Abstract:
:This review summarizes the strategy of cellular immortalization based on the principle of targeted oncogenesis in transgenic mice, used to establish models of transimmortalized renal proximal tubule cells, referred to as PKSV-PCT and PKSV-PR-cells, and collecting duct principal cells, referred to as mpkCCD(cl4) cells. These cell lines have maintained for long-term passages the main biochemical and functional properties of the parental cells from which they were derived. Proximal tubule PKSV-PCT and PKSV-PR cells have been proved to be suitable cell systems for toxicological and pharmacological studies. They also permitted the establishment of a model of multidrug-resistant (MDR) renal epithelial tubule cells, PKSV-PR(col50), which have served for the study of both MDR-dependent extrusion of chemotherapeutic drugs and inappropriate accumulation of weak base anthracyclines in intracellular acidic organelles. The novel collecting duct cell line mpkCCD(cl4), which has maintained the characteristics of tight epithelial cells, in particular Na(+) absorption stimulated by aldosterone, has been extensively used for pharmacological studies related to the regulation of ion transport. These cells have permitted the identification of several aldosterone-induced proteins playing a key role in the regulation of Na(+) absorption mediated by the epithelial Na(+) channel ENaC. Recent studies have also provided evidence that these cell lines represent valuable cell systems for the study of host-pathogen interactions and the analysis of the role of renal tubule epithelial cells in the induction of inflammatory response caused by uropathogens that may lead to severe renal damage.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Chassin C,Bens M,Vandewalle Adoi
10.1007/s10565-006-0169-ysubject
Has Abstractpub_date
2007-07-01 00:00:00pages
257-66issue
4eissn
0742-2091issn
1573-6822journal_volume
23pub_type
杂志文章,评审abstract::Hepatocytes have the ability to go through specialized cell cycles, which, during normal developmental liver growth, result in the formation of binuclear and polyploid cells. In the adult rat liver, the majority of the hepatocytes (about 70%) are tetraploid, 15-20% are octoploid, and only 10-15% are diploid (about 50%...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007487425047
更新日期:1997-07-01 00:00:00
abstract::The effect of noncytotoxic doses of argemone oil (AO) and butter yellow (BY), the common adulterants in edible oil, on free radical generation and signaling pathway for cell proliferation in primary cells of gall bladder (GB) was undertaken. AO and BY showed no cytotoxicity at 0.1 μl/ml and 0.1 μg/ml concentration, re...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-011-9207-5
更新日期:2012-06-01 00:00:00
abstract::We compared the effects of four quaternary benzo[c]phenanthridine alkaloids--chelerythrine, chelilutine, sanguinarine, and sanguilutine--and two quaternary protoberberine alkaloids-berberine and coptisine--on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomy...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1010907231602
更新日期:2001-01-01 00:00:00
abstract::During the last decade, the unbound glutathione content of cultured adherent cells has become a very important biological marker for many pharmacological and toxicological in vitro studies with regard to the protective role of the tripeptide in its reduced form (GSH). However, the literature does not provide extensive...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00767495
更新日期:1995-04-01 00:00:00
abstract::Many of the differentiated functions of hepatocytes are lost in culture, yet addition of certain medium supplements can aid in the retention of differentiated character. Therefore, the effect of time in monolayer culture on rat hepatocyte glutathione (GSH) synthesis and sensitivity to the GSH detoxicated xenobiotic et...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117851
更新日期:1986-12-01 00:00:00
abstract::S-(chloroethyl)-cysteine (CEC) and S-(1,2-dichlorovinyl)-cysteine (DCVC) have been proposed as intermediates in the metabolic transformation of the carcinogens 1,2-dichloroethane and 1,1,2-trichloroethylene. We have tested the ability of CEC and DCVC to induce DNA repair and genotoxic effects at the chromosomal level ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117768
更新日期:1988-12-01 00:00:00
abstract::The effectiveness of some chelating agents to mobilize cadmium from Chinese hamster ovary cells after chronic exposure (20 hr), as well as from cytosolic metallothionein, was studied. In the first protocol, the most effective substance was 2,3-dimercaptopropanol, followed by 2,3-dimercaptopropane-1-sulfonate and 2,3-d...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00141067
