Chemical composition and structure of peritubular and intertubular human dentine revisited.


OBJECTIVE:Currently there is still a debate about whether peritubular dentine (PTD) is non-collageneous or collageneous tissue. The chemical composition and structure of human PTD and intertubular dentine (ITD) was re-visited in this study. DESIGN:The dentine tubular region including ITD and PTD prepared from human third molars was in situ detected by means of micro-Raman spectroscopy (μRs) and atomic force microscopy (AFM). RESULTS:From the μRs study, it was found that the mineral/matrix ratios (phosphate vs. CH(2)) in PTD were ∼3 times of those in ITD. For the mineral, the differences between PTD and ITD were small, but still detectable. For the organic matrix, the intensity ratios of amide III to CH(2) in ITD were ∼1.5 times of those in PTD, indicating the structural differences. In addition, there was a higher proline/hydroxyproline content in ITD than that in PTD. However, the overall Raman peak contour in the amide regions (I & III) was similar, indicating collagen might still exist in both the ITD and PTD. An in situ AFM observation of the dentinal tubular region during EDTA etching confirmed that dentine collagen ran across from the ITD into the PTD. CONCLUSION:A phenomenon similar to that observed in the dentine-enamel junction is proposed to explain the above results. It is demonstrated that the μRs-AFM approach can be used to provide an insight into the structure of small dental tissues at the micron or sub-micron scale.


Arch Oral Biol


Archives of oral biology


Xu C,Wang Y




Has Abstract


2012-04-01 00:00:00














  • Detection and characterization of a rat parotid gland protein kinase that catalyzes phosphorylation of matured destrin at Ser-2.

    abstract::Destrin, an actin-binding protein, is partly phosphorylated at Ser-2 (numbering on the matured form) in the resting rat parotid gland, and beta-adrenergic or cholinergic stimulation of this gland induces its dephosphorylation. In this study, we searched for the protein kinase involved in phosphorylation of destrin. We...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Nakano S,Kanamori T,Suzuki M,Titani K

    更新日期:2003-09-01 00:00:00

  • Constituents of salivary supernatant responsible for stimulation of oxygen uptake by the bacteria in human salivary sediment.

    abstract::The 10,000 g supernatant of wax-stimulated whole saliva was fractionated by gel filtration and its components were tested along with amino acids, small peptides and urea for their ability to stimulate this oxygen uptake, and for their effects on pH. Fractions containing the larger components, the proteins and large pe...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Korayem M,Westbay G,Kleinberg I

    更新日期:1990-01-01 00:00:00

  • TiF4 and NaF varnishes induce low levels of apoptosis in murine and human fibroblasts through mitochondrial Bcl-2 family and death receptor signalling.

    abstract:OBJECTIVES:This study evaluated the level and mechanism of apoptosis in human gingival fibroblasts (HGF) and murine fibroblasts (NIH/3T3) treated with a titanium tetrafluoride (TiF4) varnish compared those treated with a sodium fluoride (NaF) varnish. METHODS:Cells were treated with a TiF4, NaF (both 2.45%F) or placeb...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Aranda Salomão PM,de Oliveira FA,Dos Santos DMS,Domezi JP,Dionísio TJ,de Oliveira RC,Magalhães AC

    更新日期:2019-01-01 00:00:00

  • Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle.

    abstract:AIM:Different enzyme-containing toothpastes are available on the market. The aim of the present in situ study was to investigate their efficacy for immobilisation of protective enzymes in the pellicle layer. METHODS:Pellicle formation took place in situ on bovine enamel slabs fixed to individual upper jaw splints carr...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Hannig C,Spitzmüller B,Lux HC,Altenburger M,Al-Ahmad A,Hannig M

    更新日期:2010-07-01 00:00:00

  • Histologic features and fascicular arrangement of the inferior alveolar nerve.

    abstract:OBJECTIVES:Knowledge of the various possible morphologies and courses of the inferior alveolar nerve and artery is important for successful and safe surgical procedures in the mandibular region. The purpose of this study was to verify the histologic features and fascicular arrangement of the inferior alveolar nerve and...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Lee MH,Kim HJ,Kim DK,Yu SK

    更新日期:2015-12-01 00:00:00

  • Quantification of jaw reflexes evoked by natural tooth contact in human subjects.

