Abstract:
:We tested the hypothesis that depressed duodenal calcium absorption in the streptozotocin diabetic rat is the consequence of diabetes rather than nephrotoxicity of the diabetogenic agent causing abnormal renal vitamin D metabolism. We treated streptozotocin diabetic rats with insulin and compared their duodenal calcium transport response with that of untreated diabetics and matched controls. Insulin treatment restored depressed calcium transport of diabetics to control levels in in vivo studies and significantly increased calcium transport in vitro. Previous studies showed that even in uncontrolled diabetes the mucosa retains the ability to respond to an end organ stimulus enhancing calcium transport: 1,25-dihydroxycholecalciferol corrects the defect, but vitamin D and 25-hydroxycholecalciferol are ineffective. Since 1,25-dihydroxycholecalciferol is synthesized in the kidney, these findings, in conjunction with the current study, are consistent with the association of experimental diabetes with a renal defect depressing synthesis of 1,25-dihydroxycholecalciferol. Since insulin treatment restores duodenal calcium transport, the renal defects is unlikely to be caused by streptozotocin nephrotoxicity.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Schneider LE,Nowosielski LM,Schedl HPdoi
10.1210/endo-100-1-67subject
Has Abstractpub_date
1977-01-01 00:00:00pages
67-73issue
1eissn
0013-7227issn
1945-7170journal_volume
100pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Binding of lactogenic hormones to particulate and solubilized microsomal membranes isolated from mammary glands of lactating mice is inhibited by direct addition of 10(-10) M or greater concentrations of triphenylethylene antiestrogens [i.e. tamoxifen (TAM), 4-hydroxy-tamoxifen, and Nafoxidine] to the binding assays. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-532
更新日期:1991-01-01 00:00:00
abstract::In this study on highly enriched populations of cultured rat corticotropes, Ca2+ channel inhibitors were used to identify subtypes of the high threshold Ca2+ channel current under voltage clamp conditions. From a holding potential (-50 mV) that eliminated the low threshold T-type current, 52 +/- 4% of the total curren...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.9.7649100
更新日期:1995-09-01 00:00:00
abstract::We constructed seven chimeric molecules in which sequential segments in the cDNA for thyroid peroxidase (TPO) were replaced with the homologous regions of myeloperoxidase (MPO) cDNA. The sizes of the translated cDNA segments A through G ranged from 23-175 amino acid residues in length. The TPO-MPO cDNA chimeras, inser...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243270
更新日期:1993-12-01 00:00:00
abstract::Decidualization, the process by which fibroblastic human endometrial stromal cells (HESC) differentiate into secretory decidual cells, is a critical event during the establishment of pregnancy. It is dependent on the steroid hormone progesterone acting through the nuclear progesterone receptor (PR). Previously, we ide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqz021
更新日期:2020-01-01 00:00:00
abstract::The PTH and PTH-related protein (PTHrP) system consists of two hormones, at least two G protein-coupled seven-transmembrane domain receptors, and at least two intracellular signal transduction pathways for each receptor. The PTH/PTHrP receptor is present in the conventional target tissues of PTH action, namely kidney ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.10.8828480
更新日期:1996-10-01 00:00:00
abstract::A biologically active form of the epidermal growth factor (EGF) precursor has been detected in human fluids and secretions. The secreted protein identified in human urine and milk has an apparent molecular mass of 160-170 kilodaltons and exhibits an affinity for the glycosaminoglycan heparin. More importantly, the sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8419140
更新日期:1993-01-01 00:00:00
abstract::In the present study, steroidogenesis in two different Leydig tumor cell lines was compared. One, the MA-10 mouse tumor cell line, produces steroids and the steroidogenic acute regulatory (StAR) protein only when stimulated by trophic hormones and cAMP analogs. The other, the R2C rat tumor cell line, produces steroids...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0941
更新日期:2004-12-01 00:00:00
abstract::The rapid, nongenomic effects of estrogen are increasingly recognized as playing an important role in several aspects of estrogen action. Rapid activation of the mitogen-activated protein kinase (MAPK) signaling pathway by estrogen is among the more recently identified of these effects. To explore the role of estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.6.8071
