Expression of X-linked inhibitor of apoptosis protein in human colorectal cancer and its correlation with prognosis.

Abstract:

BACKGROUND:X-linked inhibitor of apoptosis protein (XIAP) is a member of the inhibitor of apoptosis family of proteins and deregulation of XIAP can result in tumorigenicity. The objective of this study was to evaluate the prognostic significance of XIAP expression in colorectal cancer (CRC). METHODS:RT-PCR was performed to detect the expression of XIAP mRNA in CRC cells and tissues. The expression of XIAP protein in tissues was measured by immunohistochemistry. The correlation of XIAP expression with clinicopathologic factors and prognosis of CRC patients was evaluated. RESULTS:CRC cells showed significantly higher levels of XIAP mRNA expression than normal human intestinal epithelial cell. The expression level of XIAP mRNA in CRC samples was significantly higher than that in corresponding non-tumor samples. XIAP staining was positive in the cytoplasm of CRC cells. Higher XIAP protein expression was significantly correlated with tumor differentiation (P = 0.016), venous invasion (P = 0.039), and Duke's staging (P = 0.002). Moreover, XIAP-high group showed lower disease-free (P = 0.0136) and overall survival (P = 0.0084) rates than XIAP-low group. Multivariate analysis indicated that the status of XIAP expression was an independent prognostic factor for CRC (P = 0.0206; HR: 2.730; 95% CI: 1.226-5.445). CONCLUSION:The status of XIAP expression might become an independent prognostic marker for CRC.

journal_name

J Surg Oncol

authors

Xiang G,Wen X,Wang H,Chen K,Liu H

doi

10.1002/jso.21408

subject

Has Abstract

pub_date

2009-12-15 00:00:00

pages

708-12

issue

8

eissn

0022-4790

issn

1096-9098

journal_volume

100

pub_type

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