A non-viral vector for potential DMD gene therapy study by targeting a minidystrophin-GFP fusion gene into the hrDNA locus.

abstract：:The development and strategic application of effective anticancer therapies have turned out to be one of the most critical approaches of managing human cancers. Nevertheless, drug resistance is the major obstacle for clinical management of these diseases especially ovarian cancer. In the past years, substantial studie...

journal_title：Acta biochimica et biophysica Sinica

authors： Yung MM,Ngan HY,Chan DW

更新日期：2016-04-01 00:00:00

abstract：:Two full-length cDNAs, named CaM1a and CaM1b, encoding the highly conserved calmodulin1 (CaM1) proteins, were isolated from the cDNA library of amphioxus Branchiostoma belcheri tsingtauense. There are only two nucleotide differences between them, producing one amino acid difference between CaM1a and CaM1b. Comparison ...

journal_title：Acta biochimica et biophysica Sinica

authors： Luan J,Liu Z,Zhang S,Li H,Fan C,Li L

更新日期：2007-04-01 00:00:00

abstract：:The human immunodeficiency virus-1 (HIV-1) envelope glycoprotein 120 (gp120) binds to cell surface receptors and mediates HIV entry. Previous studies suggest the cell surface protein disulfide isomerase (PDI) might interact with disulfide bond(s) of gp120 and thus facilitate HIV-1 entry. In the present study, a kineti...

journal_title：Acta biochimica et biophysica Sinica

authors： Wang Z,Zhou Z,Guo ZY,Chi CW

更新日期：2010-05-15 00:00:00

abstract：:The recombinant peptide, DBAYL, a promising therapeutic peptide for type 2 diabetes, is a new, potent, and highly selective agonist for VPAC2 generated through site-directed mutagenesis based on sequence alignments of pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal peptide (VIP), and rela...

journal_title：Acta biochimica et biophysica Sinica

authors： Ma Y,Luo T,Xu W,Ye Z,Hong A

更新日期：2012-11-01 00:00:00

abstract：:The ability to self-renew is one of the most important properties of embryonic stem (ES) cells. Pluripotin (SC1), a small molecule with high activity and low toxicity, promotes self-renewal in mouse ES cells. SC1 can noticeably change the morphology of retinoic acid (RA)-induced F9 embryonic carcinoma cells (F9 cells)...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu Y,Ren X,Ke J,Zhang Y,Wei Q,Shi Z,Ai Z,Guo Z

更新日期：2018-08-01 00:00:00

abstract：:The critical molecular mechanism in the development of the pulmonary fibrosis remains unknown, leaving diagnosed patients with a poor prognosis. To isolate the genes specifically up-regulated in pulmonary fibrosis, we established a rat silicosis model 360 d after treatment with crystalline silica suspension. Radiograp...

journal_title：Acta biochimica et biophysica Sinica

authors： Jin Z,Liu B,Feng D,Chen C,Li X,Hu Y,Peng J,Liu Y,Du J,Fu C,Wen J

更新日期：2008-08-01 00:00:00

abstract：:Metastasis is the main reason for high recurrence and poor survival of hepatocellular carcinoma (HCC). The molecular mechanism underlying HCC metastasis remains unclear. In this study, we found that argininosuccinate synthase 1 (ASS1) expression was significantly decreased and down-regulation of ASS1 was closely corre...

journal_title：Acta biochimica et biophysica Sinica

authors： Tao X,Zuo Q,Ruan H,Wang H,Jin H,Cheng Z,Lv Y,Qin W,Wang C

更新日期：2019-03-01 00:00:00

abstract：:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) play a vital role in viral control and clearance. Recent studies have elucidated that Tapasin, an endoplasmic reticulum chaperone, is a well-known molecule that appears to be essential in peptide-loading process. The Janus kinase/signal transducers and ac...

journal_title：Acta biochimica et biophysica Sinica

authors： Wu S,Chen X,Tang Y,Zhang Y,Li D,Chen J,Wang J,Tang Z,Zang G,Yu Y

更新日期：2018-02-01 00:00:00

abstract：:In this study, a mistletoe lectin (ML) was purified from Chinese mistletoe and the effect of this 60 kDa Chinese ML on human gammadelta T cell cytotoxicity, apoptosis and modulation of the cytokine network was studied. The cytotoxic properties of delta T cells was evaluated by using a (51)Cr release test and employed ...

journal_title：Acta biochimica et biophysica Sinica

authors： Gong F,Ma Y,Ma A,Yu Q,Zhang J,Nie H,Chen X,Shen B,Li N,Zhang D

更新日期：2007-06-01 00:00:00

abstract：:Community-acquired pneumonia (CAP) is a major concern in hospitals and the bacterial community of which has not been systemically discussed yet. Sputum from patients in the acute stages is a kind of accessible sample reflecting its features. In our study, we analyzed 45 sputum samples from 45 patients with CAP. Eighte...