    abstract::Inhibitory jaw reflexes are believed to be important for protecting the teeth and temporo-mandibular structures from damage during sudden or forceful biting or mastication. Accordingly, alterations in these reflexes are sometimes implicated in aetiologies proposed for oro-facial pain syndromes, although the associatio...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Ainine S,Mason AG,Cadden SW

    更新日期:2011-09-01 00:00:00

  • Differential gene expression in the condylar cartilage of growing rabbits with temporomandibular joint anterior disk displacement-A transcriptomic study.

    abstract::Internal derangement (ID) in the temporomandibular joint (TMJ) comprises a group of clinical problems with relatively high prevalence. However, the temporal changes in gene expression of condylar cartilage during continuous ID remain unclear. The aim of the current study is to investigate by microarray analysis, the d...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Wang S,Deng L,Li Y,Wu H,Gu Z

    更新日期:2017-02-01 00:00:00

  • Inhibitory effects of citrus lemon oil and limonene on Streptococcus sobrinus - Induced dental caries in rats.

    abstract:OBJECTIVE:This study aimed to examine and compare the anti-caries effects of citrus lemon oil (CLO) and limonene in rats. METHODS:The minimal inhibitory concentrations of CLO and limonene were measured using the disk diffusion method. The rats were infected with Streptococcus sobrinus and assigned into four groups: (1...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Liu Y,Liu P,Wang L,Shi Y,Chen J,Wang H,Zhang X

    更新日期:2020-10-01 00:00:00

  • Characterization of human primary enamel organ epithelial cells in vitro.

    abstract:UNLABELLED:Tooth enamel is formed by ameloblasts, which are derived from the epithelial cells of the enamel organ. OBJECTIVE:The purpose of this study was to grow human ameloblast-like epithelial cells in culture. DESIGN:Human fetal tooth organs were isolated, and the cells were separated by digestion in collagenase/...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: DenBesten PK,Machule D,Zhang Y,Yan Q,Li W

    更新日期:2005-08-01 00:00:00

  • Immunohistochemical profile of basic fibroblast growth factor and heparan sulphate in adult rat mandibular condylar cartilage.

    abstract::Basic fibroblast growth factor (bFGF) and heparan sulphate (HS) were detected immunohistochemically in mandibular condylar cartilage, and the findings compared with those on epiphyseal articular cartilage. In the condylar cartilage, both bFGF and HS were localized in chondrocytes throughout the various zones including...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Tajima Y,Kawasaki M,Kurihara K,Ueha T,Yokose S

    更新日期:1998-11-01 00:00:00

  • Hinokitiol increases the angiogenic potential of dental pulp cells through ERK and p38MAPK activation and hypoxia-inducible factor-1α (HIF-1α) upregulation.

    abstract::Hinokitiol, a natural iron-chelating agent, is known to have diverse biological and pharmacological activities in various cell types. However, the effect of hinokitiol on dental pulp cells has not yet been reported. In this study, hinokitiol increases hypoxia-inducible factor-1α (HIF-1α) protein levels and vascular en...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Kim MK,Park HJ,Kim YD,Ryu MH,Takata T,Bae SK,Bae MK

    更新日期:2014-02-01 00:00:00

  • The effect of ageing on mucin contents in mouse submandibular glands.

    abstract::There may be deleterious effects if there is an age-dependent reduction in mucin composition or content in salivary glands. To assess whether there was an effect of age in submandibular glands from male mice aged 3 months, 10 and 20 months and 27, 29 and 30 months, mucin, protein and sialic acid were quantitated. Chan...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Denny PA,Klauser DK,Villa BJ,Hong SH,Denny PC

    更新日期:1991-01-01 00:00:00

  • The approximal bone height and intrabony defects in young adults, related to the salivary buffering capacity and counts of Streptococcus mutans and Lactobacilli.

    abstract::Using a computerized technique the bone height and prevalence of approximal periodontal intrabony defects were assessed on posterior bite-wing radiographs from 151 young adults. The results were related to the buffering capacity and counts of Streptococcus mutans and lactobacilli in whole stimulated saliva. The mean d...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Wikner S,Söder PO,Frithiof L,Wouters F

