Abstract:
INTRODUCTION:Excessive platelet activation fundamentally contributes to cardiovascular events and mortality in patients with diabetes mellitus. Functional resistance has been described for current antiplatelet therapies in broad populations that include patients with diabetes. We investigated acute and chronic effects of AZD6140, a reversible oral rapid-onset P2Y(12) antagonist, on platelet reactivity in diabetic rats. MATERIALS AND METHODS:Diabetes was induced by streptozotocin injection in male Wistar rats. After 4 weeks, AZD6140 was administered (5mg/kg by gavage) and achieved sufficient plasma levels within 30 minutes. Platelet reactivity was determined by ADP-induced P-selectin expression, aggregation and adhesion on fibrinogen coated membranes under arterial flow conditions. RESULTS:At 0.5 hour, AZD6140 strongly reduced ADP-induced P-selectin surface expression, inhibited ADP-induced platelet aggregation, and significantly reduced platelet adhesion to fibrinogen under arterial flow conditions. Chronic treatment with AZD6140 (10mg/kg bid for 2 weeks, based on data obtained in the acute study) starting at day 14 reduced P-selectin surface expression on circulating platelets, indicating lower in vivo platelet activation. Platelet reactivity was improved 12 hours after the last dose, while basal platelet activity remained reduced. AZD6140 was rapidly absorbed in diabetic rats and inhibited platelet reactivity. Chronic treatment lowered in vivo platelet activation of circulating platelets. CONCLUSION:AZD6140 inhibits platelet reactivity in diabetic rats rapidly and reversibly. Markers of tonic platelet activation, which were increased in diabetic rats, were lowered to levels comparable to non-diabetic rats following chronic treatment with AZD6140.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Flierl U,Schöpp C,Jaitner J,Bauersachs J,Schäfer Adoi
10.1016/j.thromres.2009.09.025subject
Has Abstractpub_date
2010-03-01 00:00:00pages
e93-9issue
3eissn
0049-3848issn
1879-2472pii
S0049-3848(09)00418-6journal_volume
125pub_type
杂志文章abstract::A direct assay for plasminogen activator (PA) was developed. It employed polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and beta-mercaptoethanol to monitor PA-mediated conversion of single chain, 125I-plasminogen to two chain plasmin. By incorporating Triton X-100, albumin and trasylol in...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90008-2
更新日期:1984-05-01 00:00:00
abstract::Pharmacovigilance is an essential element of any drug treatment and considering the history of adverse events due to products used to treat inherited bleeding disorders, it should be an integral component of modern haemophilia treatment. Because inherited bleeding disorders and adverse events are rare, a multicentre, ...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究
doi:10.1016/S0049-3848(10)70150-X
更新日期:2011-01-01 00:00:00
abstract:INTRODUCTION:Ultrasound can accelerate clot dissolution in vitro and in vivo. We used an ex vivo canine shunt to investigate low frequency ultrasound effects on platelet-rich stent thrombosis. METHODS AND RESULTS:Nitinol stents were expanded to 2 mm in diameter in two perfusion chambers in a parallel shunt and exposed...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2003.10.023
更新日期:2003-01-01 00:00:00
abstract::Analogs of the antithrombin peptide hirudin54-65 with C-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. The C-terminal residue, Gln65, could be replaced with L-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90090-x
更新日期:1989-05-15 00:00:00
abstract:INTRODUCTION:Bivalirudin is an alternative to heparin anticoagulation in infants and children in the setting of extracorporeal life support (ECLS). While activated partial thromboplastin time (aPTT) is widely accepted as the standard test to monitor bivalirudin therapy, the usefulness of thromboelastometry (ROTEM) to m...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2019.12.007
更新日期:2020-02-01 00:00:00
abstract:INTRODUCTION:The activation of coagulation is recognized as a universal event in severe sepsis. Both antithrombin and thrombomodulin play pivotal roles as suppressors of coagulation. Since the levels of both anticoagulants decrease significantly, we hypothesized that a combination therapy would be beneficial. METHODS:...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.04.015
更新日期:2014-07-01 00:00:00
abstract:INTRODUCTION:There is some controversy regarding the effect of CYP2C19 polymorphism on clinical outcome in patients with dual antiplatelet therapy. Chronic kidney disease (CKD) is associated with increased risk of cardiovascular event, but the association between the possession of CYP2C19 loss-of-function (LOF) alleles...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.07.039
