Visualization of proteoglycans and membrane-associated components in rat incisor predentine and dentine using ruthenium hexammine trichloride.

Abstract:

:Ruthenium hexammine trichloride (RHT) used as a probe to visualize anion groups in the predentine and dentine of rat incisors, showed a complex distribution pattern including: intercellular proteoglycans (Pg), detected in the predentine as granules 10-15 nm in diameter and as filaments. Non-aggregating Pg was observed in the spaces between collagen fibres as an amorphous group substance. The dentine included smaller RHT-positive granules, observed after thin-section demineralization; pericellular aggregates, 30-50 nm in diameter, which were absent at the onset of the cell coat along the plasma-membrane of the odontoblast process and of the membrane itself. All these RHT-positive components might be Pg and/or sialoglyconjugates and glyco- or phospholipids present on the plasma membrane.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Goldberg M,Septier D

doi

10.1016/0003-9969(86)90051-8

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

205-12

issue

4

eissn

0003-9969

issn

1879-1506

journal_volume

31

pub_type

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