Abstract:
OBJECTIVE:The present study was designed to examine how oestrogen regulates proliferation, osteoblastic differentiation, collagen synthesis and periostin gene expression in primary human periodontal ligament (hPDL) cells. DESIGN:The short interfering RNA (siRNA) technique was used to inhibit oestrogen receptor beta (ERβ) expression hPDL cells. hPDL cell were isolated and fully characterized. A colorimetric assay was applied for the determination of alkaline phosphatase (ALP). An ELISA kit was used to detect osteocalcin (OCN) levels. Collagen synthesis was determined by measuring the incorporation of L-[3H] praline. RT-PCR was performed to detection of periostin mRNA relative gene expression. RESULTS:ERβ mRNA was expressed in hPDL cells and significant inhibition of mRNA expression and ERβ mature protein of the ERβ was evident in the siRNA group. At 72h, there was a significant increase in non-transfected hPDL cell proliferation after estradiol stimulation. Addition of 17β-estradiol significantly enhanced ALP activity and production of OCN in non-transfected cells but had no effect on collagen synthesis. A clear increase in periostin mRNA expression levels was observed after incubating hPDL cells with estradiol. In hPDL-siERβ cells, the application of estradiol did not produce any evident differences in periostin mRNA expression CONCLUSIONS:ERβ may play important roles in oestrogen-induced effects on hPDL cell proliferation, osteoblastic differentiation and expression of key molecules for the functional and structural integrity of the periodontium (i.e. periostin).
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Mamalis A,Markopoulou C,Lagou A,Vrotsos Idoi
10.1016/j.archoralbio.2010.11.001subject
Has Abstractpub_date
2011-05-01 00:00:00pages
446-55issue
5eissn
0003-9969issn
1879-1506pii
S0003-9969(10)00336-5journal_volume
56pub_type
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
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doi:10.1016/j.archoralbio.2012.08.014
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90116-c
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doi:10.1016/0003-9969(88)90055-6
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.10.017
更新日期:2012-05-01 00:00:00
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90022-1
更新日期:1994-10-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.003
更新日期:2017-08-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.02.007
更新日期:2017-06-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2008.02.002
更新日期:2008-08-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.09.005
更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.12.004
更新日期:2011-07-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.09.016
更新日期:2011-03-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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doi:10.1016/j.archoralbio.2018.07.003
更新日期:2018-11-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.11.006
更新日期:2017-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90182-a
更新日期:1990-01-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.015
更新日期:2017-09-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.01.004
更新日期:2014-04-01 00:00:00