Abstract:
:An assay was developed to detect tritium-labeled ovine FSH beta-subunit [( 3H]oFSH beta) secreted from primary ovine pituitary cultures. This procedure used affinity-enriched antibodies raised against reduced and carbamylmethylated oFSH beta (RCM-oFSH beta) in a two-cycle immunoextraction procedure. A discrete species with an apparent mol wt of 21,000 was detected in sodium dodecyl sulfate electrophoretic patterns of immunoextracts from culture medium. This species was identified as RCM-[3H]oFSH beta by its comigration with highly purified RCM-oFSH beta, its reduction in culture media after cultures were treated with 17 beta-estradiol, which normally decreases radioimmunoassayable oFSH; and its displacement from the extracting antibodies by excess unlabeled RCM-oFSH beta. The assay was used in a pulse-chase study to determine that [3H]oFSH beta is secreted within 1-2 h of its synthesis. Prior treatment of cultures with 17 beta-estradiol did not change this timing of secretion.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Whitfield GK,Miller WLdoi
10.1210/endo-115-1-154subject
Has Abstractpub_date
1984-07-01 00:00:00pages
154-9issue
1eissn
0013-7227issn
1945-7170journal_volume
115pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Activin A is a gonadal protein originally isolated from follicular fluid and is recognized as a local regulator of granulosa cell differentiation. Whether activin A promotes folliculogenesis, however, still remains unclarified. The present study was designed to elucidate the effect of activin A on follicular growth in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.11.5526
更新日期:1997-11-01 00:00:00
abstract::Treatment of isolated rat adipocytes with epinephrine or isoproterenol caused a time- and concentration-dependent increase in phospholipid methyltransferase (PLMT) activity that was blocked by propranolol and unaffected by phentolamine. Forskolin mimicked the stimulatory effect on PLMT, and insulin inhibited this effe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2421
更新日期:1987-06-01 00:00:00
abstract::To examine the role of androgen receptor (AR) in Sertoli cells (SC), we used a SC-specific AR knockout (S-AR-/y) mouse to further evaluate the chronological changes of seminiferous tubules and the molecular mechanisms of SC androgen/AR signals on spermatogenesis. Testes morphology began changing as early as postnatal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0138
更新日期:2006-12-01 00:00:00
abstract::While uterine stromal cells (USC) appear to modify the function of uterine epithelial cells (UEC) under certain conditions in vivo, relatively little is known about the effect of epithelial cells on stromal cell differentiation and function. To determine if UEC modulate USC function in vitro, highly enriched (> 95%) c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446600
更新日期:1992-12-01 00:00:00
abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) play potential roles in chemoprevention of colon cancer and others by inhibiting prostaglandin synthesis. In this report, we used LNCaP cells, an androgen-responsive human prostate carcinoma cell line, to study the effects of two NSAIDs, flufenamic acid (FA) and piroxicam ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7246
更新日期:1999-11-01 00:00:00
abstract::Interleukin (IL)-10 is a highly important anti-inflammatory cytokine in the immune system. CD1dhi and CD5+ B cells are both traditionally defined IL-10-secreting B cells. In recent years, a B cell group with combined markers of CD1dhi and CD5+ has been widely studied as it has been reported to suppress autoimmunity in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa033
更新日期:2020-04-01 00:00:00
abstract::In this study, we demonstrate that disruption of neuroendocrine signaling is a major factor driving disease progression in myelin oligodendrocyte glycoprotein-induced chronic relapsing experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Although the initial episode of chronic relapsing ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8292
更新日期:2001-08-01 00:00:00
abstract::In the present study, we report that ACTH induces a transient chloride current. The lack of correlation between ACTH-induced cAMP production and amplitude of the Cl- current, as well as the absence of stimulation by forskolin or 8Br-cAMP indicated that the ACTH-induced current was not cAMP-dependent. We explored the p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.2.7328
更新日期:2000-02-01 00:00:00
