Frequent K-ras mutations and absence of p53 mutations in mucin-producing tumors of the pancreas.

Abstract:

:Mucin-producing tumors of the pancreas (MPT) are characterized by the production of much mucin and a benign course after surgical treatment. We examined 16 cases of MPT and 20 cases of "common" pancreatic duct cell carcinomas (DCC) in regard to K-ras and p53 mutations. The mutations were detected by constant denaturant gel electrophoresis in combination with other techniques using PCR products amplified from the samples microdissected from the tissue sections. K-ras codon 12 mutations were identified in all MPT and in 95% of DCC. On the other hand, p53 mutations were found in four of 20 (20%) DCC, and p53 was immunocytochemically overexpressed in 3 of the 4 mutated cases. However, no p53 mutations and no p53 overexpression were identified in the 16 MPT. These results indicate that, although the K-ras codon 12 mutations may be almost essential for the development of both MPT and DCC, p53 mutations seemed to be involved mainly to the latters.

journal_name

J Surg Oncol

authors

Hoshi T,Imai M,Ogawa K

doi

10.1002/jso.2930550205

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

84-91

issue

2

eissn

0022-4790

issn

1096-9098

journal_volume

55

pub_type

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