    更新日期:1990-01-01 00:00:00

  • The effect on vertical tooth mobility of extraction and immediate replacement of incisors (Macaca fascicularis).

    abstract::Intrusive displacement of 7 incisors was measured before and within 3 min of extraction. A substantial increase in intrusion with recovery from loads of 4 N followed replacement of the incisors. These changes are taken to indicate that tension is an important mechanism of support in the intact periodontal ligament. ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Picton DC

    更新日期:1991-01-01 00:00:00

  • Role of Porphyromonas gingivalis 40-kDa outer membrane protein in the aggregation of P. gingivalis vesicles and Actinomyces viscosus.

    abstract::Porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with Actinomyces viscosus cells. A 40-kDa outer membrane protein (OMP)-coding gene from P. gingivalis was cloned and the protein was found to be localized in these vesicles. The recombinant 40-kDa OMP did n...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Hiratsuka K,Abiko Y,Hayakawa M,Ito T,Sasahara H,Takiguchi H

    更新日期:1992-09-01 00:00:00

  • Profiling biomarkers in gingival crevicular fluid using multiplex bead immunoassay.

    abstract:OBJECTIVE:Biomarkers in gingival crevicular fluid (GCF) have been investigated; however, measurements were limited by the small sample volume available. The aim of this study was to determine the levels of 40 different cytokines and chemokines in GCF samples. DESIGN:Eleven patients with generalised chronic periodontit...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Shimada Y,Tabeta K,Sugita N,Yoshie H

    更新日期:2013-06-01 00:00:00

  • Oral biofilm uptake of mineral ions released from experimental toothpaste containing surface pre-reacted glass-ionomer (S-PRG) filler.

    abstract:OBJECTIVE:To clarify the fluoride/mineral kinetics in an oral biofilm following concurrent application of fluoride and other mineral ions released from experimental toothpaste containing S-PRG filler using depth-specific analysis. STUDY DESIGN:Twenty subjects wore in situ plaque-generating devices, comprised of a pair...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Kato K,Tamura K,Shimazaki Y

    更新日期:2020-09-01 00:00:00

  • Places in the dental arch that show a greater variability in tooth number, shape and position--a prevalence study.

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    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Primožič J,Farčnik F,Ovsenik M

    更新日期:2012-06-01 00:00:00

  • The influence of fluoride on the cellular morphology and synthetic activity of the rat dentine-pulp complex in vitro.

    abstract::Exposure to high fluoride concentrations in the immediate environment of the tissue is recognized to result in the post-translational modification of non-collagenous dentine extracellular matrix (ECM) components, potentially altering dentine mineralization. However, less is known about the effects of fluoride exposure...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Moseley R,Sloan AJ,Waddington RJ,Smith AJ,Hall RC,Embery G

    更新日期:2003-01-01 00:00:00

  • The effects of oxalate treatment on the smear layer of ground surfaces of human dentine.

    abstract::The layer was evaluated by scanning electron microscopy and by measurement of hydraulic conductance before and after 2-min topical treatment with potassium chloride, neutral potassium oxalate, half-neutralized oxalic acid or both neutral and acidic oxalates. The treated smear layers were then re-evaluated microscopica...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Pashley DH,Galloway SE

    更新日期:1985-01-01 00:00:00

  • Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth.

    abstract::Cell migration and replication associated with odontoblast replacement occurring soon after pulp exposure in primate teeth were studied. Class 5 cavity preparations resulting in pulp exposures were restored with a calcium hydroxide-containing capping agent and amalgam. Eighty-four and 96 h after this the animals were ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Fitzgerald M,Chiego DJ Jr,Heys DR

    更新日期:1990-01-01 00:00:00

  • Anti-inflammatory and antioxidant activity of hydroethanolic extract of Spondias mombin leaf in an oral mucositis experimental model.

    abstract:BACKGROUND:Spondias mombin has been used in folk medicine to treat inflammation in the oral cavity. This study aimed to evaluate the antioxidant and anti-inflammatory activity of S. mombin extract in an oral mucositis experimental model. METHODS:Male hamsters were orally pre-treated with hydroethanolic extract of S. m...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Sousa Gomes M,Diógenes Alves Uchoa Lins R,Zucolotto Langassner SM,Dantas da Silveira ÉJ,Gomes de Carvalho T,Diniz de Sousa Lopes ML,de Souza Araújo L,Addison Carvalho Xavier de Medeiros C,Ferreira de Carvalho Leitão R,Coelho Bernardo G

    更新日期:2020-03-01 00:00:00

  • An immunohistochemical study of the expression of heat-shock protein-25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars.

