Cell-cycle-dependent expression of human melanoma membrane antigen analyzed by flow cytometry.

Abstract:

:Knowledge of tumor antigenic expression is crucial to the design of therapeutic strategy. A murine monoclonal antibody (BE4) against a human melanoma membrane antigen, was used to study the in vitro expression of this antigen. By membrane immunofluorescence, BE4 reacted against 5 of 8 melanoma lines as compared to zero of 13 other cell populations. Using flow cytometry, the antigenic M14 CEM melanoma cells consisted of 40% to 60% of the total cell population. Dual-parameter measurements of DNA content and membrane antigen demonstrated that the nonantigenic cells were predominantly in G0/G1 phase, whereas the antigenic cells were distributed throughout the cell cycle. Within one passage, the sorted and recultured nonantigenic population demonstrated a similar proportion of antigenic cells as the unsorted original population. It was concluded that the expression of human melanoma antigen was cell-cycle-dependent. Understanding factors that turn off the expression of antigen in G0/G1 phase may lead to better immunotherapeutic strategies.

journal_name

Cancer

journal_title

Cancer

authors

Leong SP,Bolen JL,Chee DO,Smith VR,Taylor JC,Benfield JR,Klevecz RR

doi

10.1002/1097-0142(19850315)55:6<1276::aid-cncr2820

subject

Has Abstract

pub_date

1985-03-15 00:00:00

pages

1276-83

issue

6

eissn

0008-543X

issn

1097-0142

journal_volume

55

pub_type

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