Abstract:
OBJECTIVE:To investigate the effect of Rho kinase (ROCK) on Porphyromonas gingivalis outer membrane vesicles (OMVs)-induced suppression of endothelial nitric oxide synthase (eNOS) and explore the potential mechanism. DESIGN:Firstly, we investigated the effect of OMVs on total eNOS expression and eNOS activity in Human Umbilical Vein Endothelial Cells (HUVECs) and if ROCK activation is involved. Furthermore, we estimated the effect of ROCK in regulating eNOS expression and the possible underlying mechanism in vitro. At last we confirmed the results by immunohisochemisty for eNOS expression in mouse aorta endothelium exposed to OMVs and inhibitors. RESULTS:We found that OMVs suppressed eNOS expression both at RNA and protein levels in a time- and dose-dependent manner. ROCK activity was observed in this process by detecting phosphorylation of myosin light chain (MLC) and myosin-associated phosphatase type 1 (MYPT-1), which lead to reduced eNOS expression. The suppression of eNOS was significantly reversed by ROCK inhibitor Y-27632. Moreover, Y-27632 pretreatment obviously inhibited the activation of ERK1/2 and p38 MAPKs induced by OMVs, whereas that of JNK was not affected. In addition, blocking ERK1/2 or p38 MAPK by PD98059 and SB203580, respectively attenuated the OMVs-induced eNOS phosphorylation. Ex vivo study shows that OMVs reduced eNOS expression in mouse aorta endothelium. Co-treatment with OMVs and inhibitors could significantly reverse the eNOS suppression. Taken together, these results demonstrate that ROCK mediated OMVs-induced eNOS suppression through ERK1/2 and p38 MAPK. CONCLUSIONS:These data suggest that ROCK may mediate OMVs-induced eNOS expression through ERK1/2 and p38 MAPK.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Jia Y,Guo B,Yang W,Zhao Q,Jia W,Wu Ydoi
10.1016/j.archoralbio.2014.12.009subject
Has Abstractpub_date
2015-03-01 00:00:00pages
488-95issue
3eissn
0003-9969issn
1879-1506pii
S0003-9969(14)00321-5journal_volume
60pub_type
杂志文章abstract::Prednisolone (2 mg/kg) was injected daily for 14 days. Collagen and protein biosynthesis were measured in isolated molar and incisor pulps by the incorporation of [14C]-proline into protein and collagen in vitro. Collagen solubility, free proline content, prolyl hydroxylase activity, collagenolytic activity and DNA an...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90153-x
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:Macrophages are involved in tissue inflammation and repair through cytokine secretion. However, the contribution of macrophages to healing and osteogenesis after tooth extraction remains unclear. Therefore, we investigated the distribution of osteoblastic cells and macrophages in the early healing process aft...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2021.105042
更新日期:2021-01-08 00:00:00
abstract::The major gamma-carboxyglutamic acid-containing protein of rabbit cortical bone isolated and purified from near-neutral (pH 7.5) EDTA-extracts by DEAE-cellulose and Sephadex G 75 column chromatography had a molecular weight of about 5600 based on integral amino-acid composition; this was confirmed by high-performance ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90149-3
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of experimentally created disordered occlusion on the mandibular condylar cartilage in terms of histological morphology and expression of MMP-3, MMP-9, TIMP-1 and aggrecan. MATERIALS AND METHODS:Eighty 8-week-old Sprague-Dawley rats were randomly divided into two experimental (Exp)...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.07.013
更新日期:2010-11-01 00:00:00
abstract::The lesion of microhypoplasia of an unerupted tooth was composed of small, irregularly distributed crystals, similar to those of immature and hypomineralized enamel. The lesion appeared to be a defect of crystal growth and maturation, probably involving a partial and momentary failure of the operating cycle of an amel...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90055-4
更新日期:1984-01-01 00:00:00
abstract::Soluble reduced lysozyme was extensively digested by a trypsin-like protease purified from the culture supernatant of the bacterium. The digestion peptides were separated and purified by reversed-phase high-performance liquid chromatography, and were subjected to amino acid analysis. The fragments were identified by t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90149-0
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of sympathetic nervous system hyperactivity on craniofacial skeletal growth in growing spontaneously hypertensive rats (SHRs). DESIGN:Craniofacial skeletal growth was compared between male SHR and Wistar-Kyoto rats (WKR) using linear measurements on lateral and transverse cephalometric...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.01.001
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:Some saliva substitutes have been shown to demineralize dentine in vitro. This effect is counteracted by the application of various fluorides. In contrast, remineralizing saliva substitutes might be supported by these treatments, depending on the dynamics during remineralization. The aim of this in vitro stud...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.01.002
更新日期:2011-08-01 00:00:00
abstract::Objective Quantitatively evaluate blood parameters of the erythrocyte lineage in patients with chronic periodontitis and healthy controls by means of a meta-analysis. Material and methods A review of literature was performed in the scientific databases for studies published before September 2016. The abstracts were ev...
