Abstract:
:Estrogens are crucial for growth and function of the female genital tract. Recently, we showed that induction of uterine epithelial proliferation by estradiol is a paracrine event requiring an estrogen receptor-positive stroma. Growth factors [such as EGF (epidermal growth factor) ligands] are likely paracrine mediators, which may directly or indirectly regulate epithelial proliferation in estrogen target organs via cell-cell interactions. In this report, we used mice with a null mutation in their EGF receptor (EGFR) to examine the role of EGFR signaling in growth of the uterus and vagina and in estrogen-induced uterine and vaginal epithelial proliferation. When WT and EGFR-knockout (EGFR-KO) uteri and vaginae were grown as renal capsule grafts in nude mice, growth of uterine and vaginal grafts of EGFR-KO mice was reduced, compared with their WT counterparts. Grafts of both EGFR-KO uteri and vaginae were about one third smaller (wet weight) than their corresponding WT organs, even though differentiation of both epithelium and mesenchyme were normal in both cases. Both wild-type and EGFR-KO vaginal grafts contained within their lumina alternating layers of cornified and mucified epithelial cell layers, indicating cyclic alteration of epithelial differentiation. In response to estradiol treatment, stromal cell labeling index (LI), as assessed by incorporation of 3H-thymidine, was severely depressed in EGFR-KO uterine and vaginal grafts vs. stromal cell LI in WT uterine and vaginal grafts. Unexpectedly, epithelium of both EGFR-KO and wild-type grafts responded comparably to estradiol with a marked elevation (approximately 7-fold overall) of epithelial LI in response to estradiol in uterine and vaginal epithelia. These data supported the hypothesis that overall uterine and vaginal organ growth, in response to estrogen, required EGFR signaling for DNA synthesis in the fibromuscular stroma, whereas EGFR signaling was not essential for estrogen-induced epithelial growth in the uterus and vagina.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Hom YK,Young P,Wiesen JF,Miettinen PJ,Derynck R,Werb Z,Cunha GRdoi
10.1210/endo.139.3.5817subject
Has Abstractpub_date
1998-03-01 00:00:00pages
913-21issue
3eissn
0013-7227issn
1945-7170journal_volume
139pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Heart failure is a leading cause of death, especially in the elderly or obese and diabetic populations. Various remodeling events have been characterized, which collectively contribute to the progression of heart failure. Of particular interest, autophagy has recently emerged as an important determinant of cardiac rem...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1162
更新日期:2015-07-01 00:00:00
abstract::Androgens are required for the development and function of the prostate. In a normal human prostate, androgens control the synthesis of proteins such as prostate-specific antigen and human glandular kallikrein. The prostate secretes these proteins as well as a number of other compounds to form the prostatic fluid. Usi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5926
更新日期:1998-04-01 00:00:00
abstract::Mouse β-cell-specific reporter lines have played a key role in diabetes research. Although the rat provides several advantages, its use has lagged behind the mouse due to the relative paucity of genetic models. In this report we describe the generation and characterization of transgenic rats expressing a Renilla lucif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1550
更新日期:2016-03-01 00:00:00
abstract::Thyroid toxicity of iodide excess has been demonstrated in animals fed with an iodide-rich diet; in vitro iodide is cytotoxic, inhibits cell growth, and induces morphological changes in thyroid cells of some species. In this study, we investigated the effect of iodide excess in an immortalized thyroid cell line (TAD-2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.2.7291
更新日期:2000-02-01 00:00:00
abstract::Previous studies have established the presence of an extrahypothalamic GnRH in a variety of tissues. GnRH receptor is known to be present in the placenta, which produces and secretes the decapeptide from the very early stages of placentation. We hypothesized that GnRH may play a role in the preimplantation development...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6899
更新日期:1999-08-01 00:00:00
abstract::Specific triiodothyronine receptor activity has been detected in rat lung nuclei isolated by Triton X-100, which was sensitive to pH and exhibited temperature-dependent association kinetics. This activity represented a single order of high affinity binding sites, with an apparent dissociation constant for 3,3',5-triio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-5-1628
