Abstract：
:The purpose of the study was to test the hypothesis that both insoluble pure type I collagen from bovine Achilles tendon and dentine collagen in root dentine powder from human teeth required acid pretreatment for subsequent degradation by trypsin, a non-specific protease. Pure type I collagen or dentine powder was treated with lactic acid, at pH 4 or 5.5, or distilled, deionized water (pH 7) as a negative control. After incubation at 37 degrees C for 24 h, extracts of pure type I collagen solutions were analysed for soluble collagen with the hydroxyproline assay. Extracts of dentine powder solution were analysed for Ca2+, total protein, final pH, and hydroxyproline. Residual, undegraded pellets were washed and then treated with trypsin or collagenase. After 24 h of incubation, the soluble fractions from the enzyme-treated pure type I collagen and dentine powder solutions were analysed for hydroxyproline. Results showed that almost no pure type I collagen was degraded during acid pretreatment. Trypsin degraded significantly more pure type I collagen in the pH 4-treated group than in the other groups. Collagenase degraded about 70% of the pure type I collagen irrespective of acid pretreatment. While acid pretreatment at pH 4 did not degrade dentine collagen, data from Ca2+ analyses and collagen breakdown by trypsin suggested that pretreatment at pH 4 demineralized and denatured dentine collagen so that the collagen could be subsequently degraded by enzymes. After pretreatment at pH 4, about 27 and 57% of the dentine collagen was degraded by trypsin and collagenase, respectively, in contrast to minimal degradation of non-acid-treated dentine collagen by the same enzymes.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Dung SZ,Li Y,Dunipace AJ,Stookey GKdoi
10.1016/0003-9969(94)90022-1subject
Has Abstractpub_date
1994-10-01 00:00:00pages
901-5issue
10eissn
0003-9969issn
1879-1506pii
0003-9969(94)90022-1journal_volume
39pub_type
杂志文章abstract：:Two mechanisms that contribute to tooth eruption are proposed from analysis of the human jaws as elastic structures which are deformed intermittently under functional occlusal loads. One possible source of eruptive force is the transient pressure gradient established within each jaw as it is deformed. The second sourc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90093-8
更新日期：1987-01-01 00:00:00
abstract：:A variable capacitance displacement transducer, previously employed for continuously monitoring movements of the rabbit mandibular incisor, was modified for use with the ferret mandibular canine. Comparing this tooth of limited growth with the tooth of continuous growth, similarities were seen in the reactions to extr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90177-2
更新日期：1983-01-01 00:00:00
abstract：:Tongue movements in three female Macaca fascicularis, with radio-opaque markers in the tongue, teeth and hyoid, feeding on apple, banana and monkey chow, were recorded using lateral projection cineradiography (+/- 100 f.p.s.) with synchronized frontal view cinephotography (50 f.p.s.). Marker positions were digitized a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)98812-d
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVE:Investigate the long-term effect of dentin pretreatment with 0.05 or 0.1% caffeic acid phenethyl ester (CAPE) on (1) bond strength of resin composite to dentin by a three-step etch-and-rinse (Adper Scotchbond Multipurpose/ ASB) or a two-step self-etch adhesive system (Clearfil SE Bond/ CSE), (2) their fractur...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.06.012
更新日期：2018-10-01 00:00:00
abstract：:The permeability characteristics of dentine have been used in many in vitro studies to evaluate longitudinally the efficacy of various restorative and preventative procedures. However, the permeability of human dentine increases over time after tooth extraction. The aim here was to determine if there is a steady phase...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00090-5
更新日期：2002-01-01 00:00:00
abstract：:Although most cases of cleft lip and palate are free of other developmental defects, children with isolated cleft lip/palate are at enhanced risk of delayed growth and reduced final size. Three variables were assessed in the permanent dentition away from the cleft site: congenital absence by tooth type (which ranged f...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90210-2
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the factors that could participate on salivary glands hypofunction during inflammation and the participation of endocannabinoids in hyposalivation induced by the presence of inflammogens in the submandibular gland (SMG) or in the brain. DESIGN:Salivary secretion was a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.04.003
更新日期：2013-09-01 00:00:00
abstract：:Covalent intermolecular cross-links in collagen provide the dentine matrix with stability and tensile strength. The density of collagen cross-links varies depending on the site within the same tissue. This variation is probably due to factors such as the different amounts of stress and different turnover rates at the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90118-6
更新日期：1993-07-01 00:00:00
abstract：OBJECTIVE:This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone. DESIGN:DPSCs were isolated by two digestion methods (collagenase/dispase or collagenase alone) from human third molars. Immunophenotypic featu...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.05.008
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:Integrin-α5 (ITGA5) gene has been reported to be critical for the progression of several cancers. However, the effects of ITGA5 in oral squamous cell carcinoma (OSCC) remain unclear. METHODS:We firstly used bioinformatics methods to analyze the ITGA5 gene expression based on the public dataset. HO1-N-1 and ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.03.007
更新日期：2019-05-01 00:00:00
abstract：BACKGROUND:Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteoblast secretion of IL-6 plays an important role in the regulation of bone metabolism. Angiotensin II (Ang II) has been shown to regulate the expression of potent inflammatory factors, including MCP-1 and IL-6, by stimulating en...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.09.016
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to evaluate the effects of the combined use of dentin matrix protein-1 (DMP1) gene-modified bone marrow stromal cells (BMSCs) and Bio-Oss(®) for maxillary sinus floor augmentation (MSFA) implant placement in dogs. MATERIALS AND METHODS:BMSCs were derived from bone marrow of six beagl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.01.004
更新日期：2016-04-01 00:00:00
abstract：:The study examined the glucose clearance (retention) in saliva at different surfaces of these teeth in 23 subjects. The mouth was thoroughly rinsed for 15 s with 20 ml of a 0.5 M glucose solution. The concentrations of glucose absorbed by small pieces of paper, placed on the mesial, distal, labial (buccal), lingual an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90183-m
