Abstract:
:The investigation of drug penetration into the stratum corneum (SC) by tape-stripping requires an accurate measure of the amount of SC on each tape-strip in order to determine the depth inside the SC. This study applies infrared densitometry (IR-D) to in vitro tape-stripping using the novel Squame Scan(R) 850A. The device had recently been shown to provide accurate measurements of the SC depth for tape-stripping in vivo. Furthermore, the suitability of IR-D for determining the endpoint of tape-stripping, i.e. complete SC removal, was tested. The SC depth was computed from the IR-D data of sequential tape-strips and compared to the results of a protein assay as gold standard. IR-D provided accurate depth results both for freshly excised skin and for skin stored frozen for up to 3 months. In addition, the lower limit of quantification of IR-D indicates the complete removal of the SC (less than 5% of the total SC remaining) and can be used for adjusting the number of tapes applied in situ. Therefore, IR-D is an accurate, fast and non-destructive method for SC depth determination.
journal_name
Skin Pharmacol Physioljournal_title
Skin pharmacology and physiologyauthors
Hahn T,Hansen S,Neumann D,Kostka KH,Lehr CM,Muys L,Schaefer UFdoi
10.1159/000288165subject
Has Abstractpub_date
2010-01-01 00:00:00pages
183-92issue
4eissn
1660-5527issn
1660-5535pii
000288165journal_volume
23pub_type
杂志文章abstract:PURPOSE OF THE STUDY:To investigate the role of paraoxonase 1 (PON1) and vitamin E in the pathogenesis of some autoimmune diseases, and to correlate their levels with the disease activity. PROCEDURES:This randomized case control study was performed on 60 subjects: 45 patients [suffering from psoriasis, vitiligo and al...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000342124
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Epicutaneous application of capsaicin causes a long-lasting analgesic effect by binding to the membrane transient receptor potential vanilloid 1 (TRPV1) on mechanoheat-sensitive C and Aδ fibres, changing axonal integrity and inhibiting neurogenic inflammatory processes. To date, no information is available re...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000362740
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The use of antioxidants has become a common practice in the development of antiaging cosmetics. OBJECTIVE:The aim of this study was to evaluate the clinical efficacy of cosmetic formulations containing lycopene and melatonin antioxidants. METHOD:Thirty-six healthy women from 32 to 65 years were enrolled in...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000508673
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Acrolein is a degradation product of lipid peroxide as well as a well-known environmental pollutant. As a hallmark of oxidatively damaged protein, protein carbonyl, including acrolein-protein adduct, has been observed in the skin. However, the influence of protein carbonylation on the stratum corneum (SC) ha...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000148042
更新日期:2008-01-01 00:00:00
abstract::Histone deacetylase inhibitors (HDACi), a relatively new group of epigenetic agents, are being investigated as powerful chemotherapeutics because of their antiproliferative and prodifferentiation effects both in vitro and in vivo, in various tumor cell lines. Only little is known with respect to their effects on norma...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,评审
doi:10.1159/000351363
更新日期:2014-01-01 00:00:00
abstract::Despite its remarkable barrier function, the skin remains an attractive site for systemic drug delivery given its easy accessibility, large surface area and the possibility to bypass the gastrointestinal tract and the liver and so modify drug absorption kinetics. The pioneering work of Scheuplein, Higuchi and others i...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,评审
doi:10.1159/000351815
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND/AIMS:Advanced glycation end products, which are linked to both aging and hyperglycemia, cause marked functional and structural alterations in human skin. Though it is well known that the metabolism of glucose is closely associated with that of fatty acid (FA), sharing the same energy-yielding reaction pathwa...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000448121
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIM:The skin, as the outermost organ, protects against exogenous hazards (outside-in barrier) and prevents the loss of essential parts of the body (inside-out barrier). The epidermal barrier exerts several functions with specific morphological elements. Regional differences in skin functions are well known. ...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000330721
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Dry skin induced by rheumatoid arthritis (RA) causes itching, which negatively influences a patient's quality of life. We previously reported that mast cells are related to dry skin in arthritic mice. However, the mechanism of mast cell activation is unclear. In this study, we examined the mechanism underlyi...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000488250
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The vitamin D endocrine system, besides multiple other functions, regulates aging in many tissues, including the skin. It protects the skin against the hazardous effects of many skin age-inducing agents, including ultraviolet radiation. Thus, in the present study we aimed to investigate the relationship betw...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000443839
更新日期:2016-01-01 00:00:00
abstract::Along with antibiotics, antihistamines are the most widely used systemic drugs in dermatology. This is attributable to the major role played by histamine in common diseases such as urticaria and atopic eczema. Of the currently recognised four subtypes of G protein-coupled histamine receptors, only the H1 and H2 subtyp...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,评审
doi:10.1159/000086667
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND/AIMS:Keloids result from aberrations in the normal wound healing cascade and can lead to pruritus, contractures and pain. The underlying mechanisms of excessive scarring are not yet understood, and most therapeutic strategies remain unsatisfactory. Psoriasin (S100A7) and koebnerisin (S100A15) are released by...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000363579
更新日期:2015-01-01 00:00:00
abstract:AIM:The purpose of this study was to characterise C₆₀-poly(vinylpyrrolidone) (PVP) dispersions, to analyse the cutaneous absorption of fullerenes as well as to evaluate whether UVA radiation (UVA-R) could modify its permeation profile. METHODS:Dispersions were characterised according to their pH, particle size, zeta p...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000362175
更新日期:2015-01-01 00:00:00
abstract::Biogenic amines are potential irritants e.g. in fish-, meat-, milk- and egg-processing professions like cooks, butchers and bakers. The aim of this study was to test the irritative and barrier-disrupting properties of the biogenic amines ammonium hydroxide (AM), dimethylamine (DMA) and trimethylamine (TMA). A repeated...
