Abstract：
OBJECTIVE:Primary pulp cell cultures are frequently used to study cellular responses, odontogenic potential and stem cell responses. Their isolation and expansion via a range of technical approaches are widely reported. The purpose of this study was to investigate the influence of isolation approach and extended expansion on cell phenotype and behaviour. DESIGN:To determine viable cell isolation, enzymatic dissociation was performed on rodent incisor pulps using collagenase, trypsin, hyaluronidase and ficin. Extended expansion culture of released cells was performed in DMEM and alpha-MEM media. Cultures were subsequently analysed for gene expression, cell proliferation, cell morphology and differentiation capacity up to passage 20. RESULTS:Data indicated that incubation of extirpated and mechanically minced rodent pulpal tissue with 0.25% Trypsin:EDTA and subsequent culture in alpha-MEM medium provided optimal conditions for maximal cell growth and expansion. Under these conditions, extended culture decreased cellular proliferative capacity up to passage 7, whilst higher passages demonstrated recovered growth rates. In general gene expression analysis of osteogenic and dentinogenic associated markers decreased with increasing passage number. Notably expression of TGFbetas-1, -2 and -3 increased up to passage 10 as did the stem cell and pericyte/myofibroblast markers, CD74, Neuroserpin and alpha-SMA. Analysis of molecular phenotypes indicated little difference in lineage differentiation capacity between earlier and later passages. CONCLUSIONS:The present study characterizes conditions for primary pulp cell isolation and expansion and indicates that both earlier and later passages maintain differentiation capacity. Continued passage however may result in selection for cells with a pericyte/myofibroblast phenotype.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Patel M,Smith AJ,Sloan AJ,Smith G,Cooper PRdoi
10.1016/j.archoralbio.2009.06.008subject
Has Abstractpub_date
2009-10-01 00:00:00pages
898-908issue
10eissn
0003-9969issn
1879-1506pii
S0003-9969(09)00172-1journal_volume
54pub_type
杂志文章abstract：:Internal derangement (ID) in the temporomandibular joint (TMJ) comprises a group of clinical problems with relatively high prevalence. However, the temporal changes in gene expression of condylar cartilage during continuous ID remain unclear. The aim of the current study is to investigate by microarray analysis, the d...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.11.006
更新日期：2017-02-01 00:00:00
abstract：:Gland size, amylase activity, total proteins and DNA concentrations were measured in parotid gland during chronic heat exposure (34 degrees C). Subcellular fractionation was performed. Exposure to heat resulted in a decrease in gland size and in DNA concentration; amylase activity/100 mg tissue and amylase specific ac...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90059-6
更新日期：1983-01-01 00:00:00
abstract：:This enzyme activity was examined in bone cells cultured for 8-10 days; the calcium concentration was 1.87 +/- 0.05 (n = 10) mM in the control medium and 0.34 +/- 0.02 (n = 10) mM in the low-calcium medium. The activity was significantly lower in the low-calcium group than in the control (p less than 0.01). The cytoso...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90074-i
更新日期：1992-09-01 00:00:00
abstract：OBJECTIVE:Amelogenesis imperfecta (AI) is a group of clinically and genetically heterogeneous inherited conditions, causing alterations in the structure of enamel and chemical composition of enamel matrix during development. The objective of this study was to compare the clinical, radiographic, histological and immunoh...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.06.016
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE:Previous evaluations of Psidium cattleianum leaf extract were not done in conditions similar to the oral environment. The aim of this study was to evaluate the effect of P. cattleianum leaf extract on enamel demineralisation, extracellular polysaccharide formation, and the microbial composition of dental biof...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.02.009
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVE:Low dose propranolol has previously been demonstrated to suppress bone remodelling. Therefore, its effect on orthodontic movement was tested. DESIGN:Rats were assigned as follows (n=5): animals with no orthodontic appliance (G1); the remaining groups were fitted with a Ni-Ti closed-coil spring ligated to the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.06.006
更新日期：2014-10-01 00:00:00
abstract：:Fibronectin (FN) with an immunohistochemical fluorescence technique using anti-human FN and anti-rat FN sera was localized in the basement membrane of lip, buccal mucosa, palate, tongue, around ectopic sebaceous glands and around acini and ducts of labial salivary glands though in different amounts. In the mucosal lam...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90010-3
