Abstract:
:Mitochondria play a key role in cell physiology including cell differentiation and proliferation. We investigated the changes of mitochondrial biogenesis during Wnt-induced osteoblastic differentiation of murine mesenchymal C3H10T1/2 cells. Scanning electron microscopy demonstrated that activation of Wnt signaling by Wnt-3A conditioned medicum (CM) resulted in significant increase in the number of mitochondria in C3H10T1/2 cells. In addition, the induction of alkaline phosphatase (ALP) activities by Wnt-3A CM was accompanied by significant increase in mitochondrial mass (p<0.05), mitochondrial membrane potential (p<0.05), intracellular reactive oxygen species production (p<0.05), resting oxygen consumption rate (p<0.05), cellular ATP content (p< or =0.05) and mtDNA copy number (p<0.05) compared to the cells with control CM (L292-CM) treatment. Moreover, co-treatment with Dkk-1 or WIF-1, both of which are Wnt inhibitors, abrogated the Wnt-3A-induced ALP activities as well as mitochondrial biogenesis markers. Upregulation of mitochondrial biogenesis by overexpression of mitochondrial transcription factor A (Tfam) significantly enhanced Wnt-induced osteogenesis as measured by ALP activities. In contrast, inhibition of mitochondrial biogenesis by treatment with Zidovudine (AZT) resulted in significant inhibition of ALP activities. Finally, ALP activities in human osteosarcoma cell line devoid of mitochondrial DNA (rho(0) cells) was significantly suppressed both in basal and Wnt-3A stimulated state compared to those from mitochondria-intact cells (rho+ cells). As a mechanism for Wnt-mediated mitochondrial biogenesis, we found that Wnt increased the expression of PGC-1alpha, a critical molecules in mitochondrial biogenesis, through Erk and p38 MAPK pathway independent of beta-catenin signaling. We also found that increased mitochondrial biogenesis is in turn positively regulating TOPflash reporter activity as well as beta-catenin levels. To summarize, mitochodrial biogenesis is upregulated by Wnt signaling and this upregulation contributes to the osteoblastic differentiation of mouse mesenchymal C3H10T1/2 cells.
journal_name
Bonejournal_title
Boneauthors
An JH,Yang JY,Ahn BY,Cho SW,Jung JY,Cho HY,Cho YM,Kim SW,Park KS,Kim SY,Lee HK,Shin CSdoi
10.1016/j.bone.2010.04.593subject
Has Abstractpub_date
2010-07-01 00:00:00pages
140-50issue
1eissn
8756-3282issn
1873-2763pii
S8756-3282(10)01137-3journal_volume
47pub_type
杂志文章相关文献
BONE文献大全abstract::Insulin-dependent type 1 diabetes mellitus (IDDM) has been shown to alter the properties of bone and impair bone repair in both humans and animals. The objective of this study was the detailed histomorphometric evaluation of the influence of the diabetic metabolic state on bone formation and remodeling during bone def...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.03.011
更新日期:2004-07-01 00:00:00
abstract::Global gene expression profiling has been used to study the molecular mechanisms of increased bone remodeling caused by PHPT. This disease is a model for chronic over-stimulation of target organs by PTH due to an inappropriate overproduction of the hormone. Hyperactivity of osteoblasts and osteoclasts lead to increase...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.12.020
更新日期:2006-07-01 00:00:00
abstract:OBJECTIVE:To compare the effects of TNF-α and IL-17A on osteogenic differentiation of isolated fibroblast-like synoviocytes (FLS) from healthy donors, osteoarthritis (OA) and rheumatoid arthritis (RA) patients. METHODS:FLS were cultured in osteogenic medium, with and without TNF-α and/or IL-17A. Extracellular matrix m...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.04.008
更新日期:2016-06-01 00:00:00
abstract::Spinal cord injury (SCI) causes profound bone loss due to muscle paralysis resulting in the inability to walk. Sclerostin, a Wnt signaling pathway antagonist produced by osteocytes, is a potent inhibitor of bone formation. Short-term studies in rodent models have demonstrated increased sclerostin in response to mechan...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.04.019
更新日期:2012-09-01 00:00:00
abstract::Human vertebral cancellous bone from white males (N = 19), black males (N = 16), white females (N = 12), and black females (N = 17) was examined histologically for the presence, numerical density, and morphology of in vivo microscopic cracking (microdamage). Two patterns of microcracks, linear and cross-hatched, were ...
