Abstract：
OBJECTIVE:We have applied fluorimetric method to monitor aldehyde dehydrogenase (ALDH*) activity in human saliva samples to study inactivation, reactivation and inhibition of the enzyme. DESIGN:Saliva samples were collected to buffer stock solution, containing various thiols, and assayed in the presence of the fluorogenic substrate 6-dimethylamino-2-naphthaldehyde and NAD(+). Fluorescence of the produced 6-dimethylamino-2-naphthalene carboxylate was used to measure the reaction rate. RESULTS:Kinetic parameters for the highly fluorogenic substrate, 6-dimethylamino-2-naphthaldehyde were measured, with apparent K(m) of 7.9 microM at pH 7.3. The apparent K(m) for NAD(+) was 1.2 microM. The observed ALDH activity is unstable in the absence of thiols, but can be stabilized by 1mM glutathione, and inactivated enzyme can be re-activated within 10 min by treatment of 0.5 mM DTT. Two-assay procedure was applied to measure degree of inactivation of ALDH in saliva samples. It was found that degree of ALDH inactivation in fresh samples, stabilized by glutathione, is between 0% and 90%, with average value ca. 40%. Caffeine and theophylline were shown to be moderate inhibitors of salivary ALDH. CONCLUSIONS:Oxidation of the salivary ALDH in fresh saliva may be reliably measured using fluorimetric two-assay procedure. Preliminary statistics indicate that in most individuals this enzyme is partially inactive. Inhibition of the salivary ALDH by caffeine may have consequences for nutrition safety.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Wierzchowski J,Pietrzak M,Szelag M,Wroczyński Pdoi
10.1016/j.archoralbio.2007.11.004subject
Has Abstractpub_date
2008-05-01 00:00:00pages
423-8issue
5eissn
0003-9969issn
1879-1506pii
S0003-9969(07)00285-3journal_volume
53pub_type
杂志文章abstract：:In adult miniature pigs (Sus scrofa) fibres of the masseter contract differentially, helping to produce the successive movements of the mandible during the chewing cycle. In infant pigs, however, most fibres of the masseter contract simultaneously. One hypothesis to explain the ontogenetic change in contraction patter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90116-c
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:Vanillic acid, also known as 4-hydroxy-3-methoxy benzoic acid has a potent effect on bone metabolism. The purpose of the present study was to specify the effects of vanillic acid (VA) on preventing inflammation and bone destruction in experimental periodontitis as inflammatory bone disease. To evaluate the ef...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.05.010
更新日期：2019-07-01 00:00:00
abstract：:Streptococcus mutans GS5, Lactobacillus casei DSM20011 and Actinomyces viscosus T14 produce artificial caries in the roots of extracted teeth. Roots were coated with wax leaving an 8 mm2 window exposed on the buccal surfaces, and then incubated for 8 days in the presence of the test organism, the synthetic medium bein...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90031-3
更新日期：1988-01-01 00:00:00
abstract：AIM:Different enzyme-containing toothpastes are available on the market. The aim of the present in situ study was to investigate their efficacy for immobilisation of protective enzymes in the pellicle layer. METHODS:Pellicle formation took place in situ on bovine enamel slabs fixed to individual upper jaw splints carr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.03.020
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:When eating solids, stage II transport (St2Tr) propels triturated food into the pharynx for bolus formation and storage before swallowing. Although the existence of St2Tr is acknowledged, the reason for its existence remains unclear. Understanding it may facilitate development of food appropriate for individu...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.05.013
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVE:Respiratory epithelial cells are the first natural barrier against bacteria and viruses; hence, the interactions among epithelial cells, bacteria, and viruses are associated with disease occurrence and development. The effect of co-infection by P. gingivalis and influenza A virus (IAV) on respiratory epitheli...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.04.003
更新日期：2018-11-01 00:00:00
abstract：:Internal derangement (ID) in the temporomandibular joint (TMJ) comprises a group of clinical problems with relatively high prevalence. However, the temporal changes in gene expression of condylar cartilage during continuous ID remain unclear. The aim of the current study is to investigate by microarray analysis, the d...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.11.006
更新日期：2017-02-01 00:00:00
abstract：:The apparent diffusion rate, D, of lactate was significantly retarded in dental plaque fluid and a simulated plaque fluid consisting of a chemically-defined solution of salts, amino acids and albumin in phosphate buffer at pH 6.5. Metabolic utilization of lactate in live plaque residue reduced D for lactate into such ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90106-2
更新日期：1985-01-01 00:00:00
abstract：:Recent studies of the manipulation and reduction of food in the human mouth have used lateral-projection videofluorography (VFG) and so have concerned antero-posterior food movement. To determine the degree to which (a) food was chewed bilaterally as opposed to unilaterally, (b) the frequency of side changes, (c) the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00007-9
更新日期：2002-04-01 00:00:00
abstract：:Fibronectin (FN) with an immunohistochemical fluorescence technique using anti-human FN and anti-rat FN sera was localized in the basement membrane of lip, buccal mucosa, palate, tongue, around ectopic sebaceous glands and around acini and ducts of labial salivary glands though in different amounts. In the mucosal lam...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90010-3
更新日期：1982-01-01 00:00:00
abstract：:Medications commonly used in elderly people cause hyposalivation and are associated with an enhanced prevalence of dental caries. Propranolol (a beta-adrenergic antagonist) is a commonly used antihypertensive agent that is prescribed for long-term use. The purpose of this investigation was to compare the effects of th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90094-3
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:The aim of the present study was to investigate the correlation between IL-25 expression and disease severity, and the potential immunoregulatory role of IL-25 expression in oral lichen planus (OLP). MATERIALS AND METHODS:The oral mucosal tissue samples obtained from OLP patients and healthy controls (HCs) w...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.11.015
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVE:Currently there is still a debate about whether peritubular dentine (PTD) is non-collageneous or collageneous tissue. The chemical composition and structure of human PTD and intertubular dentine (ITD) was re-visited in this study. DESIGN:The dentine tubular region including ITD and PTD prepared from human th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.09.008
