Abstract:
:In rat pancreatic islets, the effect of old age (24-month-old) on [125I]insulin binding, glucose-induced insulin release and inhibition of insulin secretion by exogenous insulin were studied. The results were compared with corresponding data obtained from young (3-month-old) rats. Specifically bound [125I]insulin in islets of old rats was increased by 40% (P less than 0.02) compared to that in young rats. Scatchard plots of displacement studies indicated an increase in receptor number rather than receptor affinity. The insulin-releasing capacity of 16.7 mM glucose did not differ between islets of old and young rats when medium insulin was bound to added antiinsulin serum. In the presence of 16.7 mM glucose (without the addition of antiinsulin serum), insulin secretion was less in islets of old rats compared to that in young rats (283 +/- 38 vs. 528 +/- 29 microU/ml; P less than 0.001). Exogenous insulin inhibited glucose (16.7 mM)-induced insulin release more in islets of old rats than in those of young rats. In conclusion, the present in vitro results may be interpreted to reflect increased insulin binding to islets of aged rats and, consequently, increased inhibition of glucose-mediated insulin secretion due to increased feedback of insulin.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Verspohl EJ,Ammon HPdoi
10.1210/endo-112-6-2147subject
Has Abstractpub_date
1983-06-01 00:00:00pages
2147-51issue
6eissn
0013-7227issn
1945-7170journal_volume
112pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The role of vasopressin (VP) in the regulation of pituitary corticotropin-releasing factor (CRF) receptors was studied by examining the effects of adrenalectomy and VP infusion on pituitary CRF receptors in genetically VP-deficient rats (di/di) and Long-Evans control rats. Binding studies with [125I]Tyr-ovine CRF in 3...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-6-2093
更新日期:1987-12-01 00:00:00
abstract::The effects of electrical and chemical stimulations of the hypothalamus on the plasma levels of glucagon, insulin, and glucose were studied in undisturbed and free-moving rabbits. Electrical stimulation of the ventromedial hypothalamic nucleus (VMH) caused a marked increase in the circulating level of glucagon coincid...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-2-605
更新日期:1981-02-01 00:00:00
abstract::The role of the paraventricular nucleus-dorsal anterior hypothalamus (PVN-DAHA) in the control of anterior pituitary gland secretion of FSH and LH in castrated male and female rats was examined. Bilateral radiofrequency lesions of the PVN-DAHA in chronically ovariectomized (OVX) rats lowered plasma FSH levels by 33% (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2473
更新日期:1984-12-01 00:00:00
abstract::We tested the hypothesis that depressed duodenal calcium absorption in the streptozotocin diabetic rat is the consequence of diabetes rather than nephrotoxicity of the diabetogenic agent causing abnormal renal vitamin D metabolism. We treated streptozotocin diabetic rats with insulin and compared their duodenal calciu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-1-67
更新日期:1977-01-01 00:00:00
abstract::Most of the constitutive hepatic P450 isoforms expressed in the rat exhibit dramatic gender differences. Whereas only male hepatocytes contain CYP2A2, 2C11, and 3A2, only female hepatocytes express CYP2C12 and 3- to 4-fold greater levels of CYP2C7. This sexually dimorphic expression of hepatic P450 isoforms is regulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.3.6545
更新日期:1999-03-01 00:00:00
abstract::Removal of fetuses at day 14 of gestation (Ftx14) in the pregnant rat leads to a marked suppression of serum levels of rat placental lactogen (rPL-II). One might attribute this to compromised placental growth in the absence of a fetus. However, if ovariectomy and fetectomy (Ftx14 Ovx14) are carried out at the same tim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-1-22
更新日期:1984-01-01 00:00:00
abstract::Coordinating the activities of neighboring cells during development in multicellular organisms requires complex cellular interactions involving secreted, cell surface, and extracellular matrix components. Although most cloning efforts have concentrated on secreted molecules, recent work has emphasized the importance o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7196
更新日期:1999-12-01 00:00:00
abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) play potential roles in chemoprevention of colon cancer and others by inhibiting prostaglandin synthesis. In this report, we used LNCaP cells, an androgen-responsive human prostate carcinoma cell line, to study the effects of two NSAIDs, flufenamic acid (FA) and piroxicam ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7246
