Abstract:
:The distribution of androgen receptor expression within the developing rat gubernaculum was examined at different times during fetal and neonatal development using polyclonal antibodies directed at the amino-terminus (amino acids 1-21) and the carboxy-terminus (amino acids 898-917) of the androgen receptor. These studies reveal a high level expression of androgen receptor in the undifferentiated cells that comprise the mesenchymal core of the gubernaculum in early development. However, during the morphological alterations of the gubernaculum into a muscular structure, the level of androgen receptor detected in these cells declines, with minimal immunoreactivity present 2 weeks postpartum. Whether the loss of staining of the mesenchyme is related to decreases in androgen receptor expression, death of the mesenchymal cells, or differentiation of these cells into myoblasts remains to be determined.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Husmann DA,McPhaul MJdoi
10.1210/endo-128-1-383subject
Has Abstractpub_date
1991-01-01 00:00:00pages
383-7issue
1eissn
0013-7227issn
1945-7170journal_volume
128pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Exposure of FRTL-5 cells to iodide (I-) in excess of 3 microM suppresses the concentrative uptake of I-. The depression of I- uptake measured at the steady state is due to decrease in the rate of I- influx and not to an effect on I- efflux. Exposure to NaI is associated with decreased T4 secretion and also depressed N...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2477
更新日期:1986-06-01 00:00:00
abstract::Aldosterone selectivity in mineralocorticoid target tissues has been suggested to be due to 11 beta-hydroxysteroid dehydrogenase (11-OHSD), which, by inactivating the endogenous glucocorticoids cortisol and corticosterone (CS), would allow aldosterone to bind to the mineralocorticoid receptor that has equal affinity f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-1-17
更新日期:1991-07-01 00:00:00
abstract::The CYP11A1 encodes cytochrome P450scc, catalyzing the first step of steroidogenesis in adrenals and gonads under the control of cAMP-mediated hormonal signals. The cAMP-induced activation of human CYP11A1 has been suggested to depend on the transcription factor cAMP-responsive element-binding protein (CREB), but the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0938
更新日期:2007-04-01 00:00:00
abstract::Metastasis of various malignant cells is inversely related to the abundance of the Nm23-H1 protein. The role of estrogens in tumor metastasis has now been investigated by examining the effect of E2 on the expression of the Nm23-H1 gene. Three human breast carcinoma cell lines, in which endogenous ERalpha is expressed ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.2.8620
更新日期:2002-02-01 00:00:00
abstract::The effects of short term (1-month) and life-long 60% ad libitum food restriction on the adrenocortical response to restraint stress were compared in young and aged Fischer 344 rats. In rats restricted for 1 month (study 1), the adrenocortical response differed as a function of age. In 8-month-old animals, the initial...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-4-1934
更新日期:1988-10-01 00:00:00
abstract::In the mouse, there is a large family of paralogous genes closely related to PRL. The objective of this report was to investigate the organization of the mouse PRL gene family locus. PRL family genes reside on chromosome 13 of the mouse genome. The PRL gene family members were localized to a series of overlapping bact...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220724
更新日期:2003-01-01 00:00:00
abstract::Developmental effects of genistein (Gen) on the mammary gland were investigated using outbred female CD-1 mice treated neonatally on d 1-5 by sc injections at doses of 0.5, 5, or 50 mg/kg.d. Examination of mammary gland whole mounts (no. 4) before puberty (4 wk) revealed no morphological differences in development aft...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0389
更新日期:2006-10-01 00:00:00
abstract::An ovarian thecal/interstitial cell preparation was obtained by enzymatic digestion of immature rat ovaries depleted of granulosa cells. A component in thecal/interstitial cell-conditioned medium inhibited specific binding of [125I]iodo-epidermal growth factor ([125I]EGF) to its cell surface receptor, as determined wi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1892
更新日期:1987-11-01 00:00:00
