Changes in protein kinase C activity in rat calvarial bone cells cultured in a low-calcium environment.

Abstract:

:This enzyme activity was examined in bone cells cultured for 8-10 days; the calcium concentration was 1.87 +/- 0.05 (n = 10) mM in the control medium and 0.34 +/- 0.02 (n = 10) mM in the low-calcium medium. The activity was significantly lower in the low-calcium group than in the control (p less than 0.01). The cytosolic fraction decreased more than the membranous fraction. After restoration to a regular calcium environment, the protein kinase C activity recovered rapidly to near the control value. The extent of recovery was greater in the membranous than in the cytosolic fraction. These results suggest that the enzyme was inhibited in bone cells placed in a low-calcium environment, while the sensitivity in the membrane was enhanced.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Hisada Y,Matsumoto A,Yoshimura Y

doi

10.1016/0003-9969(92)90074-i

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

695-8

issue

9

eissn

0003-9969

issn

1879-1506

pii

0003-9969(92)90074-I

journal_volume

37

pub_type

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