journal_title:Skin pharmacology and physiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000083709
更新日期:2005-03-01 00:00:00
abstract::Cutaneous irritation presents a major health problem with serious social and occupational impact. The interaction between an irritant and the human skin depends on multiple factors: the intrinsic properties and the nature of the irritant itself, and specific individual- and environment-related variables. The main path...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,评审
doi:10.1159/000131077
更新日期:2008-01-01 00:00:00
abstract::Neoplastic skin lesions are multifocal, diffuse skin infiltrations of particular relevance in the differential diagnosis of ulcerative, nodular, or crusting skin lesions. Nonmelanoma skin cancers (NMSCs), namely, basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and also actinic keratosis (AK), are the most c...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,评审
doi:10.1159/000479529
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND/AIMS:Extrinsic and intrinsic skin aging is subject to constant remodeling and degradation processes, primarily in components of the extracellular matrix. While collagen fibers thin out during the aging process, the amorphous elastin fibers accumulate. These are essential formative components of the dermis. S...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000477854
更新日期:2017-01-01 00:00:00
abstract::Brian Barry published over 300 research articles across topics ranging from colloid science, vasoconstriction and the importance of thermodynamics in dermal drug delivery to exploring the structure and organisation of the stratum corneum barrier lipids and numerous strategies for improving topical and transdermal drug...
journal_title:Skin pharmacology and physiology
pub_type: 传,历史文章,杂志文章
doi:10.1159/000351946
更新日期:2013-01-01 00:00:00
abstract::The skin pH is crucial for physiological skin functions. A decline in stratum corneum acidity, as observed in aged or diseased skin, may negatively affect physiological skin functions. Therefore, glycolic acid-containing water-in-oil (W/O) emulsions adjusted to pH 4 were investigated regarding their effect on normal o...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000439030
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:About 50% of the sun's radiation arriving on earth is visible light (VIS). For a long time, its effects on human health have not been fully investigated. But recently, the influence of VIS has gained more attention, especially regarding skin physiology. Studies showed various effects, many of them harmful, c...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000501131
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND AND AIM:Since the pharmacological effects of diclofenac (DF) are short-lived because of its short half-life, prolongation of the pharmacological effect in a topical formulation is needed for more appropriate clinical use. For the enhancement of dermal accumulation and prolongation of the pharmacological effe...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000501734
更新日期:2019-01-01 00:00:00
abstract::In a clinical study, the skin penetration properties of melatonin 0.01% in a cream and 0.01 and 0.03% in a solution were investigated by evaluation of the serum melatonin levels over a 24-hour time course in 15 healthy volunteers. Blood samples for melatonin measurements were taken at 9.00 a.m. before applying the tes...
journal_title:Skin pharmacology and physiology
pub_type: 临床试验,杂志文章
doi:10.1159/000078822
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Ultraviolet (UV) irradiation is well known to promote inflammation and pigmentation of skin. UVB mainly affects dermatitis and pigmentation. Coffee contains a number of polyphenols, such as caffeic acid (CA) and chlorogenic acid (CGA) but their in vivo bioactivity for photobiology remains unclear. METHODS:C...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000455237
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:Polymethylmethacrylate (PMMA) plates generally used for the in vitro testing of sunscreens have failed to yield a satisfactory correlation between the sun protection factor (SPF) in vitro and that in vivo. In the present study, various polymers were investigated as alternative substrates to PMMA plates. PROCED...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000464471
更新日期:2017-01-01 00:00:00
abstract::The human skin barrier is an important part of the skin's intactness and its functionality is a precondition for healthy skin. Ingredients in cosmetic formulations, especially penetration enhancers, can influence this barrier function as they transport active agents into deeper skin layers. In this study different cos...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000354919
更新日期:2014-01-01 00:00:00
abstract:AIM AND METHODS:Drug development in dermatotherapy and also development of transdermal therapeutic systems (TTS) demand high-predictive in vitro models to estimate drug levels in skin and systemic uptake. Here we compare three ready-to-use models, reconstructed human epidermis, split porcine skin and the perfused porci...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000081683
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Sesquiterpenes in Curcuma aeruginosa Roxb. inhibit steroid 5α-reductase and dihydrotestosterone production, and reverse androgenic alopecia. This study sought to show that a high sesquiterpene C. aeruginosa extract (CA-ext) retards axillary hair growth in women. METHODS:Thirty women (age 20-52 years) were r...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000486136
更新日期:2018-01-01 00:00:00
abstract::Squalene is a major sebum lipid which is easily peroxidized by ultraviolet A (UVA) irradiation, generating products that can stress keratinocytes. Prevention of squalene photooxidation with antioxidants could potentially help defend skin from environmental stress. Previously, we have systematically characterized butyl...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000501731
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:Retinoic acid (RA) and benzoyl peroxide (BP) were studied, comparing their keratolytic efficacy and water barrier disruption to that of salicylic acid (SA), a well-established keratolytic, under similar conditions. PATIENTS/METHODS:Six volunteers were included in this blinded study. Eleven randomized test s...
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000093984
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVES/AIMS:The influence of emulsion droplet size on the skin penetration of a model drug, tetracaine, was studied. For this purpose, in vitro dermal and transdermal delivery of tetracaine from 6 emulsions (3 macro-emulsions with droplet sizes >1 microm and 3 nano-emulsions with droplet sizes <100 nm) were tested....
journal_title:Skin pharmacology and physiology
pub_type: 杂志文章
doi:10.1159/000106076
更新日期:2007-01-01 00:00:00