journal_title:Bone
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/8756-3282(96)88871-5
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:Preterm infants are at risk for impaired bone mineralization and growth in length later in life due to inadequate nutritional intake in the early postnatal period. OBJECTIVE:To investigate whether increased nutritional supplementation of calcium, phosphate and protein in Very Low Birth Weight (VLBW) infants...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.12.022
更新日期:2018-03-01 00:00:00
abstract::It was the aim of this retrospective analysis to examine the influence of low-dose monophasic oral contraceptives (OCs) on bone mineral density (BMD) of the femoral neck and of the spine in young female endurance athletes. Data on training intensity, dietary intake, menarche, menstrual cycle disorders, years of OC use...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.05.017
更新日期:2004-10-01 00:00:00
abstract::Recently the third generation aromatase inhibitors have proved their efficacy and tolerability compared with tamoxifen in the adjuvant treatment of women with hormone responsive early breast cancer. However, there is some concern about the possible negative impact of these drugs on bone. The aim of the study was to ev...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2006.06.027
更新日期:2007-01-01 00:00:00
abstract::The long prevailing view that obesity is generally associated with beneficial effects on the skeleton has recently been challenged. Apolipoprotein E (apoE) is known to influence both adipose tissue and bone. The goal of the current study was to examine the impact of apoE on the development of fat mass and bone mass in...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.07.002
更新日期:2010-10-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) were first described over 50 years ago as potent inducers of ectopic bone formation when administrated subcutaneously. Preclinical studies have extensively examined the osteoinductive properties of BMPs in vitro and new bone formation in vivo. BMPs (BMP-2, BMP-7) have been used in or...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115513
更新日期:2020-09-01 00:00:00
abstract::Leptin, a small polypeptide hormone secreted by the adipocytes, controls body weight and gonadal function by binding to a special receptor located in the hypothalamus. Observational studies have demonstrated a controversial association between leptin and bone mineral density (BMD), and functional studies of the relati...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.05.006
更新日期:2019-08-01 00:00:00
abstract::On the basis of previous investigations indicating that the distribution of osteonic and interstitial lamellae in a normal femur depends on its mechanical properties, a procedure has been devised to provide information on the distribution of lamellae in human femora diaphyses deformed by bending. To achieve this, exac...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(87)90009-3
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Bone scintigraphy used as a diagnostic technique frequently yields non-specific intense uptake by vertebrae. Bone biopsy may therefore be required to establish a diagnosis in cases of Paget's disease of bone. The aim of the study was to ascertain the sensitivity and specificity of two new images-heart sign a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2003.10.014
更新日期:2004-04-01 00:00:00
abstract::Osteocytes, the most abundant cells in the cortical bone matrix, are thought to have mechanosensory and chemosensory regulatory roles. Marotti theorized that osteocytes signal to osteoblasts to recruit them into the osteocyte lineage. Martin extended this theory, assuming that osteocytes display a general inhibitory e...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00245-x
更新日期:2003-11-01 00:00:00
abstract::Recent increasing evidence supports a role for neuronal type signaling in bone. Specifically glutamate receptors have been found in cells responsible for bone remodeling, namely the osteoblasts and the osteoclasts. While most studies have focused on ionotropic glutamate receptors, the relevance of the metabotropic glu...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.10.025
更新日期:2017-01-01 00:00:00
abstract:INTRODUCTION:Because osteoporotic patients are prone to fractures, it must be considered whether or not patients undergoing drug therapies should discontinue treatment after sustaining a non-vertebral fracture. This study has tested the effect of novel active vitamin D3 analog, 1alpha,25-dihydroxy-2beta(3-hydroxypropox...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.07.023
更新日期:2007-01-01 00:00:00
abstract::Subject motion during high-resolution peripheral quantitative computed tomography (HR-pQCT) causes image artifacts that affect morphological analysis of bone quality. The aim of our study was to determine effectiveness of techniques for quality control in the presence of motion in vivo including automated and manual a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.03.003