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:The objective of this systematic review was to assess the impact of nicotine administration on orthodontic tooth movement (OTM). METHODS:A systematic search was conducted in PubMed, Scopus, EMBASE, MEDLINE (OVID) and Web of Knowledge databases and the Preferred Reporting Items for Systematic Reviews and Meta...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.05.016
更新日期：2018-09-01 00:00:00
abstract：:Ruthenium hexammine trichloride (RHT) used as a probe to visualize anion groups in the predentine and dentine of rat incisors, showed a complex distribution pattern including: intercellular proteoglycans (Pg), detected in the predentine as granules 10-15 nm in diameter and as filaments. Non-aggregating Pg was observed...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90051-8
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to assess the frequency of variations in tooth number, shape and position in the dental arch to determine if there are parts of the dental arch that show a higher prevalence of tooth variability. DESIGN:198 randomly selected Caucasian subjects (93 females, 105 males) aged between 10...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.11.009
更新日期：2012-06-01 00:00:00
abstract：:Mesiodistal dimensions of teeth in 59 relatives of 26 patients with oligodontia were evaluated and compared with the size of teeth of a control group (n = 66). It was found that the teeth of relatives do show reductions in size even in relatives with a full permanent dentition. In male relatives with a full permanent ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00143-3
更新日期：1996-05-01 00:00:00
abstract：:To establish a selected salivary-gland cell culture and determine the effect of neuropeptides, monolayers were cultured using 3T3 cells as a feeder layer. To confirm the origin of these cultured cells, amylase production was examined by electron microscopy and periodic acid-Schiff staining, together with immunocytoche...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00132-8
更新日期：1996-03-01 00:00:00
abstract：:Electrical activity recorded during voluntary clenching by platinum hook intra-cutaneous electrodes was significantly higher (p less than 0.01) when the electrodes were placed parallel to the muscle fibres than across the fibres. The correlation between the parallel and transverse EMG recordings was highly significant...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90110-5
更新日期：1987-01-01 00:00:00
abstract：:With scanning electron microscopy, globules were seen in inter-cellular spaces between ameloblasts with ruffled borders and papillary cells at early maturation stage. Numerous globules were located on longitudinal folds between parallel ridges on the sides of ameloblasts, but only a few in the papillary layer. In prev...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90121-3
更新日期：1984-01-01 00:00:00
abstract：:Notch receptors are involved in cell fate decisions through the process of lateral inhibition or inductive signalling. Jagged2 belongs to the family of transmembrane proteins that serve as the ligands for Notch receptors. We have analysed the expression of the Jagged2 gene in developing mouse teeth. Jagged2 expression...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.10.006
更新日期：2005-02-01 00:00:00
abstract：:The localization of cathepsin L and rat salivary cystatin-3 (RSC-3) in rat osteoclasts (rat femoral and alveolar bones) treated with or without E-64 (control) was examined immunocytochemically. In osteoclasts pretreated with E-64, immunoreactivity for cathepsin L was very weak extracellularly compared to that in the c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00003-4
更新日期：1997-04-01 00:00:00
abstract：:Recent reports characterizing the physiological properties of normal human labial acini are reviewed with particular emphasis on mechanisms related to fluid secretion. In contrast to the salivary glands of several experimental animals, human labial acinar cells do not appear to have a1-adrenergic receptors, substance ...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/s0003-9969(99)00044-8
更新日期：1999-05-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to compare tooth components (enamel and dentin) in Familial Dysautonomia (FD) and Down syndrome (DS) in order to assess the extent to which each was affected. DESIGN:The design was cross-sectional. The sample consisted of 20 FD patients and 45 DS patients. The control group comp...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.02.008
更新日期：2004-08-01 00:00:00
