Abstract:
BACKGROUND:Genetic processes underlying fetal lung development and maturation are incompletely understood. Better knowledge of these processes would provide insights into the causes of lung malformations and prevention of respiratory distress syndrome and the potential adverse effects of glucocorticoids. Hox genes are involved in the lung branching morphogenesis and maturation of respiratory epithelium, but their expression pattern remains to be defined. OBJECTIVES:We hypothesized that genes involved in lung branching would be downregulated during early development, whereas those involved in maturation would be unchanged or upregulated. METHODS:TaqMan real-time primers and probes were designed for all 39 murine Hox genes, and the murine SP-B gene and transcription profiles of these genes were obtained from whole lungs isolated at e14.5, e16.5, e18.5, e19.5 and postnatal days 1 and 20. RESULTS:Hox genes in clusters A and B, specifically those between paralog groups 3 and 7, were the most represented, with Hoxa4 and Hoxa5 being the most highly transcribed. A wave of reduced transcription in 16 Hox genes, coincident with increased SP-B transcription, was observed with advancing gestation. Consistently high transcription of Hoxa5 from e14.5 to postnatal day 20 may indicate that sustained transcription is required for normal lung maturation. When e15.5 lungs were cultured with dexamethasone, Hoxb6, Hoxb7 and Hoxb8 levels were significantly upregulated, creating the potential for modulation of diverse downstream target genes. CONCLUSIONS:Improved understanding of the genetic processes underlying lung development afforded by our Q-PCR platform may allow development of more specific methods for inducing fetal lung maturation.
journal_name
Neonatologyjournal_title
Neonatologyauthors
Grier DG,Thompson A,Lappin TR,Halliday HLdoi
10.1159/000201739subject
Has Abstractpub_date
2009-01-01 00:00:00pages
50-60issue
1eissn
1661-7800issn
1661-7819pii
000201739journal_volume
96pub_type
杂志文章相关文献
Neonatology文献大全abstract::Noninvasive ventilation for preterm infants at birth has been recommended and universally adopted. The umbilical cord is often clamped immediately in order to provide the support the infant needs for stabilization. However, recent scientific data from experimental studies that involve animals in transition and human s...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000497421
更新日期:2019-01-01 00:00:00
abstract::Mechanical ventilation is a risk factor for cerebral inflammation and brain injury in preterm neonates. The risk increases proportionally with the intensity of treatment. Recent studies have shown that cerebral inflammation and injury can be initiated in the delivery room. At present, initiation of intermittent positi...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000444918
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Recurrent and prolonged seizures are harmful for the developing brain, emphasizing the importance of early seizure recognition and effective therapy. Amplitude-integrated electroencephalography (aEEG) has become a valuable tool to diagnose epileptic seizures, and, in parallel, genetic etiologies are increasi...
journal_title:Neonatology
pub_type: 杂志文章,多中心研究
doi:10.1159/000478651
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Serum ferritin measurements are used in clinical populations to estimate total body iron stores and the risk of subsequent iron deficiency or overload. The lack of normative newborn serum ferritin concentration data between 23 and 41 weeks has led to difficulty in establishing the incidence and degree of abn...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000100805
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Intermittent hypoxic episodes are common among preterm infants, although longer term consequences on growth pattern and cardiovascular regulation are unclear. Furthermore, the effects of intermittent hypoxia (IH) may depend on the pattern of hypoxia-reoxygenation. OBJECTIVES:We tested the hypothesis that a ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000338096
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:While hypoxic events with bradycardias are a frequent occurrence during the first weeks of life in preterm infants, the impact of preterm birth on maturation of the cardiovascular response to hypoxia in early postnatal life is unknown. OBJECTIVES:In the present study, we tested the hypothesis that preterm b...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000110868
更新日期:2008-01-01 00:00:00
abstract::We report the successful and safe use of levosimendan, a new calcium-sensitizing agent with positive inotropic and vasodilatory action, in 2 critically ill term newborns with acute heart failure and pulmonary hypertension in the absence of any underlying heart malformation and/or previous cardiosurgical procedures. Du...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000329848
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate whether a new MRI scoring system for preterm non-haemorrhagic white matter injury (WMI), derived from the analysis of the natural evolution of WMI throughout the neonatal period until term-equivalent age, can be used for outcome prediction. METHODS:Eighty-two infants <36...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000499346
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Heart rate (HR) detection in premature infants using electrocardiography (ECG) is challenging due to a low signal amplitude and the fragility of the premature skin. Recently, the dynamic light scattering (DLS) technique has been miniaturized, allowing noninvasive HR measurements with a single sensor. OBJECT...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000506771
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:High-dose recombinant erythropoietin (rEpo) is neuroprotective in neonatal animal models of brain injury, but the long-term consequences of neonatal exposure have not been studied. OBJECTIVES:We hypothesized that multiple injections of high-dose rEpo during the neonatal period would be safe, and would impro...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000096969
更新日期:2007-01-01 00:00:00
abstract::There is mounting evidence that early continuous positive airway pressure (CPAP) from birth is feasible and safe even in very preterm infants. However, many infants will develop respiratory distress syndrome (RDS) and require surfactant treatment. Combining a noninvasive ventilation approach with a strategy for surfac...
