the cell membrane after cleavage of alpha-fodrin. These results show that autoantigens fodrin, SS-A (Ro) and SS-B (La) in human salivary gland cells undergo a striking redistribution during apoptosis and relocate to the cell membrane of apoptotic cells. The appearance of autoantigens on the surface of induced cells could form the basis of a mechanism for autoantigen presentation, processing and autoantibody induction.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
McArthur C,Wang Y,Veno P,Zhang J,Fiorella Rdoi
10.1016/s0003-9969(02)00025-0subject
Has Abstractpub_date
2002-06-01 00:00:00pages
443-8issue
6eissn
0003-9969issn
1879-1506pii
S0003996902000250journal_volume
47pub_type
杂志文章abstract::The retrognathic Brachyrrhine (Br) heterozygote mouse mutant has a very localized morphological deficiency in the sphenoethmoidal region of the anterior cranial base. The purpose of this study was to test the hypothesis that a primary growth defect occurs in that region of Br mice. Primary cell cultures were derived f...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00105-4
更新日期:2002-02-01 00:00:00
abstract::The interaction between proteins and cell receptors is related to tissue homeostasis such as in salivary glands. In this respect, alterations in hormone levels caused by hyperglycaemic conditions may interfere with this interaction, intensifying the damage caused by diabetes mellitus. Hormone replacement therapy is an...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.03.014
更新日期:2011-10-01 00:00:00
abstract::The 7S nerve-growth factor (7S NGF), high molecular weight epidermal growth factor (HMW-EGF) and their subunits were isolated from mouse submandibular gland. Plasminogen activator activity was found in the 7S NGF and HMW-EGF, as well as in the gamma subunit and EGF-binding protein. Later activities were two- or three-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90094-2
更新日期:1982-01-01 00:00:00
abstract::Normal keratinocytes from epidermis and from buccal mucosa underwent dissimilar stages of differentiation in the same culture medium and responded differently to changes in the composition of the medium. Manifestations of these variations were examined in terms of the expression at the mRNA level (as measured by rever...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00046-r
更新日期:1995-09-01 00:00:00
abstract::The mineralization levels of erupted buccal enamel from 24 deciduous teeth were compared to those of 28 permanent teeth. Sections were prepared in a defined plane using a lapping machine which gave plano-parallel sections. Mineralization levels were recorded by quantitative microradiography at 25 equivalent anatomical...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90130-1
更新日期:1989-01-01 00:00:00
abstract::Natural immunity to synthetic peptides (SP) derived from the sequences of a 3800 Mr Streptococcus mutans antigen was found in human subjects. Significant serum IgG antibodies were detected both to the native streptococcal antigen and to the SP17, containing essentially residues 1-15. A series of short peptides with de...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90129-x
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVE:Detecting the distribution and intensity of NLRP3, NLRP1 and AIM2 expression in different types of periodontitis gingival tissues. MATERIALS AND METHODS:A total of 65 gingival tissues were collected from clinic and been divided into three groups: patients with chronic periodontitis (CP), patients with genera...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.03.005
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:The function of apoptosis and its regulation in odontoblasts remain unclear. In this study, we characterize the possible role of transforming growth factor (TGF)-beta 1 in the induction of apoptosis and the molecular mechanisms that mediate TGF-beta1-induced apoptosis in odontoblasts. METHODS:Annexin V/propi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.03.004
更新日期:2005-11-01 00:00:00
abstract:OBJECTIVE:To assess a panel of risk factors associated to oral candidosis (OC) onset and its chronic maintenance by means of fuzzy logic (FL) approach and statistical traditional methodology (STM); to investigate their casual relationship within a multifactorial framework. DESIGN:Case-control study. SETTING:One hospi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.11.009
更新日期:2008-04-01 00:00:00
abstract::Four-five months after the induction of diabetes, salivary tissues of male Wistar rats were preserved by glutaraldehyde fixation or rapid freezing in dry-ice cooled hexane. Fixed tissues were either processed and embedded for light and electron microscopy, or frozen and, together with unfixed tissues, sectioned and st...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90021-x
更新日期:1986-01-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have recently attracted more attention about the role in a broad range of biological processes and complex cancers. We aimed to identify differentially expressed lncRNAs that play an important role in the pathogenesis of oral squamous cell carcinoma (OSCC). Microarray data GSE25099 consi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.08.003
更新日期:2015-10-01 00:00:00
abstract::The bioconcentration of lipophilic organochlorines in ovine dentine in comparison to adipose tissue was examined. Sheep were given a single dose (0.2-1.4 mg/kg body wt) of individual polychlorobiphenyl (PCB) congeners (tetrachlorobiphenyls IUPAC Nos. 54 and 80, and hexachlorobiphenyls IUPAC Nos. 155 and 169) and organ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00079-6
更新日期:2001-12-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP). DESIGN:m-MDDCs derived from peripheral blood monocytes, cultured for 7 days ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.03.013
更新日期:2013-09-01 00:00:00
abstract::The inability of fibrocartilage, specifically the temporomandibular joint (TMJ) disc, to regenerate and remodel following injury presents a unique problem for clinicians. Tissue engineering then offers a potential regenerative therapy. In vitro testing provides a valuable screening tool for potential tissue engineered...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2017.07.013
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:As shown in the quantitative suspension test adding lactoperoxidase to a thiocyanate (SCN(-)) hydrogen peroxide (H(2)O(2)) combination over the physiological saliva level has significant positive antimicrobial effects to a level of totally killing Streptococcus mutans, Streptococcus sanguinis, and Candida alb...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.04.016
更新日期:2011-12-01 00:00:00
abstract::Soluble reduced lysozyme was extensively digested by a trypsin-like protease purified from the culture supernatant of the bacterium. The digestion peptides were separated and purified by reversed-phase high-performance liquid chromatography, and were subjected to amino acid analysis. The fragments were identified by t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90149-0
更新日期:1989-01-01 00:00:00
abstract::The recovery of fibres in the chorda tympani after repair by epineurial suture or entubulation was investigated. The combined trunk of the chorda tympani and lingual branch of the trigeminal nerve was sectioned unilaterally, repaired using either epineurial sutures or entubulation, and allowed to recover for 12 weeks....