更新日期:1978-05-01 00:00:00
abstract::The distribution of androgen receptor expression within the developing rat gubernaculum was examined at different times during fetal and neonatal development using polyclonal antibodies directed at the amino-terminus (amino acids 1-21) and the carboxy-terminus (amino acids 898-917) of the androgen receptor. These stud...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-383
更新日期:1991-01-01 00:00:00
abstract::Glucose-dependent insulinotropic peptide (GIP) is a 42-amino acid peptide synthesized and secreted from endocrine cells in the small intestine. The role of GIP in coupling nutrient intake and insulin secretion, the incretin effect, is well known. We report that GIP receptor messenger RNA and protein are present in nor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.3.7366
更新日期:2000-03-01 00:00:00
abstract::Ghrelin, a novel GH-releasing acylated peptide, was recently isolated from rat stomach. It stimulated the release of GH from the anterior pituitary through the GH secretagogue receptor (GHS-R). Ghrelin messenger RNA and the peptide are present in rat stomach, but its cellular source has yet to be determined. Using two...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7757
更新日期:2000-11-01 00:00:00
abstract::The effects of food restriction of the mother (65% restriction of ad libitum food intake) on fetal and maternal insulin secretion and islet function were studied at 21 days gestation in three different rat populations: 1) undernourished from 0-7 days gestation, 2) undernourished from 7-14 days gestation, and 3) undern...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.6.5202
更新日期:1997-06-01 00:00:00
abstract::Hypothalamic insulin signaling is essential to the maintenance of glucose and energy homeostasis. During pathological states, such as obesity and type 2 diabetes mellitus, insulin signaling is impaired. One key mechanism involved in the development of insulin resistance is lipotoxicity, through increased circulating s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1122
更新日期:2010-02-01 00:00:00
abstract::Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ET(A)R) in murine models of thyroid carcinoma using optical imaging methods. A...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-2017
更新日期:2012-06-01 00:00:00
abstract::We are investigating the mechanisms by which aromatic hydrocarbons, such as 3-methylcholanthrene (MC), suppress hepatic cytochrome P450 2C11 (CYP2C11) gene expression. CYP2C11 is an enzyme expressed in the liver of male rats and is regulated by a pulsatile pattern of GH secretion. We have previously shown that MC atte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220212
更新日期:2002-09-01 00:00:00
abstract::Nuclear proteins extracted from purified nuclei with 0.4M KCl at pH 7.4 OR 8.5 are able to bind L-triiodothyronine (T3) giving rise to nuclear thyroid hormone binding protein-T3 (NTBP-T3) complexes. Binding is maximum in 3 h at 20 C. It is thermolabile even at 36 C, inhibited by p-hydroxymercuribenzoate and markedly e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-5-1201
更新日期:1975-05-01 00:00:00
abstract::Recent studies using twins and inbred strains of mice reveal evidence for genetic mechanisms contributing to variation in circulating levels of IGF-I, IGF-II, and IGF binding protein (IGFBP)-3. To examine the hypothesis that serum IGFBP-5 levels have a strong heritable component, we intercrossed two inbred strains of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0042
更新日期:2003-08-01 00:00:00
abstract::Early postnatal hyperalimentation has long-term implications for obesity and developing renal disease. Suppressor of cytokine signaling (SOCS) 3 inhibits phosphorylation of signal transducer and activator of transcription (STAT) 3 and ERK1/2 and thereby plays a pivotal role in mediating leptin resistance. In addition,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1670
更新日期:2012-03-01 00:00:00
abstract::Estrogens stimulate cell proliferation in typical estrogen-responsive tissues including the anterior pituitary gland. Here we report that 17-beta estradiol (E2) has estrogen receptor-mediated mitogenic and antimitogenic actions on rat lactotrophs in primary culture, depending on the cell context. E2 did not affect bas...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.7.8915
更新日期:2002-07-01 00:00:00
abstract::Reactive oxygen species, such as superoxide, hydrogen peroxide, and lipid peroxides, impair luteal function. Vitamin E, a lipophilic antioxidant vitamin, provides a major avenue of protection by scavenging free radicals and terminating lipid peroxidation. We previously showed that ovarian vitamin E levels increase aft...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.2.8033800