更新日期:2001-06-01 00:00:00
abstract::The lactogen-dependent rat Nb2 lymphoma is a useful model to investigate PRL signaling pathways that lead to regulation of gene transcription. A primary mechanism coupled to PRL receptor (PRLR) activation in Nb2 cells involves phosphorylation by Jak-family tyrosine kinases of one or more signal transducers and activat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7215
更新日期:1999-12-01 00:00:00
abstract::Flow cytometry was used to separate and identify Sertoli and germ cell populations in primary rat testicular cultures derived from animals of different ages on the basis of cell size and DNA and lipid content. Multiparameter fluorescent evaluation of each cell preparation resulted in the assignment of specific stainin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.2.1310280
更新日期:1992-02-01 00:00:00
abstract::We have compared the physicochemical properties of rat uterine nuclear estrogen receptors (ER) labeled with (E2) or the high affinity antiestrogen 4-hydroxytamoxifen [OH Tam; 1-[4-(2-dimethylaminoethoxy)phenyl]-1-(4-hydroxyphenyl) 2-phenyl-but-1-(Z)ene]. Nuclear ER labeled in vivo with either ligand had sedimentation ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-904
更新日期:1986-08-01 00:00:00
abstract::The lipolysis, ketosis and hypertriglyceridemia of fasted rats in late pregnancy may be related to decreased insulin secretion geared to decreased glucose availability. In odd-carbon fatty acid enriched (OCE) rats, the odd-carbon fatty acids (OCFA) mobilized during fasting provide terminal three-carbon residues which ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-373
更新日期:1977-02-01 00:00:00
abstract::Neuronal remodeling in response to deafferenting lesions in the brain can be enhanced by estradiol (E2). Astrocytes are among the targets of E2 in complex interactions with neurons and may support or inhibit neuronal remodeling. In ovariectomized female rats given entorhinal cortex lesions, E2 replacement inhibited th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.2.8615
更新日期:2002-02-01 00:00:00
abstract::Electrical stimulation of the preoptic area resulted in a significant increase in the secretion rate of immunoreactive somatostatin (IRS) and its concentration in the hypophysial portal blood of urethane-anesthetized rats. In contrast, stimulation of the ventromedial nucleus did not cause any changes in portal immunor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-6-1416
更新日期:1979-12-01 00:00:00
abstract::Certain metabolic properties of hormonally responsive osteogenic sarcoma cells derived from a transplantable rat tumor have been compared with those of related normal rat bone cells. All studies were carried out on cells grown in monolayer culture. Normal rat bone cells derived by repeated collagenase/trypsin digestio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-213
更新日期:1981-01-01 00:00:00
abstract::GH is secreted in a pulsatile manner, the pattern of which plays an important role in the regulation of growth and metabolism. Sex steroids are also known to participate in metabolic regulation. The present study was undertaken to elucidate the relationship between changes in GH pulsatility and metabolic transition du...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1434
更新日期:2005-06-01 00:00:00
abstract::The CYP11A1 encodes cytochrome P450scc, catalyzing the first step of steroidogenesis in adrenals and gonads under the control of cAMP-mediated hormonal signals. The cAMP-induced activation of human CYP11A1 has been suggested to depend on the transcription factor cAMP-responsive element-binding protein (CREB), but the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0938
更新日期:2007-04-01 00:00:00
abstract::The effect of constant infusions of bovine TSH in different concentrations on the pattern of T4 and T3 secretion was examined in perfused canine thyroids isolated in situ. Each of the thyroid lobes was isolated and perfused in each animal. It was thus possible to examine the effect of two different concentrations of T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-656
更新日期:1977-03-01 00:00:00
abstract::We have recently demonstrated that GnRH-stimulated gonadotropin secretion is augmented by coadministration with neuropeptide-Y (NPY) in anterior pituitaries removed in the afternoon from proestrous, but not metestrous, rats. To test the hypothesis that these effects of NPY are due to an interaction with progesterone (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828530