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)00189-i
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Dental pulp repair is a common process triggered by microbial and mechanical challenges. Matricellular modulators, such as periostin, are key for extracellular matrix stability and tissue healing. In the scope of the dental pulp, periostin expression has been reported during development and act...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.02.008
更新日期:2015-05-01 00:00:00
abstract::The classical hydrodynamic theory implicated fluid movement as a transducing mechanism in the production of dental sensitivity. This theory assumes that sensitive dentine must be permeable. Various measurements of dentine permeability are discussed, including: (1) factors that influence diffusive permeation across den...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/0003-9969(94)90191-0
更新日期:1994-01-01 00:00:00
abstract:UNLABELLED:The inferior head of lateral pterygoid (IHLP) is thought to play a critical role in the generation and control of lateral jaw movements. AIM:The aim was to test the hypothesis that a change to the lateral tooth guidance (working-side occlusal alteration, OA) results in a significant change in the electromyo...
journal_title:Archives of oral biology
pub_type: 临床试验,杂志文章
doi:10.1016/j.archoralbio.2006.01.007
更新日期:2006-08-01 00:00:00
abstract::The scattering phase functions for HeNe-laser light of dentine sections 10-20 micron(s) thick were measured. The functions perpendicular to the tubules had first-order maxima at angles of 4 degrees for bovine dentine and 5 degrees for human dentine; those parallel to the tubules showed no first-order maxima. Several c...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90098-f
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms. DESIGN:AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay. In additio...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104943
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:Preservation of structural and biochemical properties of the root dentin matrix is crucial to favor healing and regenerative periodontal processes. Aim of this study was to evaluate the biochemical characteristics of collagen and chondroitin sulphate of root dentin surfaces exposed by periodontal disease afte...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2006.07.001
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:We previously proposed the hamster cheek pouch model of oral cancer for BNCT studies. We herein present the biodistribution of a non-toxic boron compound, GB-10 (Na(2)(10)B10H10), in this model to assess its potential for BNCT or BNCT enhanced Fast Neutron Therapy. MATERIALS AND METHODS:We evaluated the upta...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2003.10.003
更新日期:2004-04-01 00:00:00
abstract::The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that M...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90070-6
更新日期:1985-01-01 00:00:00
abstract::The eruption of the deciduous teeth in Down's individuals is reportedly delayed, but the extent of delay in comparison to normal children has been little studied. The eruption characteristics of the deciduous teeth in a sample of Chilean individuals with Down's syndrome were compared with those of the normal Chilean p...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(97)00012-5
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVE:To evaluate in vitro the effectiveness of different calcium-silicate (CS) cements specifically designed for dentine permeability reduction. DESIGN:Fifty dentine discs were prepared from caries free human molars extracted for surgical reasons. The treatment consisted in applying two different experimental CS ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.02.024
更新日期:2012-08-01 00:00:00
abstract::Mice lacking myostatin (GDF-8), a negative regulator of skeletal muscle growth, show a significant increase in muscle mass versus normal mice. We compared wild-type and myostatin deficient mice to assess the postnatal effect of elevated masticatory loads due to increased jaw-adductor muscle activity and greater bite f...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.05.008
更新日期:2006-01-01 00:00:00
abstract::A mandibular kinesiograph was used to monitor the movement of the lower incisors of young dentate adults reading a standard text. An interface to a microcomputer which recorded and analysed these movements. As the tracings accumulated on the computer monitor, a characteristic pattern was produced. The dimensions of th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90068-9
更新日期:1987-01-01 00:00:00
abstract::The peroxidase activity in human whole saliva is due to salivary peroxidase and, in some cases, myeloperoxidase; it is usually determined by spectrophotometric methods based on the rate of oxidation of chromogen substrates. Thiocyanate ion, a normal component of saliva, interferes with these kinetic assays by competin...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90095-6
更新日期:1986-01-01 00:00:00