journal_title：Acta biochimica et biophysica Sinica

authors： Chen C,Shen T,Tian F,Lin P,Li Q,Cui Z,Zhang Y,Xue M,Ye J,Guo X,Zhou Y

更新日期：2013-12-01 00:00:00

abstract：:Dengue infection is a major cause of morbidity in tropical and subtropical regions, bringing nearly 40% of the world population at risk and causing more than 20,000 deaths per year. But there is neither a vaccine for dengue disease nor antiviral drugs to treat the infection. In recent years, dengue infection has been ...

journal_title：Acta biochimica et biophysica Sinica

authors： Qi RF,Zhang L,Chi CW

更新日期：2008-02-01 00:00:00

abstract：:Many harpins have been found in plant pathogen bacteria that can elicit disease and insect resistance in plants, and promote plant growth. In this work, we overexpressed and purified Xanthomonas oryzae pv. oryzae harpin, harpin(Xoo), in Escherichia coli BL21/pGEX-hpa1. Harpin(Xoo) was fused to the C-terminus of glutat...

journal_title：Acta biochimica et biophysica Sinica

authors： Li M,Shao M,Lu XZ,Wang JS

更新日期：2005-10-01 00:00:00

abstract：:Cotton fiber developmental transition from elongation to secondary cell wall biosynthesis is a critical growth shifting phase that affects fiber final length, strength, and other properties. Morphological dynamic analysis indicated an asynchronous fiber developmental pattern between two most important commercial cotto...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhou X,Hu W,Li B,Yang Y,Zhang Y,Thow K,Fan L,Qu Y

更新日期：2019-11-18 00:00:00

abstract：:Prion diseases are a group of transmissible fatal neurodegenerative disorders of humans and animals, including bovine spongiform encephalopathy, scrapie, and Creutzfeldt-Jakob disease. Microglia, the resident macrophages of the central nervous system, are exquisitely sensitive to pathological tissue alterations, alter...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhang S,Yang L,Kouadir M,Tan R,Lu Y,Chang J,Xu B,Yin X,Zhou X,Zhao D

更新日期：2013-09-01 00:00:00

abstract：:An L-amino acid oxidase (LAAO), NA-LAAO, was purified from the venom of Naja atra. Its N-terminal sequence shows great similarity with LAAOs from other snake venoms. NA-LAAO dose-dependently induced aggregation of washed human platelets. However, it had no activity on platelets in platelet-rich plasma. A low concentra...

journal_title：Acta biochimica et biophysica Sinica

authors： Li R,Zhu S,Wu J,Wang W,Lu Q,Clemetson KJ

更新日期：2008-01-01 00:00:00

abstract：:Metformin, a first line medication for type II diabetes, initially entered the spotlight as a promising anti-cancer agent due to epidemiologic reports that found reduced cancer risk and improved clinical outcomes in diabetic patients taking metformin. To uncover the anti-cancer mechanisms of metformin, preclinical stu...

journal_title：Acta biochimica et biophysica Sinica

authors： Saini N,Yang X

更新日期：2018-02-01 00:00:00

abstract：:Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hi...

journal_title：Acta biochimica et biophysica Sinica

authors： Jiang Y,Liu SZ,Zhang YL,Jiang MX,Sun QY,Chen DY

更新日期：2004-05-01 00:00:00

abstract：:Ubiquitin E3 ligase membrane-associated RING-CH-type finger 7 (MARCH7), also known as axotrophin, was originally identified in mouse embryonic stem cells. MARCH7 is involved in T-cell proliferation, neuronal development, and the immune system. However, its role in endometrial cancer (EC) remains unclear. This study ai...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu L,Hu J,Yu T,You S,Zhang Y,Hu L

更新日期：2019-05-23 00:00:00

abstract：:Transforming growth factor (TGF)-beta is a pleiotropic cytokine regulating a variety of cellular processes such as cell growth, differentiation, apoptosis, migration, cell adhesion, and immune response. In the well-understood classical TGF-beta signaling pathway, TGF-beta activates Smad signalling via its two cell sur...

journal_title：Acta biochimica et biophysica Sinica

authors： Yan X,Liu Z,Chen Y

更新日期：2009-04-01 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) is a Gram-positive pathogen which causes tuberculosis in both animals and humans. All tested rH-NS formulations induced a specific Th1 response, as indicated by increased production of interferon γ (IFN-γ) and interleukin 2 (IL-2) by lymphocytes in the spleen of mice which were immuniz...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu Y,Chen S,Pan B,Guan Z,Yang Z,Duan L,Cai H