更新日期:1999-11-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) is a multifunctional cytokine that is thought to play a major role in the regulation of growth and differentiation of thyroid cells. However, little is known of its detailed mechanisms of action in thyrocytes. We have therefore studied the molecular mechanisms of TGF-beta1 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7918
更新日期:2001-01-01 00:00:00
abstract::Pituitary growth hormone (GH) and insulinlike growth factor (IGF)-1 are anabolic hormones whose physiological roles are particularly important during development. The activity of the GH/IGF-1 axis is controlled by complex neuroendocrine systems including two hypothalamic neuropeptides, GH-releasing hormone (GHRH) and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03175
更新日期:2018-04-01 00:00:00
abstract::A thyroid transcription factor-1 (TTF-1)-binding element in the rat TSH receptor (TSHR) promoter, between -189 and -175 basepairs (bp), is important for both thyroid-specific expression and thyroid-specific TSH/cAMP autoregulation of the TSHR. The identification of an up-stream TTF-1-binding site and its relationship ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828540
更新日期:1995-01-01 00:00:00
abstract::Insulin-like growth factor-I (IGF-I) is produced in multiple tissues and is believed to exert its action in a paracrine or autocrine fashion. We have investigated the GH dependency of tissue IGF-I gene expression in rats harboring implanted somatomammotropic tumors. Circulating GH and radioimmunoassayable IGF-I levels...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-2204
更新日期:1988-05-01 00:00:00
abstract::Daily nocturnal (N) and diurnal (D) surges of PRL are characteristic of a pseudopregnancy (psp) induced by cervical stimulation (CS). These semicircadian surges persist for 13-14 days, after which time they cease, and psp ends. The purpose of this study was to describe the involvement of the ovarian steroids in the ma...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-4-1293
更新日期:1981-04-01 00:00:00
abstract::Flavonoids are functional constituents of many fruits and vegetables. Some flavonoids have antidiabetic properties because they improve altered glucose and oxidative metabolisms of diabetic states. Procyanidins are flavonoids with an oligomeric structure, and it has been shown that they can improve the pathological ox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0764
更新日期:2004-11-01 00:00:00
abstract::We have established a system, the CA77 rat medullary thyroid carcinoma cell line, for studying the products of somatostatin (SS) gene expression. Based on the amino acid sequence of proSS, we developed a RIA for the amino terminus of proSS (proSS-NTP) and demonstrated in acidic cell extracts two major proSS-NTP-contai...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-218
更新日期:1986-01-01 00:00:00
abstract::The secretory pattern of GH secretion is markedly sexually dimorphic in the adult rat. The patterning of GH secretion is determined by the coordinated activity of somatostatin (SS)- and GH-releasing hormone (GHRH)-containing neurosecretory cells located in the hypothalamus. In this study we examined whether there is s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2369
更新日期:1991-05-01 00:00:00
abstract::Retinoid X receptor (RXR) occupies a central position within the nuclear receptor superfamily, serving as an obligatory partner to numerous other nuclear receptors, including vitamin D receptor (VDR). In the current study, we examined whether phosphorylation of RXRα at serine 260 affects VDR/RXR and VDR interacting pr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03098
更新日期:2018-03-01 00:00:00
abstract::We have found notable sex difference in peptidylarginine deiminase (EC 3.5.3.15) in rat pituitaries. Pituitaries collected from 3-week to 6-month-old male rats, and those from 3-week-old females showed negligible enzyme activities. The enzyme activity of female pituitaries increased markedly by 2 months, and even furt...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-2666
更新日期:1989-06-01 00:00:00
abstract::Tear fluid is known to contain many different hormones with relevance for ocular surface homeostasis. We studied the presence and functional role of insulin-like factor 3 (INSL3) and its cognate receptor RXFP2 (relaxin/insulin-like family peptide receptor 2) at the ocular surface and in tears. Expression of human INSL...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2201