更新日期:1989-01-01 00:00:00
abstract::The mutation rates of tumor suppressor protein p53 gene (TP53) are high in lung adenocarcinoma and promote the development of acquired drug resistance. The present study evaluated the p53-dependent role in lung cancer cell sensitivity to PI3K-specific inhibitors, PI3K-associated inhibitors, PI3K-non-related inhibitors...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-020-09523-7
更新日期:2020-10-01 00:00:00
abstract::Presently, a large effort is being made worldwide to increase the sustainability of industrial development, while preserving not only the quality of the environment but also that of animal and human life. In this work, sea urchin early developmental stages were used as a model to test the effects of the organophosphat...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9061-2
更新日期:2008-12-01 00:00:00
abstract::The activity of chemical-induced unscheduled DNA synthesis was evaluated in hepatocyte primary cultures from Fischer 344 and Sprague-Dawley rats over a period of two years. In this two-year study hepatocytes from both sexes and strains were prepared from animals 2, 8, 14, 20 and 25 months of age and UDS was measured b...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00058451
更新日期:1987-06-01 00:00:00
abstract::In previous studies cadmium chloride (CdCl2) nonlethally inhibited Y-1 adrenal mouse adrenal tumour cell 20-dihydroxyprogesterone (20DHP) secretion, affecting unstimulated and stimulated steroidogenic pathway sites differently. We studied CdCl2 effects on unstimulated steroidogenesis using Y-1 cells incubated 0.5 h in...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00757184
更新日期:1994-02-01 00:00:00
abstract::Aldehyde dehydrogenase was measured in primary cultures of hepatocytes obtained with a two-step collagenase perfusion either from human hepatic tissue or from livers of Fisher rats. Basal enzyme activity declines gradually as a function of time in culture, but remains at all times higher when measured with propionalde...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00122694
更新日期:1986-06-01 00:00:00
abstract::Although the interactions of sulfur mustard (HD) with nucleic acids and proteins have been well studied, the toxic interactions with the membrane matrix and specially the phospholipid bilayer have so far been poorly investigated. We have used several NMR techniques to study these interactions: 1H NMR to observe the lo...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1020815723009
更新日期:2002-01-01 00:00:00
abstract::The article Is the clinical lipidomics a potential goldmine?, written by Linlin Zhang, Xianlin Han and Xiangdong Wang, was originally published electronically on the publisher's internet portal (currently SpringerLink) on 21 July 2018 with open access. With the author(s)' decision to step. ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10565-019-09490-8
更新日期:2020-06-01 00:00:00
abstract::A method for the primary culture of rat liver cells on collagen-coated dextran microcarriers is described. Ethoxycoumarin deethylase (EOD) activity 24 hr after inoculation was comparable for liver cells cultured on microcarriers and on collagen-coated dishes. Cells were cultured on microcarriers for up to 48 hr and re...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00124073
更新日期:1991-10-01 00:00:00
abstract::Magnesium, an essential metal that is important in the normal functioning of DNA, has been shown to interact with some of the toxic heavy metals in respect to biochemical and molecular mechanisms and in altering the tumorigenic process. This study examined the influence of magnesium in combination with nickel and cadm...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00756493
更新日期:1994-04-01 00:00:00
abstract::In pharmaceutical research, in vitro toxicity tests, for assessing the potential toxicity of new chemical entities are necessary in the early stages of the developmental process, when no information is available about the metabolism or even the target organ toxicity of the compounds to be tested. In vitro specific org...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1010958121396
更新日期:2001-01-01 00:00:00
abstract::Metolachlor, a commonly used herbicide in the Midwestern USA, functions by inhibiting chlorophyll and protein synthesis in target plants. Herbicide exposure has led to detrimental effects in several organisms, affecting their growth and behavior; however, its mechanism of action in nontarget organisms is not yet clear...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9256-z
更新日期:2013-08-01 00:00:00
