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Effects of exposure of DNA to methyl mercury on its activity as a template-primer for DNA polymerases.

Abstract：

:A previous publication [Frenkel, Cain, and Chao, Biochem. Biophys. Res. Commun. 127, 849-856 (1985)] described the observation that double-stranded DNA which was briefly exposed to methyl mercury (MeHg) and purified to remove free methyl mercury was transcribed at a higher rate by RNA polymerase II from wheat germ. The specificity of this phenomenon has now been investigated by examining the activity of this MeHg-exposed DNA as a template-primer for DNA polymerases. DNA synthesis by the bacteriophage T4-induced DNA polymerase was higher with the MeHg-exposed DNA as a template-primer than with control DNA. In contrast, the rate of DNA synthesis by E. coli DNA polymerase I was lower with the MeHg-exposed DNA as template-primer. With both enzymes (as well as with RNA polymerase II), after denaturation of the MeHg-exposed and control DNAs the differences in template activity were either eliminated or markedly reduced. The enzymes are thus able to detect a MeHg-induced alteration in DNA. In contrast, circular dichroism, a physical method that is sensitive to conformational changes in DNA, did not detect any difference between the MeHg-exposed and control DNAs.

journal_name

J Inorg Biochem

journal_title

Journal of inorganic biochemistry

authors

Frenkel GD,Wilson H,Ducote J

doi

10.1016/0162-0134(86)80012-5

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

113-21

issue

2

eissn

0162-0134

issn

1873-3344

pii

0162-0134(86)80012-5

journal_volume

27

pub_type

杂志文章

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