abstract::Congenital adrenal hyperplasia is a group of common inherited disorders leading to glucocorticoid deficiency. Most cases are caused by 21-hydroxylase deficiency (21OHD). The systemic consequences of imbalanced steroid hormone biosynthesis due to severe 21OHD remains poorly understood. Therefore, we developed a zebrafi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00549
更新日期:2017-12-01 00:00:00
abstract::Down regulation of insulin receptor number by insulin at physiological concentrating (10(-9) M) has been demonstrated in primary cultures of adult rat liver parenchymal cells in monolayer. However, because insulin is rapidly degraded in this culture system, special efforts are necessary to maintain the concentration o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-2-548
更新日期:1978-08-01 00:00:00
abstract::It has previously been established that neuropeptide-Y (NPY) is a potent inhibitor of alpha MSH release from the pars intermedia of the amphibian Xenopus laevis. The location of binding sites for NPY in the pars intermedia of the pituitary has now been studied with light microscopic autoradiography, using a dispersed ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-735
更新日期:1991-02-01 00:00:00
abstract::Although LHRH can stimulate the release of both LH and FSH from the pituitary, there are a number of instances in which the secretion of LH and FSH are divergent. Previous studies from our laboratory have indicated that pulsatile LH and FSH secretion are independently regulated by gonadal factors. We have, therefore, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-609
更新日期:1986-02-01 00:00:00
abstract::Using a Leydig tumor cell line (MA-10) transiently transfected with the human lutropin receptor (hLHR) and mutants thereof, we examined the identity of the G proteins activated by the agonist-engaged hLHR-wild type (wt) and by three of its naturally occurring constitutively active mutants. Two of the mutants examined,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0365
更新日期:2003-09-01 00:00:00
abstract::Iodine deficiency (ID) induces TSH-independent microvascular activation in the thyroid via the reactive oxygen species/nitric oxide-hypoxia-inducible factor-1α/vascular endothelial growth factor (VEGF) pathway. We hypothesized the additional involvement of mammalian target of rapamycin (mTOR) as a positive regulator o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1911
更新日期:2016-06-01 00:00:00
abstract::PTH increased PG synthase-2 transcription in osteoblastic MC3T3-E1 cells at 30 min, as assessed by nuclear run-on assays. To determine the signaling pathways used by PTH, the activity of a construct containing the PG synthase-2 gene between nucleotides -963 and +70 linked to a luciferase reporter was analyzed in stabl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5391
更新日期:1997-09-01 00:00:00
abstract::Gastrin is natriuretic, but its renal molecular targets and signal transduction pathways are not fully known. In this study, we confirmed the existence of CCKBR (a gastrin receptor) in male human renal proximal tubule cells and discovered that gastrin induced S6 phosphorylation, a downstream component of the phosphati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1813
更新日期:2013-02-01 00:00:00
abstract::To determine if the inhibitory effects of glucocorticoids on GnRH-stimulated secretion of LH observed in male rats in vivo are exerted directly on the pituitary, dispersed pituitary cells from adult male rats were treated with 60 or 600 ng/ml cortisol (F) or corticosterone (B) during one or two 48-h incubations. Contr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-855
更新日期:1985-09-01 00:00:00
abstract::Previously, we demonstrated that excision of the GnRH first intron (intron A) was largely attenuated in non-GnRH-producing tissues but accelerated in GnRH neurons. In the present study, we examined the splicing rate of GnRH pre-mRNA in developing normal mice and adult hypogonadal mice. The preoptic area and cerebral c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.10.8449
更新日期:2001-10-01 00:00:00
abstract::Mast cells are involved in atopic disorders, often exacerbated by stress, and are located perivascularly close to sympathetic and sensory nerve endings. Mast cells are activated by electrical nerve stimulation and millimolar concentrations of neuropeptides, such as substance P (SP). Moreover, acute psychological stres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5660
更新日期:1998-01-01 00:00:00