abstract::Galectin 3 (Gal-3) is upregulated in gastric epithelial cells as a host response to Helicobacter pylori infection. However, the significance of Gal-3 expression in H. pylori-infected cells is not well established. We analyzed Gal-3 intracellular expression, localization, and its effects in H. pylori-infected gastric e...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-016-9315-3
更新日期:2016-02-01 00:00:00
abstract::Ten years have passed since the first publication announcing the generation of induced pluripotent stem cells (iPSCs). Issues related to ethics, immune rejection, and cell availability seemed to be solved following this breakthrough. The development of iPSC technology allows advances in in vitro cell differentiation f...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1007/s10565-016-9377-2
更新日期:2017-06-01 00:00:00
abstract::In order to assess cigarette smoke-induced oxidative damage to intact cells, an assay was developed to measure cell detachment and protection. Due to the complex nature of cigarette smoke, which contains molecules that can interfere with conventional spectrophotometric and fluorometric biochemical assays, transformed ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007478823798
更新日期:1998-08-01 00:00:00
abstract::Oxidative cell injury could be induced by different reactive oxygen species (ROS) operating in multiple pathways. The present work is focused on three different models of oxidative stress: the xanthine/xanthine oxidase system (XXO), an extracellular superoxide anion generator; tert-butylhydroperoxide (TBHP), an analog...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-009-9120-3
更新日期:2010-04-01 00:00:00
abstract::Quinones are widely distributed in the environment, both as natural products and as pollutants. This paper reports that one of the simplest quinones, 2-methyl-1,4-naphthoquinone (menadione), effectively inhibited apoptosis in the presence of UVA. Menadione suppressed the apoptosis induced by serum depletion and cell d...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0089-x
更新日期:2006-09-01 00:00:00
abstract::Glioblastoma (GBM) is the most aggressive type of glioma. Temozolomide (TMZ) is currently the drug of choice used for post-operative chemotherapy of GBM. However, the presence of intrinsic and acquired resistance hinders the success of chemotherapy. To understand the TMZ resistant mechanisms in glioma, we investigated...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-019-09502-7
更新日期:2020-06-01 00:00:00
abstract::Recent studies have demonstrated that the insecticide DDT is a tumor promoting agent. Similar to many other tumor promoting agents, DDT has been shown to inhibit gap junctional intercellular communication (GJIC) between cells in culture, and it has been suggested that DDT-induced loss of communication between adjacent...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249596
更新日期:1990-04-01 00:00:00
abstract::The development of nontoxic but effective radioprotectors is needed because of the increasing risk of human exposure to ionizing radiation. We have reported that alpha-lipoic acid confers considerable radio-protective effect in mouse tissues when given prior to x-irradiation. In the present study, alpha-lipoic acid su...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0137-6
更新日期:2007-03-01 00:00:00
abstract::Considering the recent studies that question previously reported cardio-protective effects of estrogen, there is a growing concern that endocrine disruptors may also contribute to the pathology of cardiovascular disease. PCB153 is one of the most commonly found polychlorinated biphenyls, and based on epidemiological s...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-010-9170-6
更新日期:2011-02-01 00:00:00
abstract::The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater wax moth, Galleria mellonella, with physiologically relevant ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-09448-2
更新日期:2019-06-01 00:00:00
abstract::Ethanol consumption has long been associated with brain damage. However, the mechanism underlying this deleterious effect remains unclear. Among different hypotheses, acetaldehyde is regarded by certain authors as playing a major role in the expression of ethanol toxicity, but there are still some uncertainties about ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-004-0087-9
更新日期:2004-11-01 00:00:00
abstract::The heat-stable enterotoxin (Y-STa) produced by the pathogenic strains of Yersinia enterocolitica is a causative agent of secretory diarrhea. We have reported earlier that Y-STa-induced inositol trisphosphate-mediated cytosolic calcium rise occurs in rat intestinal epithelial cells. In the present communication, the i...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9084-8
更新日期:2009-06-01 00:00:00