Abstract:
OBJECTIVE:Several local factors can affect the wound-healing process, delaying its progression and postponing tissue homeostasis. It is known that local inflammation is related to wound healing; however, the maintenance of the inflammatory reaction can impair the proliferation and migration of oral mucosal cells. The aim of this study was to evaluate the viability and chemokine expression of epithelial cells and gingival fibroblasts exposed to long-term lipopolysaccharide (LPS) treatment. DESIGN:Epithelial cells (HaCaT, Cell Lines Service, 300493) and human gingival fibroblasts (HGFs) were seeded (1×10(5) cells/well) in 24-well plates and incubated for 24h. To simulate the responses of cells to a local chronic oral mucosal inflammation, we added LPS of Escherichia coli (10 μg/ml) to Dulbecco's modified Eagle's medium (DMEM), kept in contact with fibroblasts and epithelial cells for 24, 48, and 72h. Then the cells were assessed for viability (alamarBlue assay), number (trypan blue assay), and expression of CCL2 and CCL5 inflammatory chemokines (enzyme-linked immunosorbent assay (ELISA)). Data were statistically analyzed by nonparametric Kruskal-Wallis and Mann-Whitney tests at a significance level of 5%. RESULTS:Cell treatment with LPS caused significant decrease in viability for both cell lines. No time-dependent effect was observed for epithelial cells. However, reduction in fibroblast viability was greater at 48 and 72 h. CCL2 and CCL5 synthesis was significantly increased for both LPS-treated cells, and this expression decreased with time. CONCLUSION:The maintenance of an inflammatory cell stimulus by LPS decreases the number and viability of cultured oral mucosal cells, which may be related to delayed wound healing.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Basso FG,Soares DG,Pansani TN,Turrioni AP,Scheffel DL,de Souza Costa CA,Hebling Jdoi
10.1016/j.archoralbio.2015.04.010subject
Has Abstractpub_date
2015-08-01 00:00:00pages
1117-21issue
8eissn
0003-9969issn
1879-1506pii
S0003-9969(15)00099-0journal_volume
60pub_type
杂志文章abstract::An in-vitro system enabled variable range of continuous and intermittent compressive forces to be applied to the cartilage of 4-day-old rats under serum-free culture conditions. A continuous compressive force below 3 g did not affect the growth of the cartilage; when the force exceeded 3 g, growth ceased. Under an int...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90002-0
更新日期:1985-01-01 00:00:00
abstract::Substituting ferrets for rats and dogs as animal models for craniofacial research is favourable because of the similarity of many of the ferret's anatomical, metabolic and physiological features to those of man. Other advantages are cost-effectiveness and possibly less ethical controversy. However, information on the ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00043-2
更新日期:2002-08-01 00:00:00
abstract::The 10,000 g supernatant of wax-stimulated whole saliva was fractionated by gel filtration and its components were tested along with amino acids, small peptides and urea for their ability to stimulate this oxygen uptake, and for their effects on pH. Fractions containing the larger components, the proteins and large pe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90176-b
更新日期:1990-01-01 00:00:00
abstract::Bone morphogenetic protein-7 (BMP-7) affects differentiation of preosteoblasts enabling the resultant cells to respond optimally to acutely acting regulators. As the phosphoinositide cascade and, particularly, the calcium-mobilizing inositol 1,4,5-trisphosphate (InsP3) receptor are integral to stimulus-secretion coupl...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00122-3
更新日期:2000-02-01 00:00:00
abstract::The cartilage of the mandibular condyle of growing mice was used to monitor changes in the content of protein, DNA, hydroxyproline, hydroxylysine, uronic acid, calcium and phosphate. Protein and DNA increased in a stepwise fashion with one spurt seen during the first 2 weeks of post-natal life a second immediately aft...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90036-5
更新日期:1983-01-01 00:00:00
abstract::Previous studies have demonstrated that low-magnitude, high-frequency (LMHF) mechanical vibrations (LM<1g; HF<20-90Hz) can benefit bone formation. However, the underlying mechanism is unclear. In this study, MC3T3-E1 cells were exposed to LMHF vibrations of different magnitudes to assess the effect of vibrations on β-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.06.009
更新日期:2016-10-01 00:00:00
