Effect of estrogen receptor beta on the osteoblastic differentiation function of human periodontal ligament cells.

Abstract:

OBJECTIVE:To investigate the effect of estrogen receptor beta (ERbeta) on osteoblastic differentiation function of human periodontal ligament (hPDL) cells by measuring the alkaline phosphatase (ALP) activity and the production of osteocalcin (OCN) in vitro. DESIGN:We employed a short interfering RNA (siRNA) technique to inhibit ERbeta expression in hPDL cells; the cells were cultured with a saturating concentration of 17beta-estradiol (10(-7)M). ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit. RESULTS:It was shown that estradiol significantly enhanced the ALP activity and the production of OCN in hPDL cells. However, the ALP activity and the production of OCN in hPDL-siERbeta cells were not significantly changed after estradiol treatment. CONCLUSIONS:These results indicate that ERbeta may play important roles in estrogen-induced effects on osteoblastic differentiation function of PDL cells and estrogen influences the bone formation capacity of PDL cells mainly via ERbeta.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Liang L,Yu JF,Wang Y,Wang G,Ding Y

doi

10.1016/j.archoralbio.2007.12.011

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

553-7

issue

6

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(08)00005-8

journal_volume

53

pub_type

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