A comprehensive metabolite profiling of Isatis tinctoria leaf extracts.

Abstract:

:A broad-based characterisation of a pharmacologically active dichloromethane extract from Isatis tinctoria leaves was carried out. For a comprehensive picture we also included the polar constituents of I. tinctoria (MeOH extract) and for comparative purposes, the taxonomically closely related plant I. indigotica. Diode array detector, evaporative light scattering detector, atmospheric pressure chemical ionisation and electrospray ionisation mass spectrometry, and electrospray ionisation time-of-flight mass spectrometry detectors were used in parallel to ensure a wide coverage of secondary metabolites with highly diverging analytical properties. Off-line microprobe nuclear magnetic resonance spectroscopy after peak purification by semi-preparative high-pressure liquid chromatography served for structure elucidation of some minor constituents. More than 65 compounds belonging to various structural classes such as alkaloids, flavonoids, fatty acids, porphyrins, lignans, carotenoids, glucosinolates and cyclohexenones were unambiguously identified, and tentative structures were proposed for additional compounds. Numerous compounds were identified for the first time in the genus Isatis, and an indolic alkaloid was discovered.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Mohn T,Plitzko I,Hamburger M

doi

10.1016/j.phytochem.2009.04.019

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

924-34

issue

7

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(09)00172-1

journal_volume

70

pub_type

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