Oestrogen regulates proliferation, osteoblastic differentiation, collagen synthesis and periostin gene expression in human periodontal ligament cells through oestrogen receptor beta.


OBJECTIVE:The present study was designed to examine how oestrogen regulates proliferation, osteoblastic differentiation, collagen synthesis and periostin gene expression in primary human periodontal ligament (hPDL) cells. DESIGN:The short interfering RNA (siRNA) technique was used to inhibit oestrogen receptor beta (ERβ) expression hPDL cells. hPDL cell were isolated and fully characterized. A colorimetric assay was applied for the determination of alkaline phosphatase (ALP). An ELISA kit was used to detect osteocalcin (OCN) levels. Collagen synthesis was determined by measuring the incorporation of L-[3H] praline. RT-PCR was performed to detection of periostin mRNA relative gene expression. RESULTS:ERβ mRNA was expressed in hPDL cells and significant inhibition of mRNA expression and ERβ mature protein of the ERβ was evident in the siRNA group. At 72h, there was a significant increase in non-transfected hPDL cell proliferation after estradiol stimulation. Addition of 17β-estradiol significantly enhanced ALP activity and production of OCN in non-transfected cells but had no effect on collagen synthesis. A clear increase in periostin mRNA expression levels was observed after incubating hPDL cells with estradiol. In hPDL-siERβ cells, the application of estradiol did not produce any evident differences in periostin mRNA expression CONCLUSIONS:ERβ may play important roles in oestrogen-induced effects on hPDL cell proliferation, osteoblastic differentiation and expression of key molecules for the functional and structural integrity of the periodontium (i.e. periostin).


Arch Oral Biol


Archives of oral biology


Mamalis A,Markopoulou C,Lagou A,Vrotsos I




Has Abstract


2011-05-01 00:00:00














  • Interferon-γ +874A/T polymorphism in relation to generalized chronic periodontitis and the presence of periodontopathic bacteria.

    abstract:BACKGROUND:Interferon gamma (IFN-γ) is one of the key regulatory cytokines that has a significant effect on immune responses. It may be important in the chronic inflammatory diseases such as periodontitis in which increased IFN-γ levels were found. The aim of this study was to analyze +874A/T polymorphism in the IFN-γ ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Holla LI,Hrdlickova B,Linhartova P,Fassmann A

    更新日期:2011-02-01 00:00:00

  • The expression of periostin in dental pulp cells.

    abstract:BACKGROUND AND OBJECTIVE:Dental pulp repair is a common process triggered by microbial and mechanical challenges. Matricellular modulators, such as periostin, are key for extracellular matrix stability and tissue healing. In the scope of the dental pulp, periostin expression has been reported during development and act...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Wiesen RM,Padial-Molina M,Volk SL,McDonald N,Chiego D Jr,Botero T,Rios HF

    更新日期:2015-05-01 00:00:00

  • Prevalence of Staphylococcus aureus and methicillin resistant Staphylococcus aureus (MRSA) in the oral cavity.

    abstract:OBJECTIVE:To assess the prevalence of Staphylococcus aureus and methicillin resistant Staphylococcus aureus (MRSA) in plaque and tongue samples from systemically healthy subjects with periodontal health, gingivitis or chronic periodontitis. METHODS:After screening 720 potentially eligible subjects, 154 systemically he...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Koukos G,Sakellari D,Arsenakis M,Tsalikis L,Slini T,Konstantinidis A

    更新日期:2015-09-01 00:00:00

  • Oral health status of patients with leukemia: a systematic review with meta-analysis.

    abstract:OBJECTIVE:The aim of this study was to systematically review the literature regarding the prevalence of periodontal diseases and dental caries in patients with leukemia. METHODS:An electronic search for observational studies on oral health outcomes in patients with leukemia was performed on Medline/PUBMED, Embase, Web...

    journal_title:Archives of oral biology

    pub_type: 杂志文章,meta分析


    authors: Angst PDM,Maier J,Dos Santos Nogueira R,Manso IS,Tedesco TK

    更新日期:2020-12-01 00:00:00

  • Effects of phosphoprotein on collagen fibril formation in vitro.

    abstract::Transmission electron microscopy showed that when either dentinal phosphoprotein or calcium-treated phosphoprotein or phosvitin were introduced during type I collagen fibrillogenesis the fibrils formed were significantly wider and the cross-banding was more distinct than in the absence of phosphoprotein. The collagen ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Clarkson BH,McCurdy SP,Gaz D,Hand AR