journal_title:Archives of oral biology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.archoralbio.2018.10.024
更新日期:2019-01-01 00:00:00
abstract::The study aim was to determine whether prostaglandin E(2) (PGE(2)) in gingival crevicular fluid (GCF) could serve as a risk factor for periodontitis in human immunodeficiency virus-positive (HIV(+)) patients. Clinical measurements, including gingival index (GI), plaque index, bleeding index, probing depth (PD), attach...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.04.013
更新日期:2007-11-01 00:00:00
abstract:OBJECTIVE:To explore the gene mutation in an incontinentia pigmenti (IP) patient with syndromic tooth agenesis. METHODS:Long-range polymerase chain reaction (PCR) and Sanger sequencing were used to detect inhibitor of nuclear factor kappa-B kinase subunit gamma (IKBKG) mutation in the IP patient. We used the nuclear f...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.03.013
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVE:The objective of this systematic review was to assess the impact of nicotine administration on orthodontic tooth movement (OTM). METHODS:A systematic search was conducted in PubMed, Scopus, EMBASE, MEDLINE (OVID) and Web of Knowledge databases and the Preferred Reporting Items for Systematic Reviews and Meta...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.05.016
更新日期:2018-09-01 00:00:00
abstract::Basic fibroblast growth factor (bFGF) and heparan sulphate (HS) were detected immunohistochemically in mandibular condylar cartilage, and the findings compared with those on epiphyseal articular cartilage. In the condylar cartilage, both bFGF and HS were localized in chondrocytes throughout the various zones including...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00070-3
更新日期:1998-11-01 00:00:00
abstract::New protocols for regenerative endodontic treatment along with the parallel development of tissue engineering technologies are changing traditional knowledge and treatment possibilities for young patients with pulp-affected permanent immature teeth. The regeneration and completion of apical root development in patholo...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2012.11.008
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Sickle cell anaemia (SCA) is the most frequent haematological hereditary disease. Children with SCA are submitted to long-term prophylactic therapy with penicillin, but little is known about its impact on oral microflora. The aim of this study was to evaluate the oral microbial colonization of ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.05.014
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to clarify the effects of zinc chloride (ZnCl2) exposure on the induction of heme oxygenase-1 (HO-1) expression and its regulatory mechanisms in MDPC-23 mouse odontoblast-like cells. METHODS:MDPC-23 cells were incubated with ZnCl2, and the levels of HO-1 protein, phosphorylated forms...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.09.002
更新日期:2013-04-01 00:00:00
abstract::Samples of dentine from normal deciduous and permanent teeth were demineralized with EDTA and cleaved with CNBr. The resulting peptides were separated by means of electrophoresis on polyacrylamide slab gels, stained with Coomassie blue and examined by transmitted light. Final identification was performed by means of a...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90124-9
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:Solobacterium moorei is a Gram positive bacterium that has been specifically associated with halitosis. The aim of this study was to characterize volatile sulfur compound (VSC) production by S. moorei. METHODS:S. moorei was either grown or incubated in the presence of various supplements prior to determining...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.09.011
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:Increasing attention has been given to the potential protective roles of specific antioxidant nutrients found in fruits and vegetables. However, there are relatively few reports on the cancer chemopreventive effects of lycopene or tomato carotenoids in animal models. Therefore, the chemopreventive efficacy of...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.12.007
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to evaluate the possible association between two single nucleotide polymorphisms of the β-defensin 1 gene: -20 G > A and -44 C > G at 5 untranslated region and recurrent aphthous stomatitis in a cohort of Polish patients. DESIGN:One hundred and six patients suffering from recurr...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.03.016
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival ...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2017.12.022
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:Inherited congenital anomalies in tooth number, particularly hypodontia are relatively common. Although substantial progress has been made that permits a better understanding of the causes of tooth agenesis, overall knowledge of the phenotype:genotype correlations in this anomaly are still lacking. The aim in...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.11.018
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteoblast secretion of IL-6 plays an important role in the regulation of bone metabolism. Angiotensin II (Ang II) has been shown to regulate the expression of potent inflammatory factors, including MCP-1 and IL-6, by stimulating en...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.09.016
更新日期:2011-03-01 00:00:00
abstract::The mRNA for insulin-like growth factor I (IGF-I) in the submandibular gland of mature and developing rats was examined by Northern blotting and in situ hybridization with an oligonucleotide probe. In the mature adult rat, IGF-I mRNA was expressed at a higher level in the submandibular gland than in the liver, and was...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90006-8
更新日期:1993-08-01 00:00:00
abstract:OBJECTIVE:We have applied fluorimetric method to monitor aldehyde dehydrogenase (ALDH*) activity in human saliva samples to study inactivation, reactivation and inhibition of the enzyme. DESIGN:Saliva samples were collected to buffer stock solution, containing various thiols, and assayed in the presence of the fluorog...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.11.004
更新日期:2008-05-01 00:00:00
abstract::It is well accepted that reduced masticatory function induced by a diet with soft physical consistency causes alterations in the craniofacial morphology in growing animals. It is assumed that these alterations are associated with reduced proliferative activity of osteoblasts on the bone surface, indicating a significa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00057-1
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVE:To compare the effectiveness and acceptability of a newly developed pediatric sialometer as an alternative to the conventional spitting method. METHODS:A clinical study was carried out with healthy children (G1; n = 30) and oncological children (G2; n = 30). A newly developed pediatric sialometer was compare...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104847
更新日期:2020-10-01 00:00:00
abstract::Evidence was sought for urea-induced pH gradients in dental plaque microcosm biofilms cultured from the mixed salivary bacteria in a multi plaque 'artificial mouth'. Application of 500 mmol/l urea for short periods (6 min) to 5-8 mm maximum-thickness plaques induced intraplaque pH gradients of up to 0.7 pH units with ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90147-3
更新日期:1994-06-01 00:00:00
abstract::To evaluate the role played by androgens in the development and maintenance of sex differences in the proportion of muscle fibres of different phenotypes, the effects of castration in adult (>6 months old) and in young adult (2-3 months old) male rabbits was examined. Immunohistochemical methods were used to evaluate ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00030-3
更新日期:2000-08-01 00:00:00
abstract:OBJECTIVES:The influence of carotid artery occlusion (10, 30 and 60 min) on regulatory mechanisms implicated in the vasorelaxant responses of isolated glandular branch of rabbit facial artery to acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) was examined. DESIGN:In organ bath studies with arterial rin...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.03.006
更新日期:2010-05-01 00:00:00