当前位置： SCI文献检索 > BRITISH JOURNAL OF CANCER期刊下所有文献 > The potential of carboxypeptidase G2-antibody conjugates as anti-tumour agents. I. Preparation of antihuman chorionic gonadotrophin-carboxypeptidase G2 and cytotoxicity of the conjugate against JAR choriocarcinoma cells in vitro.

The potential of carboxypeptidase G2-antibody conjugates as anti-tumour agents. I. Preparation of antihuman chorionic gonadotrophin-carboxypeptidase G2 and cytotoxicity of the conjugate against JAR choriocarcinoma cells in vitro.

Abstract：

:Carboxypeptidase G2, a zinc metalloenzyme isolated from Pseudomonas sp. strain RS-16, which catalyses the hydrolytic cleavage of reduced and non-reduced folates to pteroates and L-glutamate, has been linked to a monoclonal antibody (W14A) raised to human chorionic gonadotrophin. The coupling efficiency and retention of antibody and enzymatic activities are compared for three separate methods of preparing 1:1 conjugates. Preliminary in vitro studies on the cytotoxicity of the free enzyme and the conjugated enzyme towards JAR choriocarcinoma cells are reported. Despite the limitations of the in vitro model, it could be demonstrated that a significant proportion of 10(6) choriocarcinoma cells lost viability when exposed to either free or conjugated enzyme for 72 hours at concentrations of carboxypeptidase G2 of 1-3 units ml-1 of medium.

journal_name

Br J Cancer

journal_title

British journal of cancer

authors

Searle F,Bier C,Buckley RG,Newman S,Pedley RB,Bagshawe KD,Melton RG,Alwan SM,Sherwood RF

doi

10.1038/bjc.1986.62

subject

Has Abstract

pub_date

1986-03-01 00:00:00

pages

377-84

issue

3

eissn

0007-0920

issn

1532-1827

journal_volume

53

pub_type

杂志文章

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