    更新日期:1993-09-01 00:00:00

  • Infant growth, development and tooth emergence patterns: A longitudinal study from birth to 6 years of age.

    abstract:OBJECTIVE:To test the association of child's growth/development status and socioeconomic variables with dental emergence patterns. DESIGN:A sample of 359 children belonging to a birth cohort of over 5000 children born in 1993 in Pelotas (Brazil) was studied in 1998-1999. Previously trained observers collected birthwei...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Bastos JL,Peres MA,Peres KG,Barros AJ

    更新日期:2007-06-01 00:00:00

  • Studies of the chronological course of third molars eruption in a northern Chinese population.

    abstract::Dental age estimation is of great importance for individual identification in forensic medicine and many other fields of study. Among them, tooth eruption is a parameter developmental morphology that can be determined by clinical examinations or by dental X-rays. The purpose of present research is to study the chronol...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Guo YC,Yan CX,Lin XW,Zhou H,Pan F,Wei L,Tang Z,Liang F,Chen T

    更新日期:2014-09-01 00:00:00

  • Lysophosphatidic acid induces expression of genes in human oral keratinocytes involved in wound healing.

    abstract:OBJECTIVE:Epithelial cells participate in wound healing by covering wounds, but also as important mediators of wound healing processes. Topical application of the phospholipid growth factor lysophosphatidic acid (LPA) accelerates dermal wound healing and we hypothesized that LPA can play a role in human oral wound heal...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Thorlakson HH,Engen SA,Schreurs O,Schenck K,Blix IJS

    更新日期:2017-08-01 00:00:00

  • Secretory responses to autonomic stimulation of rat salivary glands following reserpine treatment.

    abstract::The response to stimulation of the parasympathetic innervation to parotid or submandibular glands of reserpinized rats was altered from that of untreated rats. Thus, acute reserpinization, like other types of sympathectomy, resulted in increase in volume of parasympathetically-evoked parotid or submandibular saliva wh...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Jirakulsomchok D,Yu JH,Schneyer CA

    更新日期:1984-01-01 00:00:00

  • Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing.

    abstract:OBJECTIVES:The objective of this study was to evaluate the effect of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) on bone healing. STUDY DESIGN:Twelve rabbits were included in this randomized, blinded, prospective study. 15-mm×10-mm-sized defects were created in the pari...

    journal_title:Archives of oral biology

    pub_type: 杂志文章,随机对照试验


    authors: Kim TH,Kim SH,Sándor GK,Kim YD

    更新日期:2014-05-01 00:00:00

  • Gender-, side- and site-dependent variations in human perioral spatial resolution.

    abstract::Twenty-eight right-handed, young adults participated in a sensory testing experiment to evaluate spatial resolution at 10 positionally matched sites on the right- and left-hand sides of the face. An adaptive psychophysical (i.e. tracking) procedure was used to estimate the threshold spatial separation for perceiving t...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Chen CC,Essick GK,Kelly DG,Young MG,Nestor JM,Masse B

    更新日期:1995-06-01 00:00:00

  • Assessment of periodontal mechano-nociceptive function in healthy Chinese individuals.

    abstract:OBJECTIVE:Few clinical techniques are available for the description of mechano-nociceptive function in human periodontal tissues. The objective of this study was to test a new technique for assessment of periodontal mechano-nociceptive function by direct application of controlled forces to the teeth and quantify site, ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Liu R,Gu X,Zhang J,Yu L,Chen W,Wang K,Svensson P

    更新日期:2016-11-01 00:00:00

  • Analysis of thermal-induced dentinal fluid flow and its implications in dental thermal pain.

    abstract:OBJECTIVES:The initiation of the pain sensation experienced following the thermal stimulation of dentine has been correlated with fluid flow in the dentinal tubules. There may be other mechanisms. METHODS:This study examines this possibility using a mathematical model to simulate the temperature and thermal stress dis...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Lin M,Liu S,Niu L,Xu F,Lu TJ

    更新日期:2011-09-01 00:00:00

  • Altered phenotype and function of dendritic cells in individuals with chronic periodontitis.

    abstract:OBJECTIVE:To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP). DESIGN:m-MDDCs derived from peripheral blood monocytes, cultured for 7 days ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Cury PR,Carmo JP,Horewicz VV,Santos JN,Barbuto JA