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    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Nakasone N,Yoshie H,Ohshima H

    更新日期:2006-05-01 00:00:00

  • Salivary micelles: identification of complexes containing MG2, sIgA, lactoferrin, amylase, glycosylated proline-rich protein and lysozyme.

    abstract::Micelles represent macromolecular structures in saliva and the aim of this study was to identify salivary proteins that occur in these globular particles. Micelles were isolated from whole saliva (WS) collected from three individuals and analysed in different experiments. Samples were subjected to polyacrylamide gel e...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Soares RV,Lin T,Siqueira CC,Bruno LS,Li X,Oppenheim FG,Offner G,Troxler RF

    更新日期:2004-05-01 00:00:00

  • Cytokine expression in gingival and intestinal tissues of patients with periodontitis and inflammatory bowel disease: An exploratory study.

    abstract:OBJECTIVE:To evaluate the expression of the cytokines IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IL-17A, IL-17F, sCD40L, and TNF-α in gingival tissue and intestinal mucosa of patients having both periodontitis and inflammatory bowel disease (IBD) and assess how they cluster in both tissu...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Menegat JS,Lira-Junior R,Siqueira MA,Brito F,Carvalho AT,Fischer RG,Figueredo CM

    更新日期:2016-06-01 00:00:00

  • Predominant cultivable microflora of plaque on removable dentures in patients with healthy oral mucosa.

    abstract::Plaque from the fitting surface of upper full dentures in eight patients with healthy palatal mucosa was studied. To characterize the predominant cultivable flora, 916 isolates (100-128 from each sample) were subcultured from anaerobic roll-tubes. Streptococci constituted 0-81 per cent (median, 41 per cent) of the iso...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Theilade E,Budtz-Jørgensen E,Theilade J

    更新日期:1983-01-01 00:00:00

  • Histological and histochemical observations on salivary microliths in ferret.

    abstract::The fortuitous observation of salivary microliths in ferret was pursued in the present investigation. Major salivary glands obtained post-mortem from mature ferrets of either sex were examined with the use of histology and light microscopical histochemistry for calcium, protein, amino acids, mucosubstances and hydroly...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Triantafyllou A,Fletcher D,Scott J

    更新日期:2006-03-01 00:00:00

  • Immunohistochemical expression of Tenascin in embryogenesis, tumorigenesis and inflammatory oral mucosa.

    abstract:OBJECTIVE:Tenascin is a large extracellular matrix glycoprotein that plays specific role in cell matrix interaction. This protein is mainly attracted because of its oncofetal predominance expression at epithelial-mesenchymal interaction and also been associated with inflammatory response. Thus the aim was to study the ...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Mane DR,Kale AD,Naik VV

    更新日期:2011-07-01 00:00:00

  • Degradation of insoluble bovine collagen and human dentine collagen pretreated in vitro with lactic acid, pH 4.0 and 5.5.

    abstract::The purpose of the study was to test the hypothesis that both insoluble pure type I collagen from bovine Achilles tendon and dentine collagen in root dentine powder from human teeth required acid pretreatment for subsequent degradation by trypsin, a non-specific protease. Pure type I collagen or dentine powder was tre...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Dung SZ,Li Y,Dunipace AJ,Stookey GK

    更新日期:1994-10-01 00:00:00

  • Investigation of the interleukin-1 gene cluster polymorphisms in Jordanian patients with chronic and aggressive periodontitis.

    abstract:OBJECTIVE:Interleukin-1 (IL-1) is a proinflammatory cytokine that is highly elevated in response to bacterial biofilms and is a potential risk factor for periodontal diseases. IL-1 gene polymorphisms have been associated with the IL-1 level. The aim of this study was to investigate if IL-1 gene cluster polymorphisms ar...

    journal_title:Archives of oral biology

    pub_type: 杂志文章


    authors: Karasneh JA,Ababneh KT,Taha AH,Al-Abbadi MS,Ollier WE

    更新日期:2011-03-01 00:00:00