更新日期:2014-11-01 00:00:00
abstract::Clinically unsuspected pulmonary embolism (UPE) is frequently diagnosed in cancer patients undergoing routine computed tomography scans for staging purposes or treatment response evaluation. The reported incidence of UPE ranges from 1% to 5% which probably represents an underestimation. A significant proportion of can...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(14)50028-X
更新日期:2014-05-01 00:00:00
abstract::Current therapies for treatment of hemophilia A involve infusion of factor VIII, but are ineffective for patients who develop inhibitory antibodies. We have previously proposed that bypassing the intrinsic pathway (VIIIa/IXa) with reversibly acylated factor Xa offers an improvement on existing therapies as it provides...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00267-3
更新日期:1997-11-15 00:00:00
abstract::Platelet prothrombin converting activity was measured in a system using washed human platelets and purified coagulation factors Xa, Va and prothrombin. Exposure of platelet prothrombin converting activity evoked by collagen or the combined action of collagen and thrombin was effectively inhibited when a collagen deriv...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90065-9
更新日期:1985-02-01 00:00:00
abstract::Lipopolysaccharide-containing outer membrane vesicles (OMV-LPS) which are spontaneously released from Neisseria meningitidis during logarithmic growth were studied for their ability to induce procoagulant (tissue factor), profibrinolytic (urokinase-type plasminogen activator) and antifibrinolytic (plasminogen activato...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(01)00256-0
更新日期:2001-05-15 00:00:00
abstract::Fibrinogen is an important risk factor for atherosclerosis, stroke and cardiovascular heart disease (CHD). This risk is increased when associated with a high serum cholesterol. Furthermore, it is also believed that not only fibrinogen concentration, but also the quality of fibrin networks may be an important risk fact...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0049-3848(97)00062-5
更新日期:1997-05-01 00:00:00
abstract::Heparin-induced thrombocytopenia (HIT) is a transient hypercoagulability state initiated, paradoxically, by the anticoagulant, heparin. It is characterized by antibody-induced activation of platelets, leading to thrombin generation. Many patients with HIT develop thrombosis; even when heparin is stopped because of "is...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(03)00336-0
更新日期:2003-05-01 00:00:00
abstract::Three commercial thrombin preparations used clinically to stop topical bleeding were studied. The specific activities of these preparations were 1,064 U/mg (human); 59 U/mg (bovine) and 147 U/mg (bovine). SDS-PAGE analysis of the human product produced one major band corresponding to a molecular weight of alpha-thromb...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90102-3
更新日期:1989-02-01 00:00:00
abstract::The role of protein kinase C (PKC) in platelet activation by thrombin was assessed using a PKC inhibitor Ro 31-7549/001 (R2) which, in vitro, shows more selectivity for PKC than other kinase inhibitors. During early (1.5 s) thrombin-induced platelet activation, when phosphorylation of 47 kDa protein (pleckstrin) and m...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90008-c
更新日期:1993-01-01 00:00:00
abstract:INTRODUCTION:Data regarding the clinical relevance of pulmonary infarction (PI) in patients with pulmonary embolism (PE) are lacking. The aim of this study was to investigate the clinical features of PE patients with PI and the prognostic role of PI for PE patients. MATERIALS AND METHODS:Based on computed tomography s...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.03.012
更新日期:2012-09-01 00:00:00
abstract::Diagnosis and risk stratification of patients with acute coronary syndromes remain extremely important in order to avoid unnecessary hospitalizations on the one hand, and to improve prognosis of these patients on the other. For diagnosis of acute coronary syndromes, an ECG should be obtained at rest, troponin T and I ...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(01)00298-5
更新日期:2001-09-30 00:00:00
abstract:BACKGROUND:Impaired endothelial-dependent flow-mediated dilatation (FMD) has been used to demonstrate endothelial dysfunction in a wide variety of cardiovascular disease, but previous studies have excluded patients with atrial fibrillation(AF). We therefore hypothesised that endothelial dysfunction exists in AF and tha...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.09.008
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVES:To evaluate the role of filter implantation in reducing the incidence of fatal pulmonary embolism during the endovascular treatment of thrombosis in the major tributary of the superior vena cava (SVC). METHODS:From October 2004 to October 2008, we conducted a cohort study of 40 patients with thrombosis of t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.04.009