更新日期：1993-06-01 00:00:00
abstract：:The accessory innervation of the mandibular dentition was examined by use of the retrograde transportation of horseradish peroxidase or wheat-germ agglutinin-horseradish peroxidase. Nerves within the cervical plexus which contained transported horseradish peroxidase included the great auricular and transverse cervical...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90129-a
更新日期：1993-07-01 00:00:00
abstract：OBJECTIVE:Calbindin D9k (CB9k) and D28k (CB28k) are intracellular soluble calcium-binding proteins, whose expressions are considered to be regulated by vitamin D. However, the amount of CB28k expression in the kidneys of vitamin D receptor-null mice was reported to be similar to that in wild type mice, suggesting no de...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2007.09.009
更新日期：2008-02-01 00:00:00
abstract：:Internal derangement (ID) in the temporomandibular joint (TMJ) comprises a group of clinical problems with relatively high prevalence. However, the temporal changes in gene expression of condylar cartilage during continuous ID remain unclear. The aim of the current study is to investigate by microarray analysis, the d...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.11.006
更新日期：2017-02-01 00:00:00
abstract：:This study sought to determine if the diet-induced changes in submandibular salivary glands can be reversed. Two groups of male weanling Sprague-Dawley rats were fed purified diets containing 9% butter + 1% corn oil (group I, control) or 9% ethyl ester concentrate of n-3 fatty acids + 1% corn oil (group II, experiment...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90209-5
更新日期：1993-05-01 00:00:00
abstract：OBJECTIVE:The focus of the current study was to identify if a possible association between NLRP3 (rs4612666) and IL-1B (rs1143634) single-nucleotide polymorphisms (SNPs) may be implicated in the etiopathogenesis of chronic periodontitis (CP) in a Colombian population. DESIGN:One hundred and twenty-four CP subjects and...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.01.013
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:Genistein is a major isoflavone subclass of flavonoids found in soybean and a potent tyrosine kinase inhibitor. The present study aimed to assess the effect of genistein on the production of proinflammatory mediators in murine macrophages stimulated with lipopolysaccharide (LPS) isolated from Prevotella inter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.11.019
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:Amelogenesis imperfecta (AI) is a group of clinically and genetically heterogeneous inherited conditions, causing alterations in the structure of enamel and chemical composition of enamel matrix during development. The objective of this study was to compare the clinical, radiographic, histological and immunoh...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.06.016
更新日期：2015-09-01 00:00:00
abstract：:One of the most popular drinks worldwide, tea is rich in polyphenols and is beneficial to our health because it contributes to the prevention of many diseases. In the human oral cavity, there are more than 750 different species of bacteria living together within dental plaque. Some of the bacteria are pathogens that c...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2019.03.027
更新日期：2019-06-01 00:00:00
abstract：:Dentinal fluid volume and protein concentrations were measured from cavities prepared in the dentine of dog molars. Fluid was collected under spontaneous conditions, during the application of negative pressures, and during a recovery period. The data allowed the calculation of dentine permeability as hydraulic conduct...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90072-3
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVES:To analyse age- and gender-related microscopic changes in sublingual glands obtained from human cadavers. STUDY DESIGN:One hundred eighty six human sublingual glands were obtained from 93 autopsies. Approximately five individuals of each gender per decade of life were allocated to three age groups: group I ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.10.019
更新日期：2005-06-01 00:00:00
abstract：:Iron-deficiency anaemia was induced in hamsters by feeding a low-iron diet coupled with weekly bleeding. Respiration, assessed by measuring oxygen uptake, was significantly reduced (p less than 0.002) in cheek-pouch epithelia from iron-deficient animals. To relate data on oxygen consumption to cell numbers, the cell d...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90150-5
更新日期：1985-01-01 00:00:00
abstract：:We report a specimen of an insular black rat (Rattus rattus) from Illa den Colom (Menorca, Western Mediterranean) displaying a singular dental characteristic. It has no molar teeth but displays regular incisors. Its mere occurrence as a regular adult rat is puzzling and we attempted to evaluate what diet and morpholog...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.05.004
更新日期：2010-08-01 00:00:00
abstract：:These were characterized in stimulated saliva from 21 healthy volunteers using antibodies specific for human tissue-type plasminogen activator (t-PA) or urokinase. After pre-incubation with immunoglobulins, with and without specific antibodies, the fibrinolytic activity was assayed on fibrin plates containing plasmino...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90097-5
更新日期：1987-01-01 00:00:00
abstract：:Food properties influence the parameters of the masticatory process, such as jaw movement, muscle activity and chewing rate. Firm foods will require more muscle activity than softer foods. However, the influence of food hardness on chewing rate is ambiguous as both slower and higher chewing rates have been reported fo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.07.011
更新日期：2017-11-01 00:00:00
abstract：:Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00051-9
更新日期：1996-08-01 00:00:00
abstract：:Plaque from the fitting surface of upper full dentures in eight patients with healthy palatal mucosa was studied. To characterize the predominant cultivable flora, 916 isolates (100-128 from each sample) were subcultured from anaerobic roll-tubes. Streptococci constituted 0-81 per cent (median, 41 per cent) of the iso...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90101-2
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:Periodontal inflammation is driven by the coordinated action of a number of factors, including the IL-1 family. Our study aimed to examine the levels of interleukin (IL)-36β, IL-36γ and IL-33 levels in gingival crevicular fluid (GCF) from patients with different periodontal diseases. MATERIALS AND METHODS:A...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.08.021
更新日期：2015-01-01 00:00:00
