Abstract:
OBJECTIVE:Enterococcus faecalis is a Gram-positive, facultative anaerobic bacterium that is associated with failed endodontic cases and nosocomial infections. E. faecalis can form biofilms, penetrate dentinal tubules and survive in root canals with scarce nutritional supplies. These properties can make E. faecalis resistant to conventional endodontic disinfection therapy. Furthermore, treatment may be complicated by the fact that many E. faecalis strains are resistant to antibiotics. A potential alternative to antibiotic therapy is phage therapy. ϕEf11 is a temperate phage that infects strains of E. faecalis. It was previously sequenced and genetically engineered to modify its properties in order to render it useful as a therapeutic agent in phage therapy. In the current study, we have further genetically modified the phage to create phage ϕEf11/ϕFL1C(Δ36)PnisA. The aim of this study was to evaluate the efficacy of bacteriophage ϕEf11/ϕFL1C(Δ36)PnisA, to disrupt biofilms of two Enterococcus faecalis strains: JH2-2 (vancomycin-sensitive) and V583 (vancomycin-resistant). METHODS:24h static biofilms of E. faecalis strains JH2-2(pMSP3535 nisR/K) and V583 (pMSP3535nisR/K), formed on cover slips, were inoculated with bacteriophage ϕEf11/ϕFL1C(Δ36)PnisA. After 24 and 48h incubation, the bacterial biomass was imaged by confocal microscopy and viable cells were quantified by colony forming unit measurement. RESULTS:The results showed a 10-100-fold decrease in viable cells (CFU/biofilm) after phage treatment, which was consistent with comparisons of treated and untreated biofilm images visualized as max projections of the Z-series. CONCLUSION:The biomass of both vancomycin-sensitive and vancomycin-resistant E. faecalis biofilms is markedly reduced following infection by bacteriophage ϕEf11/ϕFL1C(Δ36)PnisA.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Tinoco JM,Buttaro B,Zhang H,Liss N,Sassone L,Stevens Rdoi
10.1016/j.archoralbio.2016.07.001subject
Has Abstractpub_date
2016-11-01 00:00:00pages
80-86eissn
0003-9969issn
1879-1506pii
S0003-9969(16)30169-8journal_volume
71pub_type
杂志文章abstract::The fortuitous observation of salivary microliths in ferret was pursued in the present investigation. Major salivary glands obtained post-mortem from mature ferrets of either sex were examined with the use of histology and light microscopical histochemistry for calcium, protein, amino acids, mucosubstances and hydroly...
journal_title:Archives of oral biology
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doi:10.1016/j.archoralbio.2005.07.009
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.01.007
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abstract:OBJECTIVE:The DNA oxidation byproduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a well-known biomarker used to evaluate oxidative stress. We previously reported that the generation of reactive oxygen species (ROS) is increased in cultured gingival fibroblasts (GF) from patients with Down syndrome (DS). Thus, the aim of t...
journal_title:Archives of oral biology
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.04.005
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
pub_type: 杂志文章
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
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journal_title:Archives of oral biology
pub_type: 杂志文章
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00030-3
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journal_title:Archives of oral biology
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doi:10.1016/0003-9969(96)00009-x
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journal_title:Archives of oral biology
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doi:10.1016/j.archoralbio.2004.10.013
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.11.001
更新日期:2011-05-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90112-x
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doi:10.1016/0003-9969(92)90078-m
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pub_type: 杂志文章,评审
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journal_title:Archives of oral biology
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更新日期:2018-09-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90079-2
更新日期:1993-09-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90072-3
更新日期:1991-01-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90128-5
更新日期:1982-01-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90132-r
更新日期:1992-08-01 00:00:00
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journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.11.006
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