Abstract:
:A mouse ear reaction for testing cell mediated immunity of human lymphocytes using the local graft-versus-host reaction assay is described. Five million human mononuclears were locally injected into the ears of immune suppressed NZW mice. The reaction mounted was quantitated by determining the 125I-Iodo-Deoxyuridine (125I-UdR) incorporation in both ears. The ratio of 125I-UdR incorporation, of the injected to that of the non-injected ear (GVHR index), 7 days after lymphocyte injection, served as an accurate measure for the extent of the reaction. Only normal human mononuclears and purified, separated normal human T lymphocytes mounted a local graft-versus-host reaction. Whereas normal human B lymphocytes, chronic lymphatic leukemia B lymphocytes, mononuclears from patients with transitional cell carcinoma of the bladder, irradiated normal human mononuclears, mouse syngeneic mononuclears, or human erythrocytes gave no positive reaction. These experiments demonstrate that this assay can be used to quantitate an in-vivo specific graft-versus-host reaction.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Mukamel E,Umiel T,Shohat B,Pecht M,Servadio C,Trainin Ndoi
10.1016/S0171-2985(85)80050-4subject
Has Abstractpub_date
1985-02-01 00:00:00pages
21-9issue
1eissn
0171-2985issn
1878-3279pii
S0171-2985(85)80050-4journal_volume
169pub_type
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