更新日期:1994-08-01 00:00:00
abstract::Serum levels of progesterone in hysterectomized gilts were determined to evaluate the effects of exogenous 17 beta-estradiol (17 beta-E2) on the secretory activity of aging corpora lutea. Gilts were hysterectomized on day 6 of the estrous cycle and given im injections of 17 beta-E2 to mimic blood levels of endogenous ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-5-1876
更新日期:1984-11-01 00:00:00
abstract::The possible role of atrial natriuretic factor (ANF) in the regulation of adrenal sensitivity to angiotensin II (AII) was investigated in vivo and in vitro by analyzing the characteristics of the inhibitory effect of ANF on aldosterone production stimulated by AII and other stimuli. In isolated adrenal glomerulosa cel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-1-299
更新日期:1987-01-01 00:00:00
abstract::CD36/FAT (fatty acid translocase) is associated with human and murine nonalcoholic fatty liver disease, but it has been unclear whether it is simply a marker or whether it directly contributes to disease pathogenesis. Mice with hepatocyte-specific deletion of Janus kinase 2 (JAK2L mice) have increased circulating free...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1866
更新日期:2016-02-01 00:00:00
abstract::To determine if CRH and glucocorticoids are respectively required for hypophagia and catabolism in malnutrition, we have subjected wild-type (WT) and CRH knockout (KO) mice to dietary protein deprivation. Compared with WT mice, CRH KO mice exhibited greater decreases in food intake and negligible change in plasma cort...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6910
更新日期:1999-08-01 00:00:00
abstract::Wortmannin (5-100 nM), a specific phosphatidyinositol 3-kinase inhibitor, augmented 8 mM glucose-induced insulin secretion from control Sprague Dawley rat islets in a dose-dependent manner. This effect persisted after its removal from the perifusion medium; however, this augmenting effect was reduced by the calcium ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.9.7636
更新日期:2000-09-01 00:00:00
abstract::Kisspeptin neurons play an essential role in the regulation of fertility through direct regulation of the GnRH neurons. However, the relative contributions of the two functionally distinct kisspeptin neuron subpopulations to this critical regulation are not fully understood. Here we analyzed the specific projection pa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1131
更新日期:2015-07-01 00:00:00
abstract::Hormone-dependent breast cancers respond to inhibitors of estrogen synthesis or action with tumor regression and with a reduction of new metastases. The mechanisms underlying the effects of estrogen on metastasis likely differ from those on tumor regression. Cell migration is a key first step in the metastatic process...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1506
更新日期:2010-11-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta) is a multifunctional growth factor in bone that is secreted as a latent complex and must be activated in order to influence cellular activity. We have investigated the influence of dexamethasone (Dex; a potent glucocorticoid) on the secretion and activation of latent TGF beta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404670
更新日期:1993-11-01 00:00:00
abstract::Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-depe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-5-2249
更新日期:1986-11-01 00:00:00
abstract::Activin A is a member of the transforming growth factor-beta family of growth factors and a potent regulator of cellular activity. A number of binding proteins for activin A have been identified, including alpha 2-macroglobulin (alpha 2M). Alpha 2M has several conformational states that are known to have different gro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588280
更新日期:1995-12-01 00:00:00
abstract::As we previously showed, we have synthesized a new family of 17β-estradiol-platinum(II) hybrids. Earlier studies revealed the VP-128 hybrid to show high efficiency compared with cisplatin toward hormone-dependent breast cancer cells. In the present research, we have studied the antitumor activity of VP-128 in vitro an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1083
更新日期:2013-07-01 00:00:00
abstract::The pancreatic polypeptide cell, the only mature endocrine cell in the fetal pig pancreas, produces equimolar amounts of two peptides, pancreatic polypeptide and pancreatic icosapeptide, from the same precursor. The amino acid sequence of pancreatic polypeptide is more homogeneous among species, whereas pancreatic ico...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.10.8409
更新日期:2001-10-01 00:00:00