更新日期:2013-06-01 00:00:00
abstract::The concentration of testosterone (T) and 5alpha-dihydrotestosterone (DHT) in plasma, seminal fluid, and epididymis of male Wistar rats have been determined by radioimmunoassay. The DHT/T ratio rose from 0.1 in blood to 1 in testicular fluid and 4 in epididymal fluid. This ratio is 2 in tissues of "caput" epididymis a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-1-111
更新日期:1976-01-01 00:00:00
abstract::T(4) activation into T(3) is catalyzed by type 2 deiodinase (D2) in the brain. The rapid induction of D2 in astrocytes by transient brain ischemia has prompted us to explore the effects of hypoxia on D2 in cultures of astrocytes. Hypoxia (2.5% O(2)) of cultured astrocytes increased D2 activity, alone or in association...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0625
更新日期:2007-10-01 00:00:00
abstract::The absence of TSH-stimulated radioiodide uptake in differentiated thyroid cancer is associated with a high recurrence rate and reduced survival. We studied regulation of the sodium/iodide symporter gene in human papillary thyroid cancer cell lines (BHP) and primary human thyroid cells. BHP cells expressed very low le...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8344
更新日期:2001-08-01 00:00:00
abstract::alpha-Fetoprotein (AFP) is present in high levels in fetal fluids, certain neoplasias, and regenerating liver. Although AFP's physiological role remains an enigma, we have recently demonstrated mitogenic activity for AFP. Using a primary monolayer culture system, we have further investigated the proliferative activity...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-1-217
更新日期:1991-07-01 00:00:00
abstract::To study blood capillary growth in the hyperplastic thyroid gland, rats were fed 0.25% thiouracil in a low iodine diet for time intervals up to 100 days. Thyroids were fixed by vascular perfusion and embeded in Epon. Whole lobes were sectioned from pole to pole and slides were prepared of sections every 0.3 mm. Capill...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-2306
更新日期:1978-12-01 00:00:00
abstract::Studies in the rat and rabbit indicate that facilitatory effects of neuropeptide Y (NPY) as well as norepinephrine (NE) on LH and LHRH release are dependent on the presence of the ovarian steroid estrogen. However, we have previously found the NE and an alpha-1-adrenergic agonist are both stimulatory to pulsatile LHRH...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-1144
更新日期:1991-02-01 00:00:00
abstract::Epidemiological evidence indicates that phytoestrogens inhibit cancer formation and growth, reduce cholesterol levels, and show benefits in treating osteoporosis. At least some of these activities are mediated through the interaction of phytoestrogens with estrogen receptors alpha and beta (ERalpha and ERbeta). Resver...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.10.7721
更新日期:2000-10-01 00:00:00
abstract::Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. Gremlin, a BMP antagonist, is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. However, its effects in vivo are not known. To investigate the actions of gremlin on bone remodeling in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0766
更新日期:2005-02-01 00:00:00
abstract::Two different types of corticoid receptor molecules bind circulating corticosterone in brain: mineralocorticoid receptors (MR) and glucocorticoid receptors. MR exhibit the highest affinity for the endogenous glucocorticoid in the rat, corticosterone. During development, low corticosterone levels influence neurogenesis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.7.6095
更新日期:1998-07-01 00:00:00
abstract::Experimental evidence indicates that the neonatal gonadal steroid environment is an important determinant of the sexual dimorphism of GH secretion and body growth. However, the influence of the sex steroids in GH control during adult life and their mechanism/site of action are largely unknown. In the present study we ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1345780
更新日期:1992-01-01 00:00:00
abstract::Although GH plays a key role in postnatal growth, prenatal growth is thought to be GH independent. However, recent data has shown GH receptor/binding protein (GHR/BP) to be present in rat fetal tissues as early as fetal stage E12. The aim of the present study was to investigate tissue-specific production of the GHR/BP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7664680
更新日期:1995-10-01 00:00:00