更新日期:2014-07-01 00:00:00
abstract::The specific thromboxane A2/prostaglandin H2 (TXA2/PGH2) antagonist 13-azaprostanoic acid (13-APA) reverses platelet aggregation stimulated by TXA2/PGH2 and the prostaglandin endoperoxide analog U46619. The present report demonstrates that the deaggregatory properties of 13-APA are potentiated by prostacyclin (PGI2). ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(82)90097-4
更新日期:1982-12-15 00:00:00
abstract::One of the primary and most serious treatment-related complications in haemophilia A is the formation of anti-factor VIM (FVIII) antibodies, which significantly impacts patient care. For these patients, immunomodulatory therapy becomes important to induce immunological tolerance to FVIII. Immune tolerance induction (I...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(08)70003-3
更新日期:2008-01-01 00:00:00
abstract::The heparin-binding properties of human plasma apolipoproteins B-100 and E (apoB-100 and E) of low density lipoproteins (LDL), thrombin, and antithrombin-III (AT-III) were investigated. A highly reactive heparin (HRH) to apoB-100 was isolated by chromatography of crude heparin on a column of LDL immobilized to Affi-Ge...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90258-5
更新日期:1984-06-15 00:00:00
abstract::The effect of the major acute phase protein serum amyloid A (SAA) on collagen induced platelet aggregation was investigated. A truncated version of SAA, SAA25-76 was tested for inhibitory capacity of collagen induced platelet aggregation in platelet-rich plasma (PRP) and whole blood (WB). The tetrapeptide RGDV, a know...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90201-1
更新日期:1994-11-01 00:00:00
abstract::Both venous thromboembolism and intracranial metastases are common complications in the setting of primary brain tumors and metastatic malignancies. Anticoagulation is indicated in the presence of cancer-associated thrombosis in order to limit the risk of pulmonary embolism; however, there is reluctance to initiate an...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(16)30100-1
更新日期:2016-04-01 00:00:00
abstract::In adult patients with sickle cell disease two distinct subphenotypes have previously been defined: patients with the viscosity-vaso-occlusion subphenotype (VVO) suffer mainly from vaso-occlusive pain crises and have a relatively high hemoglobin concentration. Patients classified as the hemolysis-endothelial dysfuncti...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.10.006
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:It is well demonstrated that obesity is an independent risk factor for cardiovascular diseases. Recent studies have shown that obesity, insulin resistance and atherosclerosis are closely related phenomena in which low-grade inflammatory state and prothrombotic condition has pivotal roles. It has been shown t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.12.019
更新日期:2007-01-01 00:00:00
abstract::Fibrillar collagen endows the normal aortic wall with significant stiffness and strength and similarly plays important roles in many disease processes. For example, because of the marked loss of elastic fibers and functional smooth cells in aortic aneurysms, collagen plays a particularly important role in controlling ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.04.009
更新日期:2012-09-01 00:00:00
abstract::We evaluate the anti-platelet and anti-thrombotic effects of cilostazol using Multiplate® and PFA-100® in vitro and ex vivo with freshly isolated rat whole blood and in vivo venous and arterial thrombosis models in the same species, in an effort to assess the sensitivity of the whole blood aggregometer assays without ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.02.004
更新日期:2011-06-01 00:00:00
abstract::Soluble fibrin (SF) has attracted considerable interest as a marker for haemostatic activation. Two new enzyme immunoassays for SF, Enzymun-Test FM (Boehringer Mannheim, Germany) and Fibrinostika Soluble Fibrin (Organon Teknika, Belgium) have recently become commercially available. We measured plasma levels of SF in c...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00036-9
更新日期:1996-03-15 00:00:00
abstract::Studies with animal models implicate the plasma proteases factor XIIa (FXIIa) and α-kallikrein in arterial and venous thrombosis. As congenital deficiencies of factor XII (FXII) or prekallikrein (PK), the zymogens of FXIIa and α-kallikrein respectively, do not cause bleeding disorders, inhibition of these enzymes may ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2016.02.020
更新日期:2016-04-01 00:00:00