journal_title:Neonatology
pub_type: 杂志文章,评审
doi:10.1159/000381122
更新日期:2015-01-01 00:00:00
abstract:AIM:Melatonin is a potent free radical scavenger and an indirect antioxidant. Knowledge about the behavior of melatonin secretion in the early neonatal period, which may relate to its properties at a vital stage during very high antioxidant demands, is limited. PATIENTS AND METHODS:We studied 35 newborns admitted to t...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000102957
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Clinical illness caused by resistant bacteria usually represents a wider problem of asymptomatic colonization. Active surveillance with appropriate institution of isolation precautions represents a potential mechanism to control colonization and reduce infection. The neonatal intensive care unit (NICU) is an...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000343150
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Newborns are susceptible to inflammatory diseases due to defects in clearing activated immune cells from tissues. Therefore, mechanisms have likely evolved to protect neonates from leukocyte-mediated cytotoxicity. Bilirubin has antioxidant activity, and it is possible that it also exerts effects on cellular ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000343097
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Early host-microbe interaction provides important maturational stimuli for the developing immune system. The role of prenatal microbial contact remains elusive. OBJECTIVES:Our aim was to investigate whether microbes in placenta or amniotic fluid affect fetal innate immune gene expression during late pregnan...
journal_title:Neonatology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000339182
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Sustained lung inflation (SLI) applied at birth has been demonstrated to lead to clearance of lung fluid and achievement of a precocious functional residual capacity in animal studies. OBJECTIVES:To verify if the application of SLI in preterm infants at birth may reduce the need for mechanical ventilation a...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000298312
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Long-gap esophageal atresia (LGEA) may have clinical and syndromic presentations different from those of esophageal atresia (EA) that affects shorter segments of the esophagus (non-LGEA). This may suggest unique underlying developmental mechanisms. OBJECTIVES:We sought to characterize clinical differences b...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000449241
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:We hypothesized that color Doppler-measured cerebral blood flow velocity (CD-CBFV) as measured in the three feeding arteries of the brain can be used as an estimator of global cerebral blood flow. PATIENTS AND METHODS:CD-CBFV was determined as soon as possible after determination of phase-contrast magnetic ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000342336
更新日期:2013-01-01 00:00:00
abstract:AIM:To determine the prevalence of renal anomalies in patients with an isolated single umbilical artery (SUA). METHODS:We performed a retrospective study of all renal ultrasound examinations assessed at our centre between January 1998 and December 2008 in neonates with SUA with or without associated anomalies. RESULT...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000253151
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:To examine the impact of medical complexity among very preterm infants on health care resource use, family, and neurodevelopmental outcomes at 18 months' corrected age. METHODS:This observational cohort study of Canadian infants born < 29 weeks' gestational age in 2009-2011 compared infants with and those wi...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000496494
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Cardiac troponin T (cTnT) has been proposed as specific biochemical marker for myocardial infarction in adults. Cardiac function in neonates could be influenced by the severity of respiratory distress and its ventilatory management. OBJECTIVES:To establish a normal range of cTnT in healthy neonates, compare...
journal_title:Neonatology
pub_type: 临床试验,杂志文章
doi:10.1159/000100964
更新日期:2007-01-01 00:00:00
abstract::We describe three positive influenza AH1N1 cases in a neonatal unit during the influenza pandemic in Spain. One term baby presented with an upper respiratory tract infection, another preterm infant with an apnea episode following nosocomial infection, and thirdly, a term infant of a mother with influenza AH1N1 had sev...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000324232
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Apnea of prematurity (AOP) occurs frequently in preterm infants and a variable proportion of AOP can be induced by gastroesophageal reflux (GER). Conservative treatment, including dietary modifications, should be the first-line approach for both GER and GER-related apneas in this population. OBJECTIVES:To e...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000342703
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Inhaled glucocorticoids may increase the risk of pneumonia in adults. Thus, respiratory infections may be a potential explanation for the non-significantly increased mortality seen in the glucocorticoid group in the largest randomized trial on inhaled glucocorticoids for preventing bronchopulmonary dysplasia...
journal_title:Neonatology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000468507
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:International neonatal resuscitation guidelines recommend assessing chest excursion when the heart rate is not improving. However, the accuracy in assessing 'adequate' chest excursion lacks objectivity. AIM:It was the aim of this study to test the accuracy in the assessment of 'adequate' chest excursion by ...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000322009
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Transcutaneous bilirubin (TcB) devices are commonly used for screening of hyperbilirubinemia in term and near-term infants not exposed to phototherapy. However, the accuracy of TcB devices in infants exposed to phototherapy is unclear. OBJECTIVES:To conduct a systematic review of studies comparing TcB devic...
journal_title:Neonatology
pub_type: 杂志文章,meta分析,评审
doi:10.1159/000442195
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Periventricular white matter (PWM) is particularly vulnerable in very preterm infants. Non-cystic white matter injury, known as non-cystic periventricular leukomalacia (ncPVL), is the commonest 'lesion' affecting the preterm brain. There is no consensus about whether ncPVL can be reliably identified from cer...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000357937
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Carriers of Staphylococcus aureus strains can be the source of epidemic infection for patients. OBJECTIVES:A molecular epidemiological analysis of an impetigo bullosa outbreak in a neonatal ward was performed in order to determine a potential source of the infection and possible routes of subsequent spreadi...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000204961
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Phenobarbitone induces suppression of cerebral electrical activity on amplitude-integrated electroencephalography (aEEG) in neonates with hypoxic-ischemic encephalopathy (HIE); however, its effect during therapeutic hypothermia (TH) has not been well characterized. OBJECTIVE:To evaluate the effect of phenob...
journal_title:Neonatology
pub_type: 杂志文章
doi:10.1159/000511540
更新日期:2021-01-07 00:00:00
abstract::Despite recent advances in the perinatal management of neonatal respiratory distress syndrome (RDS), controversies still exist. We report the updated recommendations of a European panel of expert neonatologists who had developed consensus guidelines after critical examination of the most up-to-date evidence in 2007. T...
journal_title:Neonatology
pub_type: 杂志文章,实务指引
doi:10.1159/000297773
更新日期:2010-06-01 00:00:00