abstract::The peroxidase activity in human whole saliva is due to salivary peroxidase and, in some cases, myeloperoxidase; it is usually determined by spectrophotometric methods based on the rate of oxidation of chromogen substrates. Thiocyanate ion, a normal component of saliva, interferes with these kinetic assays by competin...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90095-6
更新日期:1986-01-01 00:00:00
abstract::In a previous study, it was shown that tooth germs of neonatal homozygous parathyroid hormone-related protein (PTHrP)-knockout mice are penetrated or compressed by the surrounding alveolar bone, suggesting an important role for PTHrP in the formation and activation of osteoclasts around growing tooth germs. In order t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00026-2
更新日期:2002-09-01 00:00:00
abstract::In the complex neurovascular control of the orofacial area, the only vasoconstrictor action is mediated by the sympathetic nervous system; however, its functional role is still unclear as little evidence exists of vasoconstrictor responses to physiological stimuli in both animal and human models. Aim of the present st...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2009.01.003
更新日期:2009-04-01 00:00:00
abstract::Salivary gland intralobular ducts are responsible for the modification of the electrolyte composition of the primary fluid secreted by the acini. However, the intracellular messengers that regulate this and other intralobular duct cell processes have not been fully characterized. To investigate the possibility that cA...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00028-3
更新日期:1996-07-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to assess whether or not Vitamin D deficiency was associated with the GCF and gingival tissue antimicrobial peptides (AMP), namely, human beta defensin-2 (hBD-2) and cathelicidin (LL-37) level in chronic periodontitis (CP) and gingivitis patients. DESIGN:A total of 80 volunteers were...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104827
更新日期:2020-09-01 00:00:00
abstract::Advances in biotechnology have brought gene therapy to the forefront of medical research. The concept of transferring genes to tissues for clinical applications has been discussed nearly half a century, but the ability to manipulate genetic material via recombinant DNA technology has brought this goal to reality. The ...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2014.11.018
更新日期:2015-03-01 00:00:00
abstract::Small amalgam fillings were placed in maxillary and mandibular second molar and canine teeth for cine-radiographic analysis. The rosette strain gauges were bonded bilaterally to mandibular cortical bone below the second or third molars. The monkeys were placed in a restraining chair that did not restrict normal head, ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90139-1
更新日期:1986-01-01 00:00:00
abstract::Although the formation of biofilms has been much studied, detachment of adherent cells from biofilms has been relatively neglected. Recent results have shown that adherent Streptococcus mutans cells can actively detach from epon-hydroxylapatite (EHA) rods conditioned with hog gastric mucin. The mechanisms for adherenc...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00139-9
更新日期:2000-04-01 00:00:00
abstract::The scattering phase functions for HeNe-laser light of dentine sections 10-20 micron(s) thick were measured. The functions perpendicular to the tubules had first-order maxima at angles of 4 degrees for bovine dentine and 5 degrees for human dentine; those parallel to the tubules showed no first-order maxima. Several c...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90098-f
更新日期:1991-01-01 00:00:00
abstract::Occlusal traits were determined for 47,XYY men and compared with previous determinations of occlusal morphology in other sex-chromosome anomalies and in normal women and men. The 47,XYY men, like 47,XXY men, tended to have a mesial molar occlusion and a mandibular overjet more often than did other groups, while 45,X w...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90063-e
更新日期:1992-11-01 00:00:00
abstract::In this present study we have tested the impact of porfimer sodium (Photofrin, AXCAN PHARMA Inc., Quebec, Canada) photoactivated disinfection (PD) on cells of Streptococcus intermedius in suspension. In order to provide basic data to support future clinical studies of PD in dentistry the study used exposure to Quartz-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.07.003
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN:The essential oils were characterized (GC-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104842
更新日期:2020-09-01 00:00:00
