Abstract:
OBJECTIVE:The study aimed at evaluating in vitro the effect of caffeine on expression of cystatin SN, a potential marker of sensitivity to bitterness in humans. METHODS:Differentiation of human submandibular gland (HSG) cells was induced by culturing cells on Matrigel. Caffeine cytotoxicity was assessed over 3 days by the Resazurin test. Finally, effects of 5, 50 and 100μM caffeine exposure on cystatin SN expression were explored over 3 days by ELISA. RESULTS:At concentrations relevant to human adult plasma levels (5, 50 and 100μM), caffeine did not affect cell viability whether cells were differentiated or not. Cystatin SN levels were overall higher in differentiated cells and increased with time in both conditions. There was a significant (p<0.001) effect of caffeine on cystatin SN expression specifically in differentiated cells. CONCLUSIONS:The HSG cell line proved to be a relevant tool to study in vitro the effect of caffeine at concentrations consistent with dietary intake in human subjects. The results suggest that salivary cystatin SN abundance may depend on caffeine intake, with possible consequences on taste sensitivity.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Dsamou M,Morzel M,Le Corre L,Séverin I,Chagnon MCdoi
10.1016/j.archoralbio.2013.06.005subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1511-6issue
10eissn
0003-9969issn
1879-1506pii
S0003-9969(13)00200-8journal_volume
58pub_type
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journal_title:Archives of oral biology
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