abstract：:Cells isolated by enzymatic digestion of gland fragments were incubated in solutions with or without Ca2+ or Na+ and exposed to monensin (10 microM) or acetylcholine (1 microM). Effects on accumulation of 22Na and on cell Ca2+ (measured with fura-2) were compared. In Ca2(+)-containing medium, accumulation of 22Na was ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90182-a
更新日期：1990-01-01 00:00:00
abstract：:The response to stimulation of the parasympathetic innervation to parotid or submandibular glands of reserpinized rats was altered from that of untreated rats. Thus, acute reserpinization, like other types of sympathectomy, resulted in increase in volume of parasympathetically-evoked parotid or submandibular saliva wh...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90040-2
更新日期：1984-01-01 00:00:00
abstract：:Non-invasive imaging techniques such as computerized tomography, magnetic resonance imaging (MRI), and ultrasonography enable measurements of the cross-section and thickness of human jaw muscles in vivo, providing an indication of the maximal force a muscle can exert. In 15 adult Caucasian men the thickness of the mas...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90061-2
更新日期：1994-12-01 00:00:00
abstract：:The influence of indigenous Streptococcus mutans on naturally-occurring levels of IgA antibodies was studied in 42 lactating females. Breast milk, parotid and whole-saliva samples were collected and analysed by the ELISA method for IgA antibodies, reacting with antigens from Strep. mutans. All salivas and breast milk ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90149-x
更新日期：1987-01-01 00:00:00
abstract：:Thirty-nine different microorganisms commonly found in supragingival plaque and salivary sediment were screened for their ability to raise the pH by producing base from arginine, lysylarginine and urea. Only Actinomyces naeslundii and Staphylococcus epidermidis showed significant pH-rise activity with all three compou...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90045-9
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:The existence of extra-pineal melatonin has been observed in various tissues. No prior studies of melatonin in human oral mucosal tissue under the condition of chronic inflammation have been reported. The aim of this study was to investigate the presence of melatonin in oral mucosal tissue of patients with or...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.007
更新日期：2017-06-01 00:00:00
abstract：:Aim of the in situ-study was to evaluate fluoride retention in sound and demineralised enamel after application of a novel water-based fluoride (0.12% F) varnish Mirafluorid (Hager and Werken, Germany) compared to the resin-based varnish (2.26% F) Duraphat (Colgate, USA). Each five enamel specimens were prepared from ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.09.003
更新日期：2005-03-01 00:00:00
abstract：:Destrin, an actin-binding protein, is partly phosphorylated at Ser-2 (numbering on the matured form) in the resting rat parotid gland, and beta-adrenergic or cholinergic stimulation of this gland induces its dephosphorylation. In this study, we searched for the protein kinase involved in phosphorylation of destrin. We...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00129-8
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:Interleukin (IL)-12 is an important cytokine for innate and adaptive immunity. We previously reported that IL-12 inhibits tumour necrosis factor (TNF)-α-mediated osteoclast formation by inducing apoptosis. We also reported that TNF-α plays an important role in mechanical loading-induced osteoclast formation a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.07.006
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVES:Radiographic dental age estimation methods are viable in the living and deceased. One such method [Kvaal et al. Forensic Sci Int 1995;74:175-85] quantified secondary dentinal deposition indirectly through measurements of tooth and pulp lengths and widths. The method is untested on non-European populations an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.08.020
更新日期：2012-03-01 00:00:00
abstract：:The aim was to examine the nature of enamel dissolution in aqueous suspensions with a high solid/solution ratio and in a CO2-rich atmosphere. Before experimentation, a water-saturated mixture of 95% N2-5% CO2 was passed through the acid solutions for 24 hr. Samples of 2 g of powdered enamel were suspended in 7 ml of e...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00043-5
更新日期：1997-09-01 00:00:00
abstract：:Masticatory muscle electromyograms (EMGs) were recorded while patients with bulbar myasthenia gravis chewed artificial food and compared with those of patients with ocular myasthenia gravis, patients in clinical remission who had previously suffered from bulbar myasthenia gravis and healthy individuals. Masticatory pe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00014-6
更新日期：2002-05-01 00:00:00