abstract::With scanning electron microscopy, globules were seen in inter-cellular spaces between ameloblasts with ruffled borders and papillary cells at early maturation stage. Numerous globules were located on longitudinal folds between parallel ridges on the sides of ameloblasts, but only a few in the papillary layer. In prev...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90121-3
更新日期:1984-01-01 00:00:00
abstract::In the majority of studies on masticatory performance, the degree of food breakdown has been determined by sieving the food with one or more sieves. The aim of the present study was to compare the results obtained with a single and a multiple sieve method, as these methods have never been compared. We analysed the che...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2003.08.007
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVES:The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival ...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2017.12.022
更新日期:2018-03-01 00:00:00
abstract::Although it is well known that somatostatin (SRIF) modulates several digestive functions, there are only a few reports about its effect on the salivary glands. Here, the action of SRIF parotid secretion was studied, in vivo and in vitro, in male Wistar rats. In vivo SRIF infusion (35 microg/kg per hr) inhibited the pa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00035-7
更新日期:1999-07-01 00:00:00
abstract::Basic fibroblast growth factor (bFGF) and heparan sulphate (HS) were detected immunohistochemically in mandibular condylar cartilage, and the findings compared with those on epiphyseal articular cartilage. In the condylar cartilage, both bFGF and HS were localized in chondrocytes throughout the various zones including...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00070-3
更新日期:1998-11-01 00:00:00
abstract::Osteoclastogenesis is the major cellular event responsible for bone loss and is triggered by inflammation. Acai-berry has proven anti-inflammatory effects. However, there is a lack of evidence for its effects on osteoclastogenesis. Thus, the aim of this study was to determine whether acai-berry extract (ABE) could inh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.03.016
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of sympathetic nervous system hyperactivity on craniofacial skeletal growth in growing spontaneously hypertensive rats (SHRs). DESIGN:Craniofacial skeletal growth was compared between male SHR and Wistar-Kyoto rats (WKR) using linear measurements on lateral and transverse cephalometric...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.01.001
更新日期:2019-03-01 00:00:00
abstract::The aim was to establish defined-species consortium plaque biofilms to investigate enamel and root caries in an artificial mouth. Strains of the putative enamel and root caries pathogens, Streptococcus mutans, Strep. sobrinus, Actinomyces naeslundii and Lactobacillus rhamnosus, were screened in batch culture for poten...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00111-9
更新日期:2000-01-01 00:00:00
abstract::Langerhans cells (LC) are dendritic cells of the immune system able to capture intraepithelial pathogens and migrate to regional lymph nodes to present them to naive T cells. Up to now immunohistological studies on human gingival LC have been carried out using antibodies against HLA-DR or CD1a molecules. A new marker ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00173-5
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVE:To explore the gene mutation in an incontinentia pigmenti (IP) patient with syndromic tooth agenesis. METHODS:Long-range polymerase chain reaction (PCR) and Sanger sequencing were used to detect inhibitor of nuclear factor kappa-B kinase subunit gamma (IKBKG) mutation in the IP patient. We used the nuclear f...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.03.013
更新日期:2019-05-01 00:00:00
abstract::Salivary proteins and glycoproteins that participate in the formation of 2-h in vivo enamel pellicle were determined utilizing polyacrylamide gel electrophoresis [sodium dodecyl sulphate (SDS)-PAGE and anionic PAGE]/Western transfer analyses, and specific radiolabelling/SDS-PAGE fluorography. The sensitivity of these ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90070-8
更新日期:1989-01-01 00:00:00
abstract::Masticatory muscle electromyograms (EMGs) were recorded while patients with bulbar myasthenia gravis chewed artificial food and compared with those of patients with ocular myasthenia gravis, patients in clinical remission who had previously suffered from bulbar myasthenia gravis and healthy individuals. Masticatory pe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(02)00014-6
更新日期:2002-05-01 00:00:00