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:There is evidence that the extent of vertebral bone marrow adiposity increases caudally along the vertebral column in children and adolescents. However, no studies have examined the lipid composition of bone marrow along the vertebral column, which may uniquely influence bone acquisition and metabolism durin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115702
更新日期:2021-01-01 00:00:00
abstract:INTRODUCTION:The RANKL-OPG axis is a key regulator of osteoclastogenesis and bone turnover activity. Its contribution to bone resorption under altered mechanical states, however, has not been fully elucidated. Here we examined the role of OPG in regulating mechanically induced bone modeling in a rat model of orthodonti...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.04.194
更新日期:2007-09-01 00:00:00
abstract::Women with prevalent fractures have an increased risk of developing additional, incident fractures. This article examines the relation between the location of prevalent fractures within the spine and the risk of subsequent vertebral fractures. The subjects were 721 Japanese-American women of mean age 69.5 +/- 5.3 (SD)...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00176-8
更新日期:1999-03-01 00:00:00
abstract::Osteoclastic bone degradation depends on the activity of several proteolytic enzymes, in particular to those belonging to the classes of cysteine proteinases and matrix metalloproteinases (MMPs). Yet, several findings suggest that the two types of plasminogen activators (PA), the tissue- and urokinase-type PA (tPA and...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.07.004
更新日期:2008-11-01 00:00:00
abstract::High fat diets can have detrimental effects on the skeleton as well as cause intestinal dysbiosis. Exercise prevents high fat (HF) diet-induced obesity and also improves bone density and prevents the intestinal dysbiosis that promotes energy storage. Previous studies indicate a link between intestinal microbial balanc...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.03.024
更新日期:2019-01-01 00:00:00
abstract::The direct effects of physical activity on long bones are already recognized. However, little information is available regarding distant osseous sites, such as maxillary bone. We evaluated the influence of physical training on alveolar bone quality, with and without mechanically-induced load during orthodontic tooth m...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115496
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:Progression of an incomplete atypical femoral fracture (IF) to a complete fracture in patients on long term bisphosphonate (BP) therapy can be catastrophic. We aimed to determine the frequency and imaging features of IF in asymptomatic patients on long-term BP therapy and to identify distinguishing clinical ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.03.018
更新日期:2013-07-01 00:00:00
abstract::Bone morphogenetic protein (BMP) signaling in osteoblasts plays critical roles in skeletal development and bone homeostasis. Our previous studies showed loss of function of BMPR1A, one of the type 1 receptors for BMPs, in osteoblasts results in increased trabecular bone mass in long bones due to an imbalance between b...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115402
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Fibrodysplasia ossificans progressiva (FOP) is a catastrophic genetic disorder of progressive heterotopic ossification (HO). Assessment of functional mobility in FOP will be essential to support clinical trials of investigational agents. RESULTS:Of necessity, we developed a simple, rapidly-administered, cum...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.04.015
更新日期:2017-08-01 00:00:00
abstract::Bone sialoprotein (BSP) is a multifunctional extracellular matrix protein found in mineralized tissues, including bone, cartilage, tooth root cementum (both acellular and cellular types), and dentin. In order to define the role BSP plays in the process of biomineralization of these tissues, we analyzed cementogenesis,...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.05.007
更新日期:2015-09-01 00:00:00
abstract::The influence of porosity, osteon density, osteonal area, osteonal lamellar area, osteon size, and haversian canal size on the tension and shear fracture toughness, that is, the mode I and mode II strain energy release rate (GIc and GIIc), respectively, were investigated for the human femur and the tibia. The results ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00173-7
更新日期:1997-11-01 00:00:00
abstract::Mandibuloacral dysplasia (MAD) is a rare recessively inherited premature aging disease characterized by skeletal and metabolic anomalies. It is part of the spectrum of diseases called laminopathies and results from mutations in genes regulating the synthesis of the nuclear laminar protein, lamin A. Homozygous or compo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.06.004
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Chronic kidney disease (CKD) is commonly associated with mineral and bone metabolism disorders, but these are less frequently studied in non-dialysis CKD patients than in dialysis patients. We examined and described international variation in mineral and bone disease (MBD) markers and their tr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115058
更新日期:2019-12-01 00:00:00