Abstract：
:One of the most well-characterised plant pathogenic interactions involves Arabidopsis thaliana and the bacteria Pseudomonas syringae pathovar tomato (Pst). The standard Pst inoculation procedure involves infiltration of large populations of bacteria into plant leaves which means that metabolite changes cannot be readily assigned to the host or pathogen. A plant cell-pathogen co-culture based approach has been developed where the plant and pathogen cells are separated after 12h of co-culture via differential filtering and centrifugation. Fourier transform infrared (FT-IR) spectroscopy was employed to assess the intracellular metabolomes (metabolic fingerprints) of both host and pathogen and their extruded (extracellular) metabolites (metabolic footprints) under conditions relevant to disease and resistance. We propose that this system will enable the metabolomic profiling of the separated host and pathogen (i.e. 'dual metabolomics') and will facilitate the modelling of reciprocal responses.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Allwood JW,Clarke A,Goodacre R,Mur LAdoi
10.1016/j.phytochem.2010.01.006subject
Has Abstractpub_date
2010-04-01 00:00:00pages
590-7issue
5-6eissn
0031-9422issn
1873-3700pii
S0031-9422(10)00013-0journal_volume
71pub_type
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