abstract::The regression of the tadpole tail is under the direct control of the thyroid hormones and offers a unique system for the study of the action of these hormones. We have examined the binding of L-triiodothyronine (T3) and L-thyroxine (T4) in vitro using tail fin bricks which included epidermal and connective cells. The...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-4-1030
更新日期:1975-10-01 00:00:00
abstract::In granulosa cells derived from medium-sized porcine follicles, certain androgens have been shown to inhibit FSH-stimulated progesterone synthesis. To determine the site at which this inhibition takes place, the effects of androgens on FSH- and (Bu)2cAMP-stimulated pregnenolone and progesterone syntheses were examined...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-6-1999
更新日期:1983-12-01 00:00:00
abstract::The present work extends our previous report that the intracerebroventricular (i.c.v.) injection of interleukin-1beta(IL-1beta, 80 ng) significantly blunted the testosterone response to 1 U/kg human CG (hCG), an effect that we attributed to the stimulation of inhibitory pathways connecting the hypothalamus to the test...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.7.6090
更新日期:1998-07-01 00:00:00
abstract::The effects of L-arginine and its derivatives NG-methyl-L-arginine (Meth-arg) and NG-nitro-L-arginine (Nit-arg) on the insulin release and glucose oxidation of isolated rat islets were investigated to evaluate possible influences of nitric oxide. Also, the insulin release and flow distribution within the perfused rat ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3081
更新日期:1991-06-01 00:00:00
abstract::The objectives of this study were to investigate the presence of immunoreactive GnRH (irGnRH) in scleractinian coral, Euphyllia ancora, study its seasonal variation, and evaluate its biological activity. irGnRH was detected and quantified in coral polyps. The biological activity of coral irGnRH was tested on pituitary...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0584
更新日期:2006-01-01 00:00:00
abstract::Hypoxia, a common stressor in prematurity, leads to sexually dimorphic, short- and long-term effects on the adult hypothalamic-pituitary-adrenal (HPA) and hypothalamic-pituitary-gonadal (HPG) axes. We hypothesized that these effects are due to stress-induced increases in testosterone during early postnatal life. We ev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa085
更新日期:2020-07-01 00:00:00
abstract::Involvement of ionized cytosolic calcium ([Ca2+]i) and protein kinase-C (PKC) in GnRH-stimulated LH release was assessed by correlating measurable changes in [Ca2+]i and LH release in PKC-depleted and nondepleted gonadotropes. Primary cultures of anterior pituitary cells were loaded with the calcium-sensitive fluoresc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547730
更新日期:1992-04-01 00:00:00
abstract::In order to compare, in vitro, the TSH suppressive effects of iodothyronines, rat pituitary quarters were first preincubated with T4, T3, rT3, or 3,3'-diiodothyronine (T2) in Gey and Gey buffer containing 1% bovine serum albumin for 2 h at 37 C and then incubated at 37 C for 1 h with the iodothyronine under study and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-2-393
更新日期:1978-08-01 00:00:00
abstract::GH secretion is markedly blunted in obesity; however, the mechanism(s) mediating this response remains to be elucidated. In the present study we examined the involvement of the two hypothalamic GH-regulatory hormones, GH-releasing factor (GRF) and somatostatin (SRIF), using the genetically obese male Zucker rat. Spont...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3087
更新日期:1990-12-01 00:00:00
abstract::Fasting in rodents is characterized by decreases in serum T4 and T3 levels but no compensatory increase in serum TSH level. The types 1 and 2 deiodinases (D1 and D2) are postulated to play key roles in mediating these changes. However, serum T4 and T3 levels in fasted 5'-deiodinase-deficient mice decreased by at least...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1884
更新日期:2014-08-01 00:00:00
abstract::Liver receptor homolog-1 (LRH-1) is an orphan nuclear receptor that belongs to the NR5A subgroup of nuclear receptors. LRH-1 induces key genes to regulate metabolic process, ovarian function, cancer cell proliferation, and steroidogenesis. In the breast, LRH-1 modulates and synergizes with endogenous estrogen signalin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1948
更新日期:2014-05-01 00:00:00
