Caffeine increases the expression of cystatin SN in human submandibular acinar-like HSG cells.

Abstract:

OBJECTIVE:The study aimed at evaluating in vitro the effect of caffeine on expression of cystatin SN, a potential marker of sensitivity to bitterness in humans. METHODS:Differentiation of human submandibular gland (HSG) cells was induced by culturing cells on Matrigel. Caffeine cytotoxicity was assessed over 3 days by the Resazurin test. Finally, effects of 5, 50 and 100μM caffeine exposure on cystatin SN expression were explored over 3 days by ELISA. RESULTS:At concentrations relevant to human adult plasma levels (5, 50 and 100μM), caffeine did not affect cell viability whether cells were differentiated or not. Cystatin SN levels were overall higher in differentiated cells and increased with time in both conditions. There was a significant (p<0.001) effect of caffeine on cystatin SN expression specifically in differentiated cells. CONCLUSIONS:The HSG cell line proved to be a relevant tool to study in vitro the effect of caffeine at concentrations consistent with dietary intake in human subjects. The results suggest that salivary cystatin SN abundance may depend on caffeine intake, with possible consequences on taste sensitivity.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Dsamou M,Morzel M,Le Corre L,Séverin I,Chagnon MC

doi

10.1016/j.archoralbio.2013.06.005

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

1511-6

issue

10

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(13)00200-8

journal_volume

58

pub_type

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