    更新日期:2013-09-01 00:00:00

  • IL-25 promotes Th2-type reactions and correlates with disease severity in the pathogenesis of oral lichen planus.

    abstract:OBJECTIVE:The aim of the present study was to investigate the correlation between IL-25 expression and disease severity, and the potential immunoregulatory role of IL-25 expression in oral lichen planus (OLP). MATERIALS AND METHODS:The oral mucosal tissue samples obtained from OLP patients and healthy controls (HCs) w...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Wang H,Jiang Y,Wang H,Luo Z,Wang Y,Guan X

    更新日期:2019-02-01 00:00:00

  • The rate of incisor dentine calcification and of mandibular growth in the molar region of the ovariectomized rat.

    abstract::In 14 ovariectomized and 15 control rats, killed at intervals over a 117-day period, the height of the mandible in the molar region was determined radiographically. Mandibular height was significantly related to time after operation in both groups, but their rate of mandibular growth was not significantly different. I...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Devlin H,Ferguson MW

    更新日期:1990-01-01 00:00:00

  • Oral biofilm uptake of mineral ions released from experimental toothpaste containing surface pre-reacted glass-ionomer (S-PRG) filler.

    abstract:OBJECTIVE:To clarify the fluoride/mineral kinetics in an oral biofilm following concurrent application of fluoride and other mineral ions released from experimental toothpaste containing S-PRG filler using depth-specific analysis. STUDY DESIGN:Twenty subjects wore in situ plaque-generating devices, comprised of a pair...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Kato K,Tamura K,Shimazaki Y

    更新日期:2020-09-01 00:00:00

  • Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium.

    abstract:OBJECTIVE:We aimed to differentiate dental pulp stem cells (DPSC) to odontoblast-like cells (ODPSC) and to investigate their attachment and growth on dentin in the presence of extra calcium by colorimetric assay and scanning electron microscopy (SEM). METHODS:After isolation of DPSC, they were differentiated to ODPSC....

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Özdal-Kurt F,Şen BH,Tuğlu I,Vatansever S,Türk BT,Deliloğlu-Gürhan I

    更新日期:2016-08-01 00:00:00

  • The effect of subinhibitory concentrations of gentian violet on the germ tube formation by Candida albicans and its adherence to oral epithelial cells.

    abstract:OBJECTIVE:This study investigated the effect of subinhibitory concentrations of gentian violet on the germ tube formation by Candida albicans and its adherence ability to oral epithelial cells. METHODS:Thirty strains of C. albicans isolated from denture wearers, normal healthy individuals and HIV positive patients wer...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Mafojane T,Shangase SL,Patel M

    更新日期:2017-10-01 00:00:00

  • Laser irradiation did not increase the proliferation or the differentiation of stem cells from normal and inflamed dental pulp.

    abstract:OBJECTIVE:Low-level laser therapy (LLLT) has been reported to be responsible for promoting photostimulatory and photobiomodulatory effects in vivo and in vitro, stimulating cell growth, increasing cell metabolism, improving cell regeneration and invoking an anti-inflammatory response. This study was performed in order ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Pereira LO,Longo JP,Azevedo RB

    更新日期:2012-08-01 00:00:00

  • Rho kinase mediates Porphyromonas gingivalis outer membrane vesicle-induced suppression of endothelial nitric oxide synthase through ERK1/2 and p38 MAPK.

    abstract:OBJECTIVE:To investigate the effect of Rho kinase (ROCK) on Porphyromonas gingivalis outer membrane vesicles (OMVs)-induced suppression of endothelial nitric oxide synthase (eNOS) and explore the potential mechanism. DESIGN:Firstly, we investigated the effect of OMVs on total eNOS expression and eNOS activity in Human...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Jia Y,Guo B,Yang W,Zhao Q,Jia W,Wu Y

    更新日期:2015-03-01 00:00:00

  • Radiographic measurement of the cervical spine in patients with temporomandibular dysfunction.

    abstract:AIM:To compare the craniocervical angles and distances between temporomandibular dysfunction (TMD) and free TMD subjects. CASUISTIC AND METHODS:The sample consisted of young adults, of both genders, with age ranging between 18 and 30 years. TMD diagnosis was based on the clinical criteria of the Research Diagnostic Cr...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: de Farias Neto JP,de Santana JM,de Santana-Filho VJ,Quintans-Junior LJ,de Lima Ferreira AP,Bonjardim LR