abstract::In the present study we investigated whether fetal exposure to flutamide affected messenger and protein levels of claudin-11, a key Sertoli cell factor in the establishment of the hemotesticular barrier, at the time of two key events of postnatal testis development: 1) before puberty (postnatal d 14) during the establ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0834
更新日期:2005-03-01 00:00:00
abstract::We have recently reported that pretreatment with electroacupuncture (EA) at stomach meridian point 36 (St36) prevents the chronic cold-stress increase in the hypothalamus-pituitary-adrenal axis (HPA), an action that may be under central control. Given that treatment for stress-related symptoms usually begins after ons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/EN.2015-1018
更新日期:2015-10-01 00:00:00
abstract::In muscular dystrophy there is an imbalance between muscle protein synthesis and protein degradation, resulting in net muscle catabolism and progressive muscle weakness and wasting. Both insulin and insulin-like growth factor I (IGF-I) are known to have an anabolic effect on skeletal muscle, which is believed to be en...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588220
更新日期:1995-11-01 00:00:00
abstract::For insight into transcriptional mechanisms mediating physiological responses to GH, data mining was performed on a profile of GH-regulated genes induced or inhibited at different times in highly responsive 3T3-F442A adipocytes. Gene set enrichment analysis indicated that GH-regulated genes are enriched in pathways in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0212
更新日期:2009-08-01 00:00:00
abstract::IGF binding protein-2 (IGFBP-2) has been implicated in the development and spread of a number of tumor types, and its abrogation in experimental models of cancer is associated with decreased tumor growth. This suggests that targeted inhibition of IGFBP-2 expression in some cancers may have therapeutic benefit. In this...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1335
更新日期:2007-05-01 00:00:00
abstract::The cytokine interleukin-1 (IL-1) has numerous actions in brain, including pronounced neuroendocrine effects. Recent radioligand binding studies have identified high-affinity binding sites for 125I-recombinant human IL-1 alpha in the hippocampus with characteristics similar to those of IL-1 receptors in immune cells. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2666
更新日期:1991-05-01 00:00:00
abstract::This study was designed to investigate the role of dopaminergic mechanisms in the control of aldosterone secretion. Six rhesus monkeys received metoclopramide (1.25 mg, iv), with 5% dextrose (vehicle) or with dopamine (4 micrograms/kg . min) infusions begun 60 min before the administration of the dopamine antagonists....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1173
更新日期:1982-04-01 00:00:00
abstract::Human livers, obtained from donors at the time of transplant, were homogenized in 0.25 M sucrose and fractionated by differential centrifugation. The specific binding of [125I] human (h) GH to total particulate fractions from 18 livers varied from 0.4-5.1% of the total radioactivity/100 micrograms protein. Binding aff...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-4-2167
更新日期:1989-10-01 00:00:00
abstract::Monolayer cultures of mouse adrenal tumor cell line Y-1 have been used to investigate the effects of glucocorticoid on cell replication, [3H]thymidine incorporation into the trichloroacetic acid-precipitated cell fraction, steroidogenesis, and the ACTH receptors of adrenocortical cells. Corticosterone at a concentrati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-2-487
更新日期:1979-02-01 00:00:00
abstract::Agouti-related protein (AgRP) expressed in the arcuate nucleus is a potent orexigenic neuropeptide, which increases food intake and reduces energy expenditure resulting in increases in body weight (BW). Glucocorticoids, key hormones that regulate energy balance, have been shown in rodents to regulate the expression of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1430
更新日期:2016-04-01 00:00:00
abstract::The iodothyronine deiodinases, D1, D2, and D3, all contain selenium (Se) in the form of selenocysteine at their active sites, and they play crucial roles in determining the circulating and intracellular levels of the active thyroid hormone (TH), T3. However, not only are serum T3 levels normal in Se-deficient rats but...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.7.7571
更新日期:2000-07-01 00:00:00