abstract::Glucocorticoids regulate hippocampal function in part by modulating gene expression through the glucocorticoid receptor (GR). GR binding is highly cell type specific, directed to accessible chromatin regions established during tissue differentiation. Distinct classes of GR binding sites are dependent on the activity o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1929
更新日期:2017-05-01 00:00:00
abstract::The quantities of estrone and estradiol were determined by radioimmunoassay in maternal and fetal plasma of the rhesus monkey from day 59 to 163 of gestation. A two way analysis of variance of data classified according to fetal sex and 3 pooled gestational ages for each hormone and for mother or fetus (4 analyses) rev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-425
更新日期:1975-08-01 00:00:00
abstract::The effects of diabetes on basal calcium metabolism and the response to endocrine stimulation were studied in hepatocytes from acute and long term diabetic rats. Hepatocyte calcium sequestration and turnover were increased in both acute and chronic diabetes. Cytosolic free calcium (Cai2+) was significantly increased i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2421
更新日期:1989-11-01 00:00:00
abstract::The Elovl3 gene belongs to the Elovl gene family, which encodes for enzymes involved in the elongation of very long chain fatty acids. The recognized role for the enzyme is to control the elongation of saturated and monounsaturated fatty acids up to 24 carbons in length. Elovl3 was originally identified as a highly ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1402
更新日期:2008-06-01 00:00:00
abstract::Testicular LH receptor occupancy and steroidogenic responses were measured in adult male rats after intracardiac injections of [125I]iodo-hCG (0.5--5 x 10(6) cpm) mixed with known amounts of nonradioactive hCG to yield doses ranging from 10 ng to 300 micrograms. Uptake of the hormone by the testis was measured in the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-3-982
更新日期:1982-09-01 00:00:00
abstract::We have investigated the effects of veratridine, a Na+ channel activator, and ouabain, an inhibitor of Na+-K+-ATPase, on short term (1-h) PRL release from primary cultures of rat anterior pituitary cells and from the rat anterior pituitary cell line GH4C1 in culture. Both compounds should increase intracellular Na+. V...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-4-1228
更新日期:1983-10-01 00:00:00
abstract::GH promotes the conversion of cultured preadipose 3T3 cells into adipose cells. The serum of most animals also promotes this differentiation. In order to determine the extent to which GH is responsible for the adipogenic activity of serum, we used a specific antiserum able to suppress completely the adipogenic activit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-527
更新日期:1984-02-01 00:00:00
abstract::The ability of ovarian steroids to influence the release of LH was examined after ovariectomy in regularly cycling adult female rats and two types of anovulatory persistent estrous rats: rats treated with 10 mug of testosterone propionate (TP) shortly after birth, and normal rats housed under constant illumination. Tw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-2-486
更新日期:1975-02-01 00:00:00
abstract::Estrogen formation is catalyzed by the aromatase cytochrome P450 (P450AROM) enzyme. Aromatase activity has been detected in several regions in the rat brain. In the present study, we used peptide-generated polyclonal antibodies raised against a 20-amino acid synthetic fragment of the rat P450AROM protein (as deduced f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-2834
更新日期:1991-12-01 00:00:00
abstract::Hyperactivation of the GHRH receptor or downstream signaling components is associated with hyperplasia of the pituitary somatotrope population, in which adenomas form relatively late in life, with less than 100% penetrance. Hyperplastic and adenomatous pituitaries of metallothionein promoter-human GHRH transgenic (Tg)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1482
更新日期:2009-07-01 00:00:00
abstract::The electrical responses of rat islet cells maintained in culture with varying levels of glucose were determined. After culture with 2.8 or 4.2 mM glucose, the beta-cells did not respond electrically to 27.8 mM glucose. However, after culture in 5.6 mM glucose, the cells responded to 27.8 mM glucose in short term expe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-5-1350
更新日期:1978-05-01 00:00:00