Impact of Body Site, Age, and Gender on the Collagen/Elastin Index by Noninvasive in vivo Vertical Two-Photon Microscopy.

Abstract:

BACKGROUND/AIMS:Extrinsic and intrinsic skin aging is subject to constant remodeling and degradation processes, primarily in components of the extracellular matrix. While collagen fibers thin out during the aging process, the amorphous elastin fibers accumulate. These are essential formative components of the dermis. So far, these processes have been detected in vertical histological sections of invasive biopsies and recently in noninvasive horizontal scans. METHODS:In this pilot study, a modified noninvasive 2-photon microscope was applied to measure the collagen/elastin index of skin in vivo. The obtained images permit an immediate vertical survey and allow a conclusion on the dermal composition at once. The collagen/elastin index was quantified by the second harmonic to autofluorescence aging index of dermis (SAAID) depending on volunteers' age (18-66 years), gender, and body area. RESULTS:The highest SAAID was measured at the volar forearm as compared to the abdominal SAAID, which was significantly lower (p < 0.05). The gluteal region showed the significantly lowest SAAID (p < 0.05). The SAAID in female skin was higher compared to male skin and decreased with increasing age. CONCLUSION:These effects are to be considered in subsequent studies to be able to specifically detect and evaluate influences.

journal_name

Skin Pharmacol Physiol

authors

Czekalla C,Schönborn KH,Döge N,Jung S,Darvin ME,Lademann J,Meinke MC

doi

10.1159/000477854

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

260-267

issue

5

eissn

1660-5527

issn

1660-5535

pii

000477854

journal_volume

30

pub_type

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