更新日期:1995-01-01 00:00:00
abstract::The role of PRL in mammalian salt and water balance remains controversial. To avoid the methodological problems of exogenous PRL administration, endogenous hyperprolactinemia was established in normal rats by implantation of extra anterior pituitary glands under the kidney capsule. To eliminate excess glucocorticoid s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1519
更新日期:1986-04-01 00:00:00
abstract::It has been postulated that the rate of hepatic very low density lipoprotein (VLDL) apolipoprotein (apo) B secretion is dependent upon the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase. To test this hypothesis in vivo, apoB kinetic studies were carried out in miniature pigs before and after 21 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7150
更新日期:1999-11-01 00:00:00
abstract::The intraislet insulin hypothesis has been proposed to explain absent glucagon responses to hypoglycemia. Recently we directly confirmed this hypothesis by restoring glucagon secretion via provision of a pancreatic artery insulin infusion, which was switched off at the time of hypoglycemia in Wistar rats made diabetic...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1375
更新日期:2007-06-01 00:00:00
abstract::Brown adipose tissue (BAT) thermogenesis is activated by the sympathetic nervous system. BAT responses to norepinephrine are blunted in hypothyroidism and are rapidly restored by thyroid hormone. We examined in rats the effects of thyroid hormone on BAT beta 1- and beta 2-adrenergic receptors (AR) expression and capac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628360
更新日期:1995-08-01 00:00:00
abstract::One day of fasting suppresses pulsatile LH secretion in adult male rhesus monkeys. In the present study, we determined whether the hypothalamic-pituitary-adrenal (HPA) axis is activated by 1 day of fasting, and if there is any evidence that an increase in the activity of the HPA axis causes the fasting-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8389280
更新日期:1993-06-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) and interleukin-1 beta (IL-1) are secreted by activated monocytes and other immune cells. Since IL-1 has been shown to elevate rat plasma ACTH and both of these cytokines induce similar acute-phase responses, the present studies of TNF were undertaken to characterize the ACTH response...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3131
更新日期:1989-06-01 00:00:00
abstract::A study was conducted to determine the role of the thyroid gland in three neuroendocrine responses to photoperiod; secretion of melatonin, PRL, and LH. Ewes were thyroidectomized (THX) in midsummer or left thyroid intact, and both groups were moved indoors to artificial short days (8 h of light, 16 h of darkness) for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013340
更新日期:1994-07-01 00:00:00
abstract::To examine the physiological effects of norepinephrine (NE) in the paraventricular nucleus of the hypothalamus (PVH) on CRF gene expression and CRF release, NE was microinjected bilaterally into the PVH of conscious rats, and kinetic studies were performed on the levels of POMC messenger RNA (mRNA) in the anterior pit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.5.7956940
更新日期:1994-11-01 00:00:00
abstract::The GT1 GnRH cell lines express all three subunits of the cyclic nucleotide-gated (CNG) channels (CNG2, 4.3 and 5) expressed in olfactory neurons. We investigated using in situ hybridization and double immunofluorescence whether endogenous GnRH neurons in therat also express CNG channel subunits. Sections from male an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8951
更新日期:2002-06-01 00:00:00
abstract::Estrogens are crucial for growth and function of the female genital tract. Recently, we showed that induction of uterine epithelial proliferation by estradiol is a paracrine event requiring an estrogen receptor-positive stroma. Growth factors [such as EGF (epidermal growth factor) ligands] are likely paracrine mediato...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5817
更新日期:1998-03-01 00:00:00
abstract::GH3 rat pituitary cells have L-type voltage-gated calcium channels, and both the rate of uptake of 45Ca2+ and the concentration of intracellular free calcium ion ([Ca2+]i) are increased by depolarization with high potassium. Cells incubated for several days with 10 nM epidermal growth factor (EGF) responded to depolar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.7686480
更新日期:1993-07-01 00:00:00