更新日期：2016-10-01 00:00:00

abstract：:Castration-resistant prostate cancer (CRPC) is fatal and there is currently no effective clinical treatment. The antibiotic doxycycline has shown anti-cancer effect in several kinds of solid tumors including prostate cancer. In this study, a combination of doxycycline and doxorubicin was used to investigate the synerg...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhu C,Yan X,Yu A,Wang Y

更新日期：2017-11-01 00:00:00

abstract：:The plasminogen activator inhibitor type-2 (PAI-2) dependent apoptosis protection is due to the 33 amino acids fragment located between helix C and D of PAI-2, this fragment may interact with some unknown intracellular proteins. In this study we used the fragment between helix C and D of PAI-2 as a bait to perform a y...

journal_title：Acta biochimica et biophysica Sinica

authors： Fan J,Zhang YQ,Li P,Tong C,Tan L,Zhu YS

更新日期：2004-09-01 00:00:00

abstract：:Here we report that tgdA, a novel gene encoding a putative transglycosylase, affects both the morphological differentiation and the yield of blue-pigmented compound actinorhodin in Streptomyces coelicolor. The tgdA null mutant displays sparse aerial hyphae and irregular spore chains frequently lacking chromosomal DNA....

journal_title：Acta biochimica et biophysica Sinica

authors： Xie P,Zeng A,Lv X,Cheng Q,Qin Z

更新日期：2013-04-01 00:00:00

abstract：:Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. The progress of apoptosis is regulated in an orderly way by a series of signal cascades under certain circumstances. The caspase-cascade system plays vital roles in the induction...

journal_title：Acta biochimica et biophysica Sinica

authors： Fan TJ,Han LH,Cong RS,Liang J

更新日期：2005-11-01 00:00:00

abstract：:Although Ca(V)1.3 channels are known to be essential for neuronal excitation and signal transduction in the auditory system, their expression patterns in the cochlea are still not fully understood, particularly in the regions where non-sensory cells are located. We performed immunohistochemistry, western blotting and ...

journal_title：Acta biochimica et biophysica Sinica

authors： Chen J,Chu H,Xiong H,Chen Q,Zhou L,Bing D,Liu Y,Gao Y,Wang S,Huang X,Cui Y

更新日期：2012-06-01 00:00:00

abstract：:Acyl-coenzymeA:cholesterol acyltransferase 2 (ACAT2) is abundantly expressed in intestine and fetal liver of healthy human. Our previous studies have shown that in monocytic cells the low-level expression of human ACAT2 gene with specific CpG-hypomethylated promoter is regulated by the CCAAT/enhancer binding protein (...

journal_title：Acta biochimica et biophysica Sinica

authors： Guo D,Zhang X,Li Q,Qian L,Xu J,Lu M,Hu X,Zhu M,Chang CC,Song B,Chang T,Xiong Y,Li B

更新日期：2016-11-01 00:00:00

abstract：:The aim of this study is to construct a lentiviral expression vector containing a scavenger receptor (SR-PSOX) that binds with uniquely phosphatidylserine and oxidized lipoprotein with six histidine tags and to investigate the function of SR-PSOX in atherosclerosis. We utilize the ViraPower lentiviral expression syste...

journal_title：Acta biochimica et biophysica Sinica

authors： Quan Z,Yang H,Yang Y,Yan B,Cao R,Wen G,Liu C,Xu Y

更新日期：2007-03-01 00:00:00

abstract：:MicroRNAs have been reported to be closely related to the development of human lung cancers. However, the functions of microRNAs in non-small cell lung cancer (NSCLC) remain largely undefined. Here, we investigated the role of microRNA-193b (miR-193b) in NSCLC. Our data showed that miR-193b was markedly down-regulated...

journal_title：Acta biochimica et biophysica Sinica

authors： Hu H,Li S,Liu J,Ni B

更新日期：2012-05-01 00:00:00

abstract：:Peroxisome proliferator-activated receptor (PPAR) isoforms (alpha and gamma) are known to be expressed in pancreatic islets as well as in insulin-producing cell lines. Ligands of PPAR have been shown to enhance glucose-induced insulin secretion in rat pancreatic islets. However, their effect on insulin secretion is st...

journal_title：Acta biochimica et biophysica Sinica

authors： Xu ZK,Chen NG,Ma CY,Meng ZX,Sun YJ,Han X

更新日期：2006-01-01 00:00:00

abstract：:Recent studies have suggested that antibodies can catalyze the generation of unknown oxidants including hydrogen peroxide (H2O2) and ozone (O3) from singlet oxygen (1O2) and water. This study is aimed to detect the effect of antibody-catalyzed water oxidation on atherosclerosis. Our results showed that both H2O2 and O...

journal_title：Acta biochimica et biophysica Sinica

authors： Peng KJ,Huang YS,An LN,Han XQ,Zhang JG,Wang QL,Sun J,Wang SR

更新日期：2006-06-01 00:00:00