    更新日期:2010-09-01 00:00:00

  • Epithelial dimensions and capillary supply in the oral mucosa of the rat.

    abstract::Subdividing the cheek, palate and ventral tongue into seven anatomically identifiable regions enabled us to assess relationships that were previously obscured by the great heterogeneity of rat oral epithelium. Capillaries were visualized by an alkaline phosphatase azo dye method in oral mucosa of four 3-month old male...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Stablein MJ,Meyer J,Waterhouse JP

    更新日期:1982-01-01 00:00:00

  • The effect of cholera toxin and epidermal growth factor on the in-vitro growth of human oral epithelial cells.

    abstract::Human epithelial cells isolated from adult gingival and infant palatal biopsies were cultured using 3T3 feeder cells. The colony-forming efficiency was about 0.8 per cent with cholera toxin and epidermal growth factor (EGF). The cell yield of cultures from infant palates depended on the concentration of cholera toxin ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Müller-Glauser W,Preisig E

    更新日期:1983-01-01 00:00:00

  • Iron chelator differentially activates macrophage inflammatory protein-3alpha/CCL20 in immortalized and malignant human oral keratinocytes.

    abstract::Macrophage inflammatory protein-3alpha (MIP-3alpha or CCL20) is an intriguing molecule in cancer immunotherapy, but MIP-3alpha expression and signalling are not well understood in oral cancer cells. We investigated CCL20 expression and signal transduction by treating immortalized human oral keratinocyte (IHOK) and ora...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Lee SK,Lee J,Min SK,Won DH,Lee YM,Lim HD,Lee W,Pae HO,Chung HT,Jun CD,Lee SK,Kim EC

    更新日期:2008-09-01 00:00:00

  • Disaccharide analysis of chondroitin sulphate in human gingival crevicular fluid using high-performance liquid chromatography.

    abstract::Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Okazaki J,Kamada A,Matsukawa F,Sakaki T,Gonda Y

    更新日期:1995-08-01 00:00:00

  • IL-6 regulates stress-induced REX-1 expression via ATP-P2Y1 signalling in stem cells isolated from human exfoliated deciduous teeth.

    abstract:OBJECTIVE:To investigate the relationship between ATP and IL-6 in mechanical stress-induced REX-1 expression in SHEDs. METHODS:Cells were stimulated with mechanical stress (0-2.5 gcm(-2)), IL-6 (0.1-5 ng/ml), or ATP (10-100 μM) for 2h in serum-free media. IL-6 and REX-1 expression was examined by qualitative and quant...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Govitvattana N,Osathanon T,Toemthong T,Pavasant P

    更新日期:2015-01-01 00:00:00

  • From periodontal mechanoreceptors to chewing motor control: A systematic review.

    abstract:PURPOSE:This critical review summarizes the current knowledge of the structural and functional characteristics of periodontal mechanoreceptors, and understands their role in the signal pathways and functional motor control. METHOD:A systematic review of the literature was conducted. Original articles were searched thr...

    journal_title:Archives of oral biology

    pub_type: 杂志文章,评审


    authors: Piancino MG,Isola G,Cannavale R,Cutroneo G,Vermiglio G,Bracco P,Anastasi GP

    更新日期:2017-06-01 00:00:00

  • Salivary aldehyde dehydrogenase--reversible oxidation of the enzyme and its inhibition by caffeine, investigated using fluorimetric method.

    abstract:OBJECTIVE:We have applied fluorimetric method to monitor aldehyde dehydrogenase (ALDH*) activity in human saliva samples to study inactivation, reactivation and inhibition of the enzyme. DESIGN:Saliva samples were collected to buffer stock solution, containing various thiols, and assayed in the presence of the fluorog...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Wierzchowski J,Pietrzak M,Szelag M,Wroczyński P

    更新日期:2008-05-01 00:00:00

  • Detection of Epstein-Barr virus and human herpes virus type 6 in saliva from patients with lymphoproliferative diseases by the polymerase chain reaction.

    abstract::Direct detection of these viruses was made by using the PCR for amplifying viral DNA. In virtually all adults low levels of the herpesvirus can be detected. Therefore it is necessary to quantitate the amount of viral DNA, and a method to compare the plasmid containing the cloned target gene was used here. After 35 cyc...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Saito I,Nishimura S,Kudo I,Fox RI,Moro I

    更新日期:1991-01-01 00:00:00