更新日期：2012-04-01 00:00:00
abstract：:Mandibular first-molar tooth germs were dissected from 16-day-embryo and new-born CD1 mice. By incubation in collagenase they were separated into enamel organ, papilla and follicle. Dental pulp was obtained from mandibular first molars of 3-, 7- and 10-day-old mice. Various combinations of epithelial and mesenchymal t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90022-7
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Sickle cell anaemia (SCA) is the most frequent haematological hereditary disease. Children with SCA are submitted to long-term prophylactic therapy with penicillin, but little is known about its impact on oral microflora. The aim of this study was to evaluate the oral microbial colonization of ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.05.014
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:The molecular variation of paired domain box gene 9 (PAX9) was previously investigated by our research group and a high degree of evolutionary conservation in coding and non-coding regions was observed except in exon 3. PAX9 is a transcription factor important in tooth development, and we wanted to verify its...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.10.020
更新日期：2011-04-01 00:00:00
abstract：OBJECTIVE:The present study evaluated the nociceptive response induced by dentin hypersensitivity after dental erosion in rats that were exhibited to unpredictable chronic mild stress (UCMS)-induced depressive-like behavior. DESIGN:Adult male rats were subjected to UCMS (depression [D] group) or not (no depression [ND...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.005
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVE:Dental caries is one of the most common infectious diseases in humans. Older adults retain more teeth than did earlier generations and thus are at high risk of root caries. The root surface is covered by cementum, which facilitates the spread of caries lesions into dentinal tissues. Propionibacterium acidifac...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.06.005
更新日期：2019-09-01 00:00:00
abstract：:Dental age estimation is of great importance for individual identification in forensic medicine and many other fields of study. Among them, tooth eruption is a parameter developmental morphology that can be determined by clinical examinations or by dental X-rays. The purpose of present research is to study the chronol...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.05.018
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE:We previously proposed the hamster cheek pouch model of oral cancer for BNCT studies. We herein present the biodistribution of a non-toxic boron compound, GB-10 (Na(2)(10)B10H10), in this model to assess its potential for BNCT or BNCT enhanced Fast Neutron Therapy. MATERIALS AND METHODS:We evaluated the upta...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2003.10.003
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:To assess a panel of risk factors associated to oral candidosis (OC) onset and its chronic maintenance by means of fuzzy logic (FL) approach and statistical traditional methodology (STM); to investigate their casual relationship within a multifactorial framework. DESIGN:Case-control study. SETTING:One hospi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.11.009
更新日期：2008-04-01 00:00:00
abstract：:The permeability characteristics of dentine have been used in many in vitro studies to evaluate longitudinally the efficacy of various restorative and preventative procedures. However, the permeability of human dentine increases over time after tooth extraction. The aim here was to determine if there is a steady phase...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00090-5
更新日期：2002-01-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to assess whether or not Vitamin D deficiency was associated with the GCF and gingival tissue antimicrobial peptides (AMP), namely, human beta defensin-2 (hBD-2) and cathelicidin (LL-37) level in chronic periodontitis (CP) and gingivitis patients. DESIGN:A total of 80 volunteers were...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104827
更新日期：2020-09-01 00:00:00
abstract：:The incorporation of sodium [35S]-sulphate into the buccal and palatal minor glands of the albino rat was studied in vivo and in vitro. The biosynthesis of 35S-labelled macromolecules possessing the characteristics of sulphated glycoproteins was evident in both. Uptake of [35S]-sulphate into the palatal tissue in vivo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90156-0
更新日期：1984-01-01 00:00:00
abstract：:A series of unstimulated and stimulated saliva samples from healthy volunteers was tested for EGF by radioimmunoassay. Salivary EGF, like other salivary constituents, was secreted in a diurnal pattern. On stimulation the rate of EGF secretion doubled but the concentration fell by 70% because of dilution by salivary fl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90032-6
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the spatio-temporal relationships between articulators in the anterior oral cavity, during the production of Japanese fricative and plosive articulation using our proposed method for tooth visualization in MR image sequences. DESIGN:Ten healthy adults without malocclusion participated in the stud...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.11.002
更新日期：2012-06-01 00:00:00
abstract：:The present study was designed to investigate the mechanical properties and load-relaxation of these discs in compression. Eight discs were used for the experiments. Compression was applied to specimens up to the specified strain, and a series of load-relaxation tests was conducted on each specimen from 0.25% strain t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00097-7
更新日期：1999-12-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to evaluate the effects of the combined use of dentin matrix protein-1 (DMP1) gene-modified bone marrow stromal cells (BMSCs) and Bio-Oss(®) for maxillary sinus floor augmentation (MSFA) implant placement in dogs. MATERIALS AND METHODS:BMSCs were derived from bone marrow of six beagl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.01.004
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:Calbindin D9k (CB9k) and D28k (CB28k) are intracellular soluble calcium-binding proteins, whose expressions are considered to be regulated by vitamin D. However, the amount of CB28k expression in the kidneys of vitamin D receptor-null mice was reported to be similar to that in wild type mice, suggesting no de...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2007.09.009
更新日期：2008-02-01 00:00:00
abstract：:An appropriate model was created by the paraperiosteal injection of mustard oil (20% allyl isothiocyanate dissolved in mineral oil) into the periarticular temporomandibular tissue of anaesthetized rats. Inflammation was assessed by the plasma extravasation of Evans' blue dye bound to plasma protein. This was confirmed...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90026